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Licensed Unlicensed Requires Authentication Published by De Gruyter September 6, 2019

Oral supplementation with geranium oil or anise oil ameliorates depressed rat-related symptoms through oils antioxidant effects

  • Karima A. El-Shamy , Khaled M. M. Koriem EMAIL logo , Nevein N. Fadl , Marwa H. A. El-Azma , Mahmoud S. S. Arbid , Fatma A. Morsy , Salwa R. El-Zayat , Eman N. Hosny and Eman R. Youness



Depression is a psychiatric disease condition and the chronic mild stress (CMS) model is a well-known and valuable animal model of depression. Geranium oil and anise oil were chosen for such a study. The aim of this research was to establish the geranium oil and anise oil effect to ameliorate CMS-related symptoms.


This research included 80 male albino rats each group of 10 rats and the animals were divided into two major groups: normal and CMS. The normal group was subdivided into four (control, geranium oil, anise oil and venlafaxine drug) subgroups treated orally with saline, geranium oil, anise oil and venlafaxine drug, respectively, for 4 weeks. The CMS group was subdivided into four (CMS without any treatment, CMS + geranium oil, CMS + anise oil and CMS + venlafaxine drug) subgroups treated orally with geranium oil, anise oil and venlafaxine drug, respectively, for 4 weeks.


The sucrose consumption in sucrose preference test, the distance traveled test and center square entries test were decreased, while center square duration test, immobility time in tail suspension test and floating time in forced swimming test were increased in CMS. The superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase and catalase levels decreased but malondialdehyde and nitric oxide levels increased in brain cerebral cortex and hippocampus areas in CMS. The oral intake of geranium oil and anise oil pushes all these parameters to approach the control levels. These results were supported by histopathological investigations of both brain cerebral cortex and hippocampus tissues.


Geranium oil and anise oil ameliorate CMS-related symptoms and this effect were related to the antioxidant effects of oils.


The authors appreciate and thank the National Research Centre, Cairo, Egypt for the grant provided to start and complete this research.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The research was funded by the National Research Centre in Cairo, Egypt under number 11040102.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


[1] Serretti A, Mandelli L, Lattuada E, Smeraldi E. Depressive syndrome in major psychoses: a study on 1351 subjects. Psychiatry Res 2004;127:85–99.10.1016/j.psychres.2003.12.025Search in Google Scholar

[2] Tan YM, Cheung G. Self-harm in adults: a comparison between the middle-aged and the elderly. N Z Med J 2019;132:15–29.Search in Google Scholar

[3] Peet M, Murphy B, Shay J, Horrobin D. Depletion of omega-3 fatty acid levels in red blood cell membranes of depressive patients. Biol Psychiatry 1998;43:315–19.10.1016/S0006-3223(97)00206-0Search in Google Scholar

[4] Willner P. Reliability and utility of the chronic mild stress model of depression: a 10-year review and evaluation. Psychopharmacol (Berl.) 1997;134:319–29.10.1007/s002130050456Search in Google Scholar

[5] Hill MN, Hellemans KG, Verma P, Gorzalka BB, Weinberg J. Neurobiology of chronic mild stress: parallels to major depression. Neurosci Biobehav Rev 2012;36:2085–117.10.1016/j.neubiorev.2012.07.001Search in Google Scholar

[6] Zhang ZJ. Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sci 2004;75:1659–99.10.1016/j.lfs.2004.04.014Search in Google Scholar

[7] Papp M, Willner P, Muscat R. An animal model of anhedonia: attenuation of sucrose consumption and place preference conditioning by chronic unpredictable mild stress. Psychopharmacology (Berl.) 1991;104:255–9.10.1007/BF02244188Search in Google Scholar

[8] Lin T, Meng Y, Ji Z, Jiang H, Shao W, Gao R, et al. Extent of depression in juvenile and adolescent idiopathic scoliosis patients during treatment with braces. World Neurosurg 2019;126:e27–e32. DOI: 10.1016/j.wneu.2019.01.095.Search in Google Scholar

[9] Shellie RA, Marriott PJ. Comprehensive two-dimensional gas chromatography-mass spectrometry analysis of Pelargonium graveolens essential oil using rapid scanning quadrupole mass spectrometry. Analyst 2003;128:879–83.10.1039/b304371aSearch in Google Scholar

[10] Jain N, Aggarwal KK, Syamasundar KV, Srivastava SK, Kumar S. Essential oil composition of geranium (Pelargonium sp.) from the plains of Northern India. Flavour Fragrance J 2001;16:44–6.10.1002/1099-1026(200101/02)16:1<44::AID-FFJ943>3.0.CO;2-XSearch in Google Scholar

