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Licensed Unlicensed Requires Authentication Published by De Gruyter February 8, 2020

Senecio serratuloides extract prevents the development of hypertension, oxidative stress and dyslipidemia in nitric oxide-deficient rats

  • Charlotte Mungho Tata , Constance Rufaro Sewani-Rusike , Opeopluwa Oyehan Oyedeji , Fikile Mahlakata , Mathulo Shauli and Benedicta Ngwenchi Nkeh-Chungag EMAIL logo



Hypertension is a silent killer with no obvious signs and symptoms; thus, it is crucial to prevent its development. Oxidative stress and hyperlipidemia are associated risk factors for developing hypertension. This study aimed at investigating the role of a crude extract of Senecio serratuloides in preventing the development of hypertension, oxidative stress and hyperlipidemia in a rat model of nitric oxide deficiency.


Female Wistar rats were co-treated with Nω-Nitro L-arginine methyl ester (L-NAME) (40 mg/kg) and the hydroethanolic extract of S. Serratuloides (HESS150 or HESS300 mg/kg) for 4 weeks. Twenty-hour urine samples were collected weekly during the study. At the end of the study serum, heart and kidneys were harvested for biochemical and histopathological analysis.


The higher dose (300 mg/kg) of the extract was more effective in preventing increase in systolic (p<0.001) and diastolic (p<0.05) blood pressure. At the end of the treatment period HESS300 treated rats had significantly (p<0.01) higher concentration of creatinine (91.24 ± 6 mg/dL) in urine and significantly (6.36 ± 0.4 mg/24 h; 0.001) lower proteinuria compared to L-NAME control rats (55.75 ± 8 mg/dL and 18.92 ± 2 mg/24 h, respectively). Creatinine clearance and glomerular filtration rate were lower in the L-NAME control group compared to all treatment groups. HESS300 prevented L-NAME-induced decrease in serum angiotensin II concentration, significantly decreased malondialdehyde concentration in serum (p<0.05) and kidneys (p<0.001). It also significantly (p<0.001) decreased low-density lipoprotein concentration while increasing the concentration of high-density lipoprotein cholesterol. It showed cardio- and reno-protective effects and significantly (p<0.01) prevented collagen deposition in these target organs.


These findings demonstrate the potential of S. Serratuloides in protecting rats from developing hypertension, hyperlipidemia and oxidative stress.

  1. Research Funding: This work was supported by the South African National Research Foundation (Grant No: 97133) and the National Institute of Minority Health and Health Disparities/National Institutes of Health (Grant Number: 5T37MD001810).


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Supplementary Material

The online version of this article offers supplementary material (DOI:

Received: 2018-05-09
Accepted: 2019-06-20
Published Online: 2020-02-08

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