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Licensed Unlicensed Requires Authentication Published by De Gruyter December 25, 2020

Identification of bioactive constituents in Coldenia procumbens L. and its antidiabetic activity against streptozotocin induced Wistar albino rats

  • Ganesan Rethinam EMAIL logo and Mathuram Venkatanarasimhan



Type II diabetes, a multifactorial progressive disorder is the prime concern of the twenty-first century. Modern medicine is proven effective in delaying the effects of diabetes. However, the side effects are amplified over time. In order to find relief from side effects, people are rigorously searching for alternative treatment.


In this study, we aim to identify the bioactive components in the Coldenia procumbens L. and assess its anti-diabetic effect.

Materials and Methods

Initially, the plant was extracted using chloroform and methanol. Both the extracts were analysed using IR Spectrum and NMR. The methanol extract of Coldenia procumbens L. was assessed for its anti-hyperglycaemic activity against streptozotocin induced animals.


The IR spectrum of the extracts was compared with standard compounds and four compounds, α-amyrin, β-sitosterol, β-stigmasterol and wedelolactone was identified. Methanol extract of Coldenia procumbens L. decreased glucose levels in serum and enzymes levels. Histopathology of pancreas showed excellent recovery from the damage induced by streptozotocin.


The compounds identified in Coldenia procumbens L. have significant anti-diabetic, insulin mimetic and insulin secretory activities with their complete mechanisms already studied in detail. Also, Coldenia procumbens L. methanol extract showed significant anti-hyperglycaemic activity. The plant should be further studied to be developed as an alternative medicine.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


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Received: 2019-04-06
Accepted: 2019-10-14
Published Online: 2020-12-25

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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