[11] Gupta R, Mallavarapu GR, Banerjee S, Kumar S. Characteristics of an isomenthone-rich somaclonal mutant isolated in a geraniol-rich rose-scented geranium accession of Pelargonium graveolens. Flavour Fragrance J 2001;16:319–24.10.1002/ffj.1002Search in Google Scholar

[12] Krzyśko-Łupicka T, Walkowiak W, Białoń M. Comparison of the Fungistatic activity of selected essential oils relative to Fusarium graminearum isolates. Molecules 2019;24:311.10.3390/molecules24020311Search in Google Scholar PubMed PubMed Central

[13] Cathcart-Rake EJ, Smith D, Zahrieh D, Loprinzi CL. Rose geranium in sesame oil nasal spray: a treatment for nasal vestibulitis? BMJ Support Palliat Care 2018. DOI: 10.1136/bmjspcare-2018-001569. [Epub ahead of print].Search in Google Scholar PubMed

[14] Abouhosseini Tabari M, Hajizadeh Moghaddam A, Maggi F, Benelli G. Anxiolytic and antidepressant activities of Pelargonium roseum essential oil on Swiss albino mice: possible involvement of serotonergic transmission. Phytother Res 2018;32:1014–22.10.1002/ptr.6038Search in Google Scholar PubMed

[15] Egyptian pharmacopoeia. General organization for government printing, 3rd ed. Cairo, Egypt: Cairo University, 1972:123–7.Search in Google Scholar

[16] Technical Centre for Agricultural and Rural Cooperation. African pharmacopoeia, Vol. 1. Lagos, Nigeria: Organization of African Unity, Scientific, Technical and Research Commission, 1986:58–61. ISBN: 978-2453-02-01.Search in Google Scholar

[17] De Guzman CC, Siemonsma JS. editors. Plant resources of South-East Asia, spices, vol. 13. Bogor, PROSEA, Leiden, Netherlands: Backhuis Publishers, 1999:81–6.Search in Google Scholar

[18] Youngken HW. Textbook of pharmacognosy, 6th ed. Philadelphia, PA: Blakiston, 1950:116–20. Search in Google Scholar

[19] British herbal pharmacopoeia. Exeter, British Herbal Medicine Association, 1996:240–6.Search in Google Scholar

[20] Blumenthal M. The complete German Commission monographs, 3rd ed. Austin, TX: American Botanical Council, 1998:76–82.Search in Google Scholar

[21] Da Rocha Neto AC, Beaudry R, Maraschin M, Di Piero RM, Almenar E. Ouble-bottom antimicrobial packaging for apple shelf-life extension. Food Chem 2019;279:379–88.10.1016/j.foodchem.2018.12.021Search in Google Scholar PubMed

[22] Tan H, Wooh S, Butt HJ, Zhang X, Lohse D. Porous supraparticle assembly through self-lubricating evaporating colloidal ouzo drops. Nat Commun 2019;10:478.10.1038/s41467-019-08385-wSearch in Google Scholar PubMed PubMed Central

[23] Aydin E, Hritcu L, Dogan G, Hayta S, Bagci E. The effects of inhaled Pimpinella peregrina essential oil on scopolamine-induced memory impairment, anxiety, and depression in laboratory rats. Mol Neurobiol 2016;53:6557–67.10.1007/s12035-016-9693-9Search in Google Scholar PubMed

[24] Mosaffa-Jahromi M, Tamaddon AM, Afsharypuor S, Salehi A, Seradj SH, Pasalar M. et al. Effectiveness of anise oil for treatment of mild to moderate depression in patients with irritable bowel syndrome: a randomized active and placebo-controlled clinical trial. J Evid Based Complementary Altern Med 2017;22:41–6.10.1177/2156587216628374Search in Google Scholar PubMed PubMed Central

[25] Guide for the Care and Use of Laboratory Animals. Environment, Housing, and Management. National Research Council (US) Committee for the update of the guide for the care and use of laboratory animals, 8th ed. Washington (DC): National Academies Press (US), 2011:62–147.Search in Google Scholar

[26] Boukhris M, Bouaziz M, Feki I, Jemai H, El Feki A, Sayadi S. Hypoglycemic and antioxidant effects of leaf essential oil of Pelargonium graveolens L’Hér. in alloxan induced diabetic rats. Lipids Health Dis 2012;11:1–10.10.1186/1476-511X-11-81Search in Google Scholar PubMed PubMed Central

[27] Koriem KM, Arbid MS, El-Gendy NF. The protective role of anise oil in oxidative stress and genotoxicity produced in favism. J Diet Suppl 2016;13:505–21.10.3109/19390211.2015.1119775Search in Google Scholar PubMed

