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Licensed Unlicensed Requires Authentication Published by De Gruyter October 24, 2019

Evaluation of anxiolytic and hypoglycemic potential of Cissus adnata Roxb. in animal model

  • Mohammed Shamim Hasan , Md. Giash Uddin , Mohammed Shoibe , Abdullah Al Mahmud and Sujan Banik ORCID logo EMAIL logo



This study was designed to evaluate the anxiolytic and hypoglycemic potential of methanolic extract of Cissus adnata Roxb. is a crucial medicinal plant used in many disorders belongs to Vitaceae family.


Elevated plus maze (EPM) test and hole board test was applied for the anxiolytic activity with the Swiss albino mice. The hypoglycemic activity was measured by the glucose tolerance test in mice model. The capacity to produce the desired effect of the plant extract (200 and 400 mg/kg) was compared with the anxiolytic drug of standard diazepam (1 mg/kg i.p.) and anti-diabetic drug glibenclamide (10 mg/kg i.p.), respectively.


The phytochemical screening of Cissus adnata extract exposed the presence of carbohydrate, phenol, flavonoid, saponins, cardiac glycoside, tannin, and gum. The anxiolytic effect was detected in both experiments which significantly raised the number of head dips and the time spent in the open arm of the EPM (p<0.05) as the dose enlarged. Hypoglycemic study of the extracts shows better effect by reducing blood glucose level.


The better anxiolytic and hypoglycemic activities in the present study are due to the existence of various phytochemical constituents like saponins, flavonoids, terpenoids, phenols, and tannins in this methanolic extract.

Correction note

Correction added after online publication October 24, 2019: Mistakenly this article was already published ahead of print under with following affiliations: “1 Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh, E-mail:,,, 2 Department of Pharmacy, International Islamic University Chittagong Permanent Campus, Chittagong 4203, Bangladesh, E-mail:”


We are grateful to Dr Shaikh Bokhtear Uddin, a proficient botanist of the University of Chittagong for the identification of plant material.

  1. Author contributions: Concept – S.B.; Design – S.B.; Supervision – S.B.; Resource – M.S.M., M.S.; Materials – A.L.M.; Data Collection and/or Processing – M.S.M, M.G.S.; Analysis and/or Interpretation – M.S.M., S.B.; Literature Search – M.S., M.G.S.; Writing –M.G.U.; Critical Reviews – M.S.M., S.B., M.G.U., M.S., A.L.M.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The authors are declared they have no completing of interest.


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Received: 2018-08-17
Accepted: 2019-09-06
Published Online: 2019-10-24

© 2019 Walter de Gruyter GmbH, Berlin/Boston

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