Abstract
Background
Belladonna and Pyrogenium are commonly used to treat fever in homeopathy. But in vivo antipyretic activity of these medicines is not reported yet. The study was conducted to evaluate the effectiveness of ultrahigh dilutions of Belladonna (Bell) and Pyrogenium (Pyro) in fever model of rabbits induced by Baker's yeast.
Methods
Healthy, local strain rabbits (♂ and ♀) were divided into seven groups (n=42): Normal control, negative control, standard control, pyro 1000c, pyro 200c, Bell 1000c and Bell 200c. Fever was induced by intra peritoneal injection of 135 mg/kg Baker’s yeast suspension. Rectal temperature was measured hourly. All the medicines were administered once a day. The results were expressed as mean ± SEM. ANOVA and least significant difference post hoc test were applied for checking the level of significance, p-value of ≤0.05 was considered significant statistically.
Results
Pyro in both potencies significantly reduced fever in rabbits compared to negative control group, while both potencies of Bell were ineffective. Paracetamol and Pyro 1000c reduced by 1.2 °C (39.7 ± 0.1 to 38.5 ± 0.1), while Pyro 200c reduced by 1 °C temperature (39.7 ± 0.5 to 38.7 ± 0.2).
Conclusions
Pyro possesses marked antipyretic activity in rabbit’s Baker's yeast fever model. It would embolden its clinical use in fever with more guarantee of its efficacy. However, caveat of small sample size necessitates replication of experiment in large sample size.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report or in the decision to submit the report for publication.
References
[1] Ahmad S, Rehman T, Abbasi WM. In vivo evaluation of antipyretic effects of some homeopathic ultra-high dilutions on Baker's yeast-induced fever on Similia principle. J-Aim. 2018;9:177–182.10.1016/j.jaim.2017.05.007Search in Google Scholar
[2] Ahmad S, Rehman T, Abbasi WM. Effect of homoeopathic ultrahigh dilutions of Aconitum napellus on Baker's yeast induced fever in rabbits. J Integr Med. 2017;15:209–13.10.1016/S2095-4964(17)60329-7Search in Google Scholar
[3] Passeti TA, Bissoli LR, Beltrame RL, Fonsceca F. Action of methicillin on the” in vitro” growth of bacteria Staphylococcus aureus methicillin-resistance previously treated with homeopathic dilutions. Int J High Dil Res. 2015;14:57–58.10.51910/ijhdr.v14i2.793Search in Google Scholar
[4] Bousta D, Soulimani R, Jarmouni I, Belon P, Falla J, Froment N, et al. Neurotropic, immunological and gastric effects of low doses of Atropa belladonna L., Gelsemium sempervirens L. and Poumon histamine in stressed mice. J Ethnopharmacol. 2001;74:205–15 .10.1016/S0378-8741(00)00346-9Search in Google Scholar
[5] Pedalino CMV, Perazzo FF, Carvalho JCT, Martinho KS, de O Massoco C, Bonamin LV. Effect of Atropa belladonna and Echinacea angustifolia in homeopathic dilution on experimental peritonitis. Homeopathy. 2004;93:193–98.10.1016/j.homp.2004.07.004Search in Google Scholar
[6] Sandri PF, Portocarrero AR, Ciupa L, Falkowski GFS, Benvenutti MJ, Rodrigues W, et al. Clinical and parasitological assessment in mice treated with highly diluted Atropa belladonna. Int J High Dil Res. 2014;13:122–24 .10.51910/ijhdr.v13i47.734Search in Google Scholar
[7] Frass M, Linkesch M, Banyai S, Resch G, Dielacher C, Löbl T, et al. Adjunctive homeopathic treatment in patients with severe sepsis: a randomized, double-blind, placebo-controlled trial in an intensive care unit. Homeopathy. 2005;94:75–80 .10.1016/j.homp.2005.01.002Search in Google Scholar
[8] Gibson D. Pyrogenium, a study. British Homeopathy Journal. 1968;57:192–94.10.1016/S0007-0785(68)80030-4Search in Google Scholar
[9] Burnett J. Fevers and blood-poisoning. and theirt treatment, with special reference to the use of Pyrogenium. Calcutta: Haren & brother, 1968.Search in Google Scholar
[10] Kauser M, Zarif N, Mirza M. Effect of Bacillinum and Pyrogenium on the bacterial flora of buccal cavity of Colisa fasciata. Proc Pak Congr Zool. 1992:417–422.Search in Google Scholar
[11] Rudolf E Pyrogenium und Lachesis in der Behandlung der Phlegmone bei Pferden. 1951.Search in Google Scholar