[28] Suttle NF. Copper deficiency in ruminants; recent developments. Vet Rec 1986;119:519–22.10.1136/vr.119.21.519Search in Google Scholar PubMed

[29] Pagalia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70:158–69.Search in Google Scholar

[30] Goldberg DM, Spooner RJ. Assay of Glutathione Reductase. In: Bergmeyen HV, editor(s). Methods of enzymatic analysis, 3rd ed. Vol. 3. Deerfield Beach, Fl: Verlog Chemie, 1983:258–65.Search in Google Scholar

[31] Mannervik B, Guthenberg C. Glutathione-S-transferase (human placenta). Methods Enzymol 1981;77:231–5.10.1016/S0076-6879(81)77030-7Search in Google Scholar

[32] Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121–6.10.1016/S0076-6879(84)05016-3Search in Google Scholar

[33] Moshage H, Kok B, Huizenga JR, Hansen PL. Nitrite and nitrate determinations in plasma: a critical evaluation. Clin Chem 1995;41:892–6.10.1093/clinchem/41.6.892Search in Google Scholar

[34] Okhawa H, Ohishi N, Yagi K. Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8.10.1016/0003-2697(79)90738-3Search in Google Scholar

[35] Samojlik I, Mijatović V, Petković S, Skrbić B, Božin B. The influence of essential oil of aniseed (Pimpinella anisum, L.) on drug effects on the central nervous system. Fitoterapia 2012;83:1466–73.10.1016/j.fitote.2012.08.012Search in Google Scholar PubMed

[36] Shahamat Z, Abbasi-Maleki S, Mohammadi Motamed S. Evaluation of antidepressant-like effects of aqueous and ethanolic extracts of Pimpinella anisum fruit in mice. Avicenna J Phytomed 2016;6:322–8.Search in Google Scholar

[37] Deng XY, Xue JS, Li HY, Ma ZQ, Fu Q, Qu R. et al. Geraniol produces antidepressant-like effects in a chronic unpredictable mild stress mice model. Physiol Behav 2015;152 (Pt A):264–71.10.1016/j.physbeh.2015.10.008Search in Google Scholar PubMed

[38] Lohani A, Mishra AK, Verma A. Cosmeceutical potential of geranium and calendula essential oil: determination of antioxidant activity and in vitro sun protection factor. J Cosmet Dermatol 2019;18:550–7. DOI:10.1111/jocd.12789.Search in Google Scholar PubMed

[39] Giongo JL, de Almeida Vaucher R, Sagrillo MR, Vianna Santos RC, Duarte MM, Rech VC. et al. Anti-inflammatory effect of geranium nanoemulsion macrophages induced with soluble protein of Candida albicans. Microb Pathog 2017;110:694–702.10.1016/j.micpath.2017.01.056Search in Google Scholar PubMed

[40] Kobayashi Y, Sato H, Yorita M, Nakayama H, Miyazato H, Sugimoto K. et al. Inhibitory effects of geranium essential oil and its major component, citronellol, on degranulation and cytokine production by mast cells. Biosci Biotechnol Biochem 2016;80:1172–8.10.1080/09168451.2016.1148573Search in Google Scholar PubMed

[41] Ben Slima A, Ali MB, Barkallah M, Traore AI, Boudawara T, Allouche N. et al. Antioxidant properties of Pelargonium graveolens L’Her essential oil on the reproductive damage induced by deltamethrin in mice as compared to alpha-tocopherol. Lipids Health Dis 2013;12:30.10.1186/1476-511X-12-30Search in Google Scholar PubMed PubMed Central

[42] Yu C, Wei J, Yang C, Yang Z, Yang W, Jiang S. Effects of star anise (Illicium verum Hook.f.) essential oil on laying performance and antioxidant status of laying hens. Poult Sci 2018;97:3957–66.10.3382/ps/pey263Search in Google Scholar PubMed

[43] Huang Z, Liu X, Jia S, Zhang L, Luo Y. The effect of essential oils on microbial composition and quality of grass carp (Ctenopharyngodon idellus) fillets during chilled storage. Int J Food Microbiol 2018;266:52–9.10.1016/j.ijfoodmicro.2017.11.003Search in Google Scholar PubMed

[44] Kang P, Kim KY, Lee HS, Min SS, Seol GH. Anti-inflammatory effects of anethole in lipopolysaccharide-induced acute lung injury in mice. Life Sci 2013;93:955–61.10.1016/j.lfs.2013.10.014Search in Google Scholar

Received: 2019-02-01
Accepted: 2019-07-16
Published Online: 2019-09-06

© 2019 Walter de Gruyter GmbH, Berlin/Boston

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