Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter September 17, 2019

In vivo neuroprotective, antinociceptive, anti-inflammatory potential in Swiss albino mice and in vitro antioxidant and clot lysis activities of fractionated Holigarna longifolia Roxb. bark extract

  • Md. Zia Uddin , Md. Sohel Rana , Shahadat Hossain , Sumaiya Ferdous , Emon Dutta , Mycal Dutta and Talha Bin Emran ORCID logo EMAIL logo

Abstract

Background

The study investigated the in vivo neuroprotective, antinociceptive, anti-inflammatory potential and in vitro antioxidant and clot lysis activities of crude methanol extract with its different solvent-soluble fractions like petroleum ether (PESF), carbon tetrachloride (CTSF), chloroform (CSF) and aqueous (AQSF) of Holigarna longifolia Roxb.

Methods

Phenobarbitone-induced sleeping time method was used for the neuroprotective activity, writhing response experimental model introduced by acetic acid was designed