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Licensed Unlicensed Requires Authentication Published by De Gruyter September 17, 2019

In vivo neuroprotective, antinociceptive, anti-inflammatory potential in Swiss albino mice and in vitro antioxidant and clot lysis activities of fractionated Holigarna longifolia Roxb. bark extract

  • Md. Zia Uddin , Md. Sohel Rana , Shahadat Hossain , Sumaiya Ferdous , Emon Dutta , Mycal Dutta and Talha Bin Emran ORCID logo EMAIL logo



The study investigated the in vivo neuroprotective, antinociceptive, anti-inflammatory potential and in vitro antioxidant and clot lysis activities of crude methanol extract with its different solvent-soluble fractions like petroleum ether (PESF), carbon tetrachloride (CTSF), chloroform (CSF) and aqueous (AQSF) of Holigarna longifolia Roxb.


Phenobarbitone-induced sleeping time method was used for the neuroprotective activity, writhing response experimental model introduced by acetic acid was designed for antinociceptive efficacy, carrageenan-induced paw edema model was carried out for anti-inflammatory activity, DPPH free radical scavenging activity was assessed for antioxidant activity and clot lysis model was investigated for the thrombolytic potential of the plant.


On investigation it was found that methanol extract and CS fraction revealed statistically meaningful (p<0.05) neuroprotective activity by increasing phenobarbitone-induced sleeping time of mice, produced substantial (p<0.05) inflammation inhibitory efficacy compared to standard diclofenac sodium and also exhibited statistically significant (p<0.01) oxidative stress inhibitory efficacy by inhibiting free radical formation compared to ascorbic acid as standard. Only methanol extract produced significant (p<0.05) antinociceptive activity by inhibiting abdominal writhes produced by acetic acid compared to standard analgesic drug diclofenac sodium. And only aqueous soluble fraction exhibited moderate clot lysis activity compared to streptokinase as standard.


The findings demonstrate that H. longifolia could be potential neuroprotective due to its justified antioxidative capacity as well as clot lysis properties.

Correction note

Correction added after online publication September 17, 2019: Mistakenly this article was already published ahead of print under the title “In vivoneuroprotective, antinociceptive, anti-inflammatory potential in Swiss albino mice and in vitro antioxidant and clot lysis activities of fractionated Holigrana longifolia Roxb. leaves extract”


The authors are grateful to the Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh and Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chattogram-4381, Bangladesh for providing necessary facilities for this research work. Authors are also thankful to Prof. Dr. S. K. S. Hazari, Vice-Chancellor, BGC Trust University Bangladesh, for providing research facilities to conduct the research at the Department of Pharmacy, BGC Trust University Bangladesh.

  1. Author contributions: This work was carried out in collaboration between all authors. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved the submission. Authors MZU, SH, SF, ED, and MD performed experiments. Authors MZU, SF, ED, and MD collected the plant barks and prepared the extracts and fractions. MZU, SF, and ED performed the antidepressant, analgesic and anti-inflammatory activity. MZU and TBE performed the antioxidant and clot lysis activity. MZU, MSR, SH, and TBE performed statistical analysis. MZU, MSR, SH, and TBE conceived the study and designed the experimental procedures. MSR, SH, and TBE designed and planned the studies, supervised the experiments. TBE also acted for all correspondences. MZU, MSR, SH, and TBE participated in the manuscript draft and has thoroughly checked and revised the manuscript for necessary changes in format, grammar, and English standard. All authors read and approved the final version of the manuscript. All authors read and approved the manuscript.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.


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This work was done in partial fulfillment of the Ph.D. thesis of MZU submitted to Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

Received: 2019-04-14
Accepted: 2019-05-15
Published Online: 2019-09-17

© 2019 Walter de Gruyter GmbH, Berlin/Boston

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