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Licensed Unlicensed Requires Authentication Published by De Gruyter January 23, 2020

In vitro and in vivo anti-diabetic activity of Citrullus colocynthis pulpy flesh with seeds hydro-ethanolic extract

  • Aymen Owais Ghauri EMAIL logo , Saeed Ahmad and Tayyeba Rehman



Diabetes is the one of the leading cause of morbidity and mortality. Traditionally phytotherapy is widely being used for diabetes treatment and highly valued. Citrus colocynthis has known anti-diabetic potential. However, anti-diabetic potential of hydro-ethanolic extract of C. colocynthis pulpy flesh with seeds is not reported yet.


The extract of C. colocynthis pulpy flesh with seeds was done by maceration method using 70% ethanol. To evaluate anti-diabetic and antioxidant potential of the seeded fruit in vitro, α-glucosidase and DPPH inhibition assays was done, respectively. In vivo study used streptozotocin (STZ) induced diabetes model of rats. Rats were randomized in five groups i. e. normal control, negative control, standard control, C. colocynthis 150 and 300 mg/kg. STZ was administered to all groups except normal control. After wards, plant extract and glibenclamide is continued for 14 days. Blood samples were collected from rat tail vein daily and from Cardiac puncture at the end of study. The blood glucose levels were monitored daily by using one-touch blood glucose monitoring system. The blood glucose level was monitored on 0, 1st, 5th, 8th, 11th, and 14th day of induction.


Hydro-ethanolic extract of C. colocynthis pulpy flesh with seeds was able to decolorize DPPH and therefore possess antioxidant potential, continuous administration for 14 days showed a marked decrease in serum glucose levels (p 0.01) it is found to be somewhat less effective as glibenclamide (standard control) (p 0.001). A time-dependent decrease in blood glucose levels was observed (351.3 ± 4 to 258 m/kg).


Hydro-ethanolic extract of C. colocynthis pulpy flesh with seeds lowered the serum triglyceride and cholesterol levels in diabetic rats significantly as compared to negative control. The hypoglycemic effect of hydro-ethanolic extract of C. colocynthis pulpy flesh with seeds is may be due to α-glucosidase inhibition potential.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


[1] Shi C, Karim S, Wang C, Zhao M, Murtaza G. A review on antidiabetic activity of Citrullus colocynthis Schrad. Acta Pol Pharm 2014;71:363.Search in Google Scholar

[2] Patel D, Prasad S, Kumar R, Hemalatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed 2012;2:320–30.10.1016/S2221-1691(12)60032-XSearch in Google Scholar

[3] Nishikawa T, Edelstein D, Du XL, Yamagishi S-I. Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature 2000;404:787.10.1038/35008121Search in Google Scholar

[4] Mohan V, Venkatraman JV, Pradeepa R. Epidemiology of cardiovascular disease in type 2 diabetes: The Indian scenario. J Diabetes Sci Technol 2010;4:158–70.10.1177/193229681000400121Search in Google Scholar

[5] Kumar S, Kumar D, Jusha M, Saroha K, Singh N, Vashishta B. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract. Acta Pharm 2008;58:215–20.10.2478/v10007-008-0008-1Search in Google Scholar

[6] Heydari I, Radi V, Razmjou S, Amiri A. Chronic complications of diabetes mellitus in newly diagnosed patients. Int J Diabetes Mellit 2010;2:61–3.10.1016/j.ijdm.2009.08.001Search in Google Scholar

[7] Barnes PM, Bloom B, Nahin RL. Statistics NCfH. Complementary and alternative medicine use among adults and children: United States, 2007. MD: US Department of Health and Human Services, Centers for Disease Control and Prevention, National Center for Health Statistics Hyattsville, 2008.Search in Google Scholar

[8] Rivera J, Loya A, Ceballos R. Use of herbal medicines and implications for conventional drug therapy medical sciences. Altern Integr Med 2013;2013:1–6.Search in Google Scholar

