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Licensed Unlicensed Requires Authentication Published by De Gruyter December 25, 2020

10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies

Kiptiyah Kiptiyah, Widodo Widodo, Gatot Ciptadi, Aulanni’am Aulanni’Am, Mohammad A. Widodo and Sutiman B. Sumitro



We investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells.


For the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20 µM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentrations, with incubation periods of 24, 48, 72, and 96 h. The obtained results were confirmed by in-silico studies.


The in-vitro data revealed that SOD activity and MDA concentration increased with increasing incubation periods: SOD activity at 0 µM (1.39 ± 0.24i), 12 µM (16.42 ± 0.35ab), 16 µM (17.28 ± 0.55ab), 20 µM (17.81 ± 0.12a), with a contribution of 71.1%. MDA concentration at 0 µM (17.82 ± 1.39 l), 12 µM (72.99 ± 0.31c), 16 µM (79.77 ± 4.19b), 20 µM (85.07 ± 2.57a), with a contribution of 73.1%. Based on this, the in-silico data uncovered that 10˗gingerol induces oxidative stress in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.


10-gingerol induces oxidative stress in cumulus cells through enhancing SOD activity and MDA concentration by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.





chem identity


enzyme-linked immunoabsorbent assay


endothelial nitric oxide synthase


forkhead box


glycogen synthase kynase-3 β




5-hydroxytryptamine receptor 1 A


integrin-linked kinase


medium 199




murine double minute clone 2


mammalian target of rapamycin


nitric oxide


nitric oxide synthase


nitric oxide synthase 3


protein data bank


phosphatidylinositol-3-OH kinase/AKT


phosphatase and tensin homologue delete on chromosome ten


polyunsaturated fatty acids


rapamycin-insensitive companion of mTOR


reactive oxygen species


superoxide dismutase.


We would like to thank the laboratories in the Maulana Malik Ibrahim Islamic State University of Malang, State Polytechnic of Malang, Brawijaya University of Malang, the abattoir in Malang City, and CV. Gamma Scientific Biolab of Malang, who collaborated with us and National Educational Ministry of Indonesian Republic Government which supplied fund No. 708/H10.14/AK/2010. The authors declare that no conflict of interest would prejudice the impartiality of this scientific work.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: National Educational Ministry of Indonesian Republic Government, fund No. 708/H10.14/AK/2010.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


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Received: 2019-03-01
Accepted: 2019-10-24
Published Online: 2020-12-25

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