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Licensed Unlicensed Requires Authentication Published by De Gruyter July 26, 2019

Warionia saharae induces antihypertensive and vasorelaxant activities through nitric oxide and KATP channels pathways in rats

  • Fadwa EL-Ouady and Mohamed Eddouks EMAIL logo



Warionia saharae Bentham & Cosson. is used in traditional medicine to treat many diseases including hypertension.


The present study was designed to investigate the antihypertensive activity of aqueous extract of Warionia saharae (WSLAE) in anesthetized L-NAME (Nω-L-arginine methyl ester)-induced rats. The acute and sub-chronic effects of WSLAE on systolic, diastolic, mean arterial blood pressure and heart rate (HR) were investigated after oral administration of the extract (100 mg/kg body weight of WSLAE) during 6 h for the acute experiment and during 7 days for the sub-chronic test. In addition the effect of WSLAE on the contractile response of isolated rat aortic rings was tested and the role of the vascular receptors was evaluated using several drugs.


The results showed that WSLAE induced a significant decrease in the systolic, diastolic, mean arterial blood pressure and HR in both normotensive and hypertensive rats. Furthermore, this extract induced relaxation in rings pre-contracted with Epinephrine (10 µM) or with KCl (100 mM). This vasorelaxant capacity seems to be mediated through inhibition of KATP channels and nitric oxide (NO) synthase pathways.


This study supports the use of Warionia saharae in the management of hypertension in Moroccan traditional medicine.



diastolic blood pressure


heart rate


Nω-L-arginine methyl ester


mean blood pressure




systolic blood pressure


aqueous extract of Warionia saharae

W. saharae

Warionia sahare

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The research has been funded by the Centre National pour la Recherche Scientifique et Technique (CNRST) under grant N° PPR/2015/35.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

  6. Ethical approval: All applicable guidelines for the care and use of animals were followed (FSTE/2015).


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Received: 2019-01-23
Accepted: 2019-04-24
Published Online: 2019-07-26

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