[9] Rahimi R, Amin G, Ardekani MR. A review on Citrullus colocynthis Schrad.: From traditional Iranian medicine to modern phytotherapy. J Altern Complementary Med 2012;18:551–4.10.1089/acm.2011.0297Search in Google Scholar

[10] Shahabi S, Hassan ZM, Mahdavi M, Dezfouli M, Rahvar MT, Naseri M, et al. Hot and Cold natures and some parameters of neuroendocrine and immune systems in traditional Iranian medicine: A preliminary study. J Altern Complementary Med 2008;14:147–56.10.1089/acm.2007.0693Search in Google Scholar

[11] Ardekani MR, Rahimi R, Javadi B, Abdi L, Khanavi M. Relationship between temperaments of medicinal plants and their major chemical compounds. J Traditional Chin Med 2011;31:27–31.10.1016/S0254-6272(11)60006-XSearch in Google Scholar

[12] Jayaraman R, Shivakumar A, Anitha T, Joshi V, Palei N. Antidiabetic effect of petroleum ether extract of Citrullus colocynthis fruits against streptozotocin-induced hyperglycemic rats. Rom J Biol Plant Biol 2009;4:127–34.Search in Google Scholar

[13] Salami M, Aqanouri Z, Karimian M, Enshai M. The hypoglycemic effect of Citrullus colocynth in normal rats. Iran J Pharm Res 2010;3:50.Search in Google Scholar

[14] Dallak M, Bin-Jaliah I. Antioxidant activity of Citrullus colocynthis pulp extract in the RBC’s of alloxan-induced diabetic rats. Pak J Physiol 2010;6:1–5.Search in Google Scholar

[15] Mohammad D, Al-Khateeb M, Riyadh E, Al-Hashem F, Nabil B, Mohammad K. In vivo, acute, normo-hypoglycemic, antihyperglycemic, insulinotropic actions of orally administered ethanol extract of Citrullus colocynthis (L.) Schrab pulp. Am J Biochem Biotechnol 2009;5:119–26.10.3844/ajbbsp.2009.119.126Search in Google Scholar

[16] Khalil M, Mohamed G, Dallak M, Al-Hashem F, Sakr H, Eid RA, et al. The effect of Citrullus colocynthis pulp extract on the liver of diabetic rats a light and scanning electron microscopic study. Am J Biochem Biotechnol 2010;6:155–63.10.3844/ajbbsp.2010.155.163Search in Google Scholar

[17] Babiker HA, Eldin IM, Abd Elwahab HM. The effects of ethanolic extract of handal (Citrullus colocynthis L.) Fruit pulp on blood glucose level in normal fasting rats. Gezira J Health Sci 2012;8:1–7.Search in Google Scholar

[18] Abbasi WM, Ahmad S, Perveen S, Rehman T. Preliminary phytochemical analysis and in vivo evaluation of antipyretic effects of hydromethanolic extract of Cleome scaposa leaves. eJTCM 2018;8:147–9.Search in Google Scholar

[19] McDonald S, Prenzler PD, Antolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem 2001;73:73–84.10.1016/S0308-8146(00)00288-0Search in Google Scholar

[20] Ahmad S, Rehman T, Abbasi WM, Zaman MM. Analysis of antioxidant activity and total phenolic content of some homeopathic mother tinctures. Indian J Res Homeopathy 2017;11:21–5.10.4103/0974-7168.200843Search in Google Scholar

[21] Oueslati A, Rigane G, Ghazghazi H, Salem RB, Hamdi S, Hannachi M, et al. Phenolic content, antioxidant and antimicrobial activities of Trachyspermum ammi aerial parts growing wild in the north of Tunisia. J N Sci 2016;25:1151–60.Search in Google Scholar

[22] Meléndez NP, Nevárez-Moorillón V, Rodríguez-Herrera R, Espinoza JC, Aguilar C, Obal N. A microassay for quantification of 2, 2-diphenyl-1-picrylhydracyl (DPPH) free radical scavenging. Afr J Biochem 2014;8:14–18.10.5897/AJBR2013.0669Search in Google Scholar