[12] Boukhobza F. Hepar sulfur, bistouri homéopathique et Pyrogenium au cabinet dentaire. La Revue d'Homéopathie. 2011;2:26–27.10.1016/S1878-9730(11)70059-7Search in Google Scholar
[13] Werthmann K. Biological therapy. J Nat Med. 1991;IX:97–102.Search in Google Scholar
[14] Shah P. In vitro evaluation of antimicrobial activity of Pyrogenium. Int J Life Sci Pharma Res. 2015;1:1–4.Search in Google Scholar
[15] Singh L, Gupta G. Antiviral efficacy of homoeopathic drugs against animal viruses. Br Homeopathy J. 1985;74:168–74.10.1016/S0007-0785(85)80063-6Search in Google Scholar
[16] Clausen J, van Wijk R, Albrecht H. Infection models in basic research on homeopathy. Homeopathy. 2010;99:263–70.10.1016/j.homp.2010.08.001Search in Google Scholar
[17] Ataŏglu H, Dŏgan MD, Mustafa F, Akarsu ES. Candida albicans and Saccharomyces cerevisiae cell wall mannans produce fever in rats: role of nitric oxide and cytokines. Life Scien. 2000;67:2247–56.10.1016/S0024-3205(00)00804-3Search in Google Scholar
[18] Ahmad S, Rehman T, Ababsi WM. In vivo evaluation of antipyretic effects of homoeopathic ultrahigh dilutions of Typhoidinum on baker's yeast-induced fever in comparison with Paracetamol. Indian J of Res in Homoeopathy. 2017;11:170.10.4103/ijrh.ijrh_81_16Search in Google Scholar
[19] Abbasi WM, Ahmad S, Perveen S, Rehman T. Preliminary phytochemical analysis and in vivo evaluation of antipyretic effects of hydromethanolic extract of Cleome scaposa leaves. eJTCM. 2018;8:147–49.Search in Google Scholar
[20] Morimoto A, Murakami N, Sakata Y, Watanabe T, Yamaguchi K. Functional and structural differences in febrile mechanism between rabbits and rats. J Physiol. 1990;427:227.10.1113/jphysiol.1990.sp018169Search in Google Scholar PubMed PubMed Central
[21] Toussaint K, Yang X, Zielinski M, Reigle K, Sacavage S, Nagar S, et al. What do we (not) know about how paracetamol (acetaminophen) works?. J Clin Pharm Ther. 2010;35:617–38 .10.1111/j.1365-2710.2009.01143.xSearch in Google Scholar PubMed
[22] Charan J, Kantharia N. How to calculate sample size in animal studies? J Pharmacol Pharmacother. 2013;4:303.10.4103/0976-500X.119726Search in Google Scholar PubMed PubMed Central
[23] Johnson-Delaney CA. Rabbits. Exotic companion medicine handbook for veterinarians. USA: Wingers Publishing Incorporated, 1996:96.Search in Google Scholar
[24] Netea MG, Kullberg BJ, Jw VDM. Circulating cytokines as mediators of fever. Clin Infect Dis. 2000;31:S178–S84.10.1086/317513Search in Google Scholar PubMed
[25] Bruguerolle B, Roucoules X. Time-dependent changes in body temperature rhythm induced in rats by brewer's yeast injection. Chronobiol Int. 1994;11:180–86.10.3109/07420529409057238Search in Google Scholar PubMed
[26] Enache-Angoulvant A, Hennequin C. Invasive Saccharomyces infection: a comprehensive review. Clinical Infectious Diseases. 2005;41:1559–68.10.1086/497832Search in Google Scholar PubMed
[27] Hahnemann S. Organon of medicine, 6th ed (Translated by William Boericke). New Delhi: B Jain Publishers, 1991:243–53.Search in Google Scholar
[28] Ahmad S, Hassan A, Abbasi WM, Rehman T. Phytochemistry and pharmacological potential of Cassia absus – a review. J Pharm Pharmacol. 2018;70:27–41.10.1111/jphp.12816Search in Google Scholar PubMed
[29] Conforti A, Bellavite P, Bertani S, Chiarotti F, Menniti-Ippolito F, Raschetti R. Rat models of acute inflammation: a randomized controlled study on the effects of homeopathic remedies. BMC Complement Altern Med. 2007;7:1.10.1186/1472-6882-7-1Search in Google Scholar PubMed PubMed Central
[30] Bellavite P. Complexity science and homeopathy: a synthetic overview. Homeopathy. 2003;92:203–12.10.1016/j.homp.2003.08.002Search in Google Scholar PubMed
[31] Del Giudice E, Preparata G, Vitiello G. Water as a free electric dipole laser. Phys Rev Lett. 1988;61:1085.10.1103/PhysRevLett.61.1085Search in Google Scholar PubMed
[32] Demangeat J-L. Gas nanobubbles and aqueous nanostructures: the crucial role of dynamization. Homeopathy. 2015;104:101–15.10.1016/j.homp.2015.02.001Search in Google Scholar PubMed
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