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Licensed Unlicensed Requires Authentication Published by De Gruyter September 23, 2020

Exploring the phytochemical and nutraceutical potentials of dasapatrachurnam

  • Rasajna Nadella ORCID logo EMAIL logo , Daniel Hernandez-Baltazar ORCID logo , John Sushma Nannepaga ORCID logo , Balamani Venkata Annapurna Gorthi ORCID logo and Daniel Martinez-Fong ORCID logo



Dasapatrachurnam (DPC), a multicurative powder prepared from the leaves of 10 green leafy vegetables, was developed recently with known ethnobotanical and ethnopharmacological significance. However, its functional role in curing a disease is not yet scientifically proven. The present study aims at performing the phytochemical screening of DPC and exploring its possible activity as bacteriostatic, antineoplastic and anti-inflammatory.


We performed qualitative and Fourier transform infrared spectroscopy (FTIR) to find out the presence of active compounds and tested the bacteriostatic activity in four bacterial strains namely Bacillus subtilis, Escherichia coli, Streptococcus pyogenes and Staphylococcus aureus by agar well diffusion method. We further explored the antineoplastic activity in vitro in C6 and HEK293 cell lines by cell viability assay and the anti-inflammatory activity in the ovalbumin-induced inflammation in male Wistar rats.


DPC showed 60% solubility in PBS and showed the presence of flavonoids and glycosides. FTIR results indicated the presence of alkyl, ketone and aldehyde groups. The bacteriostatic activity of DPC was higher (60%) in E.coli and lower (8%) in S.aureus, when compared to streptomycin. The anti-cancerous activity of DPC in C6 and HEK293 cancer cells was similar to their respective positive controls, curcumin and camptothecin. The anti-inflammatory activity of DPC was more evident with local administration in all the parameters studied in brain hippocampus, kidney, liver and spleen in ovalbumin-induced rats.


Our results, for the first time, suggest the potentiality of the DPC in treating bacterial diseases, cancer and also inflammation. Our results also suggest the possible therapeutic role of DPC in treating chronic kidney disease.



Bowman’s capsule


Vascular congestion




Central vein


Destruction of Bowman’s capsule


Degenerative changes


Dimethyl sulfoxide




Enzyme linked immunosorbent assay


Fetal bovine serum


Fourier transform infrared spectroscopy


Germinal centers


Granular layer


Human embryonic kidney


Mild degenerative changes


Molecular layer


Marginal sinus


3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide


Marginal zone


Necrotic cells


Phosphate buffered saline


Proximal tubule


Regenerative changes


Renal tubule


Sinus region


Sinusoid space




World Health Organization


The authors would like to thank DST-CURIE, Sri Padmavathi Mahila Visvavidyalayam (SPMVV) for providing the microscope facility. We are grateful to Dr. Ramakrishna, Director of Sri Satyadeva Nursery, Kadiyam, East Godavari District, Andhra Pradesh, India for supplying us seedlings, soil and pots. However, neither DST-CURIE nor this nursery had a role in study design, data collection and analysis, decision to publish or preparation of the manuscript.

  1. Author contributions: RN funded, from personal earnings, and contributed in experimental design, conducted all the experimental work and drafting the manuscript. DHB prepared Figure 4, conducted statistical analysis and wrote statistical data in the manuscript. JSN helped us in providing the FTIR and microscopy along with rat facilities and analyzed FTIR data. BVAG formulated DPC and has done the phytochemical study. DMF helped us with MTT cell cytotoxicity assay. All the figures, images and work are original. All the authors commented, read and approved the final manuscript.

  2. Research funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The authors have had no financial, personal or other relationships with other people or organiations within 5 years of the beginning of the submitted work that could inappropriately influence, or be perceived to influence, their work. All the authors declared that no competing interests exist.


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Supplementary Material

The online version of this article offers supplementary material (DOI:

Received: 2018-12-14
Accepted: 2019-09-10
Published Online: 2020-09-23

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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