[23] Moradi-Afrapoli F, Asghari B, Saeidnia S, Ajani Y, Mirjani M, Malmir M, et al. In vitro α-glucosidase inhibitory activity of phenolic constituents from aerial parts of Polygonum hyrcanicum. Daru 2012;20:1–6.10.1186/2008-2231-20-37Search in Google Scholar PubMed PubMed Central

[24] Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. The ARRIVE guidelines animal research: Reporting in vivo experiments. PLoS Biol 2010;8:e1000412.10.1111/j.1476-5381.2010.00872.xSearch in Google Scholar PubMed PubMed Central

[25] Charan J, Kantharia N. How to calculate sample size in animal studies? J Pharmacol Pharmacother 2013;4:303.10.4103/0976-500X.119726Search in Google Scholar PubMed PubMed Central

[26] Zhang M, Lv X-Y, Li J, Xu Z-G, Chen L. The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Exp Diabetes Res 2009;2008:1–9.10.1155/2008/704045Search in Google Scholar PubMed PubMed Central

[27] Pandikumar P, Babu NP, Ignacimuthu S. Hypoglycemic and antihyperglycemic effect of Begonia malabarica Lam. in normal and streptozotocin induced diabetic rats. J Ethnopharmacol 2009;124:111–15.10.1016/j.jep.2009.04.001Search in Google Scholar PubMed

[28] Shafaei H, Esmaeili A, Rad JS, Delazar A, Behjati M. Citrullus colocynthis as a medicinal or poisonous plant: A revised fact. J Med Plants Res 2012;6:4922–7.10.5897/JMPR11.264Search in Google Scholar

[29] Ojiako OA, Chikezie PC, Ogbuji AC. Radical scavenging potentials of single and combinatorial herbal formulations in vitro. J Tradit Complement Med 2016;6:153–9.10.1016/j.jtcme.2014.11.037Search in Google Scholar PubMed PubMed Central

[30] Luo Q, Cai Y, Yan J, Sun M, Corke H. Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum. Life Sci 2004;76:137–49.10.1016/j.lfs.2004.04.056Search in Google Scholar

[31] Gurudeeban S, Satyavani K, Ramanathan T. Alpha glucosidase inhibitory effect and enzyme kinetics of coastal medicinal plants. Bangladesh J Pharmacol 2012;7:186–91.10.3329/bjp.v7i3.11499Search in Google Scholar

[32] Kazeem MI, Ashafa AO. In-vitro antioxidant and antidiabetic potentials of Dianthus basuticus Burtt Davy whole plant extracts. J Herb Med 2015;5:158–64.10.1016/j.hermed.2015.06.003Search in Google Scholar

[33] Grover J, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol 2002;81:81–100.10.1016/S0378-8741(02)00059-4Search in Google Scholar

[34] Nikkilä EA, Kekki M. Plasma triglyceride transport kinetics in diabetes mellitus. Metabolism 1973;22:1–22.10.1016/0026-0495(73)90024-3Search in Google Scholar

[35] Dewanjee S, Das AK, Sahu R, Gangopadhyay M. Antidiabetic activity of Diospyros peregrina fruit: Effect on hyperglycemia, hyperlipidemia and augmented oxidative stress in experimental type 2 diabetes. Food Chem Toxicol 2009;47:2679–85.10.1016/j.fct.2009.07.038Search in Google Scholar PubMed

[36] Arulmozhi S, Mazumder PM, Lohidasan S, Thakurdesai P. Antidiabetic and antihyperlipidemic activity of leaves of Alstonia scholaris Linn. R. Br. Eur J Integr Med 2010;2:23–32.10.1016/j.eujim.2009.12.001Search in Google Scholar

[37] Iso H, Naito Y, Sato S, Kitamura A, Okamura T, Sankai T, et al. Serum triglycerides and risk of coronary heart disease among Japanese men and women. Am J Epidemiol 2001;153:490–9.10.1093/aje/153.5.490Search in Google Scholar PubMed

Received: 2018-12-04
Accepted: 2019-04-04
Published Online: 2020-01-23

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