Skip to content
Accessible Unlicensed Requires Authentication Published by De Gruyter October 24, 2019

Evaluation of anxiolytic and hypoglycemic potential of Cissus adnata Roxb. in animal model

Mohammed Shamim Hasan, Md. Giash Uddin, Mohammed Shoibe, Abdullah Al Mahmud and Sujan Banik ORCID logo

Abstract

Background

This study was designed to evaluate the anxiolytic and hypoglycemic potential of methanolic extract of Cissus adnata Roxb. is a crucial medicinal plant used in many disorders belongs to Vitaceae family.

Methods

Elevated plus maze (EPM) test and hole board test was applied for the anxiolytic activity with the Swiss albino mice. The hypoglycemic activity was measured by the glucose tolerance test in mice model. The capacity to produce the desired effect of the plant extract (200 and 400 mg/kg) was compared with the anxiolytic drug of standard diazepam (1 mg/kg i.p.) and anti-diabetic drug glibenclamide (10 mg/kg i.p.), respectively.

Results

The phytochemical screening of Cissus adnata extract exposed the presence of carbohydrate, phenol, flavonoid, saponins, cardiac glycoside, tannin, and gum. The anxiolytic effect was detected in both experiments which significantly raised the number of head dips and the time spent in the open arm of the EPM (p<0.05) as the dose enlarged. Hypoglycemic study of the extracts shows better effect by reducing blood glucose level.

Conclusions

The better anxiolytic and hypoglycemic activities in the present study are due to the existence of various phytochemical constituents like saponins, flavonoids, terpenoids, phenols, and tannins in this methanolic extract.

Acknowledgments

We are grateful to Dr Shaikh Bokhtear Uddin, a proficient botanist of the University of Chittagong for the identification of plant material.

  1. Author contributions: Concept – S.B.; Design – S.B.; Supervision – S.B.; Resource – M.S.M., M.S.; Materials – A.L.M.; Data Collection and/or Processing – M.S.M, M.G.S.; Analysis and/or Interpretation – M.S.M., S.B.; Literature Search – M.S., M.G.S.; Writing –M.G.U.; Critical Reviews – M.S.M., S.B., M.G.U., M.S., A.L.M.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The authors are declared they have no completing of interest.

References

[1] Chen U, Hussain MS, Mazumder T, Naim Uddin SM, Banik S. Neuropharmacological evaluation of methanolic extract of Costus speciosus Linn. rhizome in Swiss albino mice. Asian Pac J Trop Biomed 2019;9:217–21.Search in Google Scholar

[2] Mishra SK, Sing PN, Dubey SD. Evaluation of CNS depressant activity of Capparis zeylenica Linn. root. Res J Med Plant 2011;5:738–46.Search in Google Scholar

[3] Netto SM, Warela RW, Fechine MF, Queiroga MN, Quintans-Júnior LJ. Anxiolytic-like effect of Rauvolfia ligustrina Willd. Ex Roem. & Schult., Apocynaceae, in the elevated plus-maze and hole-board tests. Braz J Pharm 2009;19:888–92.Search in Google Scholar

[4] O’Donovana A, Slavich GM, Epel ES, Neylan TC. Exaggerated neurobiological sensitivity to threat as a mechanism linking anxiety with increased risk for diseases of aging. Neurosci Biobehav Rev 2013;37:96–108.Search in Google Scholar

[5] Doukkali Z, Taghzouti K, Bouidida EH, Nadjmouddine M, Cherrah Y, Alaoui K. Evaluation of anxiolytic activity of methanolic extract of Urtica urens in a mice model. Behav Brain Funct 2015;11:19.Search in Google Scholar

[6] Islam A, Hussain MS, Sen N, Abedin F, Millat MS, Islam MS, et al. Investigation of in vitro thrombolytic and anti-helminthic activity and in vivo anxiolytic and antidepressant potentiality with phytochemical nature of methanolic extract of Leucas lavandulifolia. Sustainable Chem Pharm 2017;6:61–6.Search in Google Scholar

[7] Ghosh A, Banik S, Amin MN, Ahmed J. Evaluation of antinociceptive, antihyperglycemic and membrane stabilizing activities of Garcinia lancifolia Roxb. J Tradit Complement Med 2017;8:303–7.Search in Google Scholar

[8] Aba PE. Evaluation of hepatic glycogen content, some haematological and biochemical parameters of alloxan-induced diabetic rats treated with combinations of glibenclamide and G.latifolium extract. J Complement Integr Med 2017;14:20160078.Search in Google Scholar

[9] Shoibe M, Chy MN, Alam M, Adnan M, Islam MZ, Nihar SW, et al. In Vitro and In Vivo biological activities of Cissus adnata (Roxb.). Biomedicines 2017;5:63–80.Search in Google Scholar

[10] Rashid RB, Towsif FN, Bushra FA, Tahia F. Antioxidant, membrane stabilizing and cytotoxic activities of Cissus adnata (Roxb.). Dhaka Univ J Pharm Sci 2016;15:69–71.Search in Google Scholar

[11] Rahman MA, Bokhtear US, Wilcock CC. Medicinal plants used by Chakma tribe in hill tracts districts of Bangladesh. Int J Tradit Knowl 2007;6:508–17.Search in Google Scholar

[12] Gilani A, Rahman A. Trends in ethnopharmacology. J Ethnopharmacol 2005;100:43–9.Search in Google Scholar

[13] Ghosh A, Banik S, Islam MA. In vitro thrombolytic, anthelmintic, antioxidant and cytotoxic activity with phytochemical screening of methanolic extract of Xanthium indicum leaves. Bangladesh J Pharmacol 2015;10:854–9.Search in Google Scholar

[14] Sarker M, Banik S, Hussain S, Ghosh A, Hossain MS. In-vitro and in-vivo pharmacological activities with phytochemical evaluation of methanolic extract of Microcos paniculata stem barks. Curr Drug Ther 2016;11:142–9.Search in Google Scholar

[15] Adeyemi OO, Yemitan OK, Taiwo AE. Neurosedative and muscle-relaxant activities of ethyl acetate extract of Baphia nitida AFZEL. J Ethnopharmacol 2006;106:312–16.Search in Google Scholar

[16] Yemitan OK, Ajibade AM, Adeyemi OO. Anticonvulsant activity of Dalbergia saxatilis. Niger J Neurosci 2001;4:33–40.Search in Google Scholar

[17] Dhara AK, Pal S, Nag Chaudhuri AK. Psychopharmacological studies on Tragia involucrata root extract. Phytother Res 2002;16:326–30.Search in Google Scholar

[18] Pareek H, Sharma S, Khajja BS, Jain K, Jain GC. Evaluation of hypoglycemic and anti-hyperglycemic potential of Tridax procumbens (Linn.). BMC Complement Altern Med 2009;9:48.Search in Google Scholar

[19] Joy KL, Kuttan R. Anti-diabetic activity of Picrorrhiza kurroa extract. J Ethnopharmacol 1999;67:143–8.Search in Google Scholar

[20] Sermakkani M, Thangapandian V. Phytochemical screening for active compounds in Pedalium murex L. Recent Res Sci Technol 2010;2:110–14.Search in Google Scholar

[21] Mahmud AA, Banik S, Arefin S, Hasan MS, Singha RC. Evaluation of anxiolytic and hypoglycemic activity of methanolic extract of Ixora cuneifolia in an animal model. J Coast Life Med 2017;5:451–4.Search in Google Scholar

[22] Takeda H, Tsuji M, Matsumiya T. Changes in head-dipping behavior in the hole-board test reflect the anxiogenic and/or anxiolytic state in mice. Eur J Pharmacol 1998;350:21–9.Search in Google Scholar

[23] Martínez-Vázquez M, Estrada-Reyes R, Escalona AG, Velázquez IL, Martínez-Mota L. Antidepressant-like effects of an alkaloid extract of the aerial parts of Annona cherimolia in mice. J Ethnopharmacol 2012;139:164–70.Search in Google Scholar

[24] Li H, Zhou P, Yang Q, Shen Y, Deng J. Comparative studies on anxiolytic activities and flavonoid compositions of Passiflora edulis ‘edulis’ and Passiflora edulis ‘flavicarpa’. J Ethnopharmacol 2011;133:1085–90.Search in Google Scholar

[25] Rivera EM, Cid MP, Zunino P, Baiardi G, Salvatierra NA. Central α-and β-thujone: similar anxiogenic-like effects and differential modulation on GABAA receptors in neonatal chicks. Brain Res 2014;1555:28–35.Search in Google Scholar

[26] Bhattacharya SK, Satyan KS. Experimental methods for evaluation of psychotropic agents in rodents: I–Anti-anxiety agents. Indian J Exp Biol 1997;35:565–75.Search in Google Scholar

[27] Wagner H, Ott S, Jurcic K, Morton J, Neszmelyi A. Chemistry, 13C-NMR study and pharmacology of two saponins from Colubrina asiatica. Planta Med 1983;48:136–41.Search in Google Scholar

[28] Dubois MA, Ilyas M, Wagner H. Cussonosides A and B, two triterpene-saponins from Cussonia barteri. Planta Med 1986;52:80–3.Search in Google Scholar

[29] Kunyanga CN, Imungi JK, Okoth M, Momanyi C, Biesalski HK, Vadivel V. Antioxidant and antidiabetic properties of condensed tannins in acetonic extract of selected raw and processed indigenous food ingredients from Kenya. J Food Sci 2011;76:560–7.Search in Google Scholar

[30] Chakkravarthy BK, Gupta S, Gambir SS, Gode KD. Pancreatic beta cell regeneration. A novel mechanism of Marsupium Rxb. Indian J Pharmacol 1980;12:123–7.Search in Google Scholar

[31] Hamden K, Jaouadi B, Salami T, Carreau S, Bejar S, Elfeki A. Modulatory effect of fenugreek saponins on the activities of intestinal and hepatic disaccharidase and glycogen and liver function of diabetic rats. Biotechnol Bioprocess Eng 2010;15:745–53.Search in Google Scholar

[32] Chen ZH, Li J, Liu J, Zhao Y, Zhang P, Zhang MX, et al. Saponins isolated from the root of Panax notoginseng showed significant anti-diabetic effects in KK-Ay mice. Am J Chin Med 2008;36:939–51.Search in Google Scholar

[33] Goto T, Takahashi N, Hirai S, Kawada T. Various terpenoids derived from herbal and dietary plants function as PPAR modulators and regulate carbohydrate and lipid metabolism. PPAR Res 2010;2010:483958.Search in Google Scholar

Received: 2018-08-17
Accepted: 2019-09-06
Published Online: 2019-10-24

© 2019 Walter de Gruyter GmbH, Berlin/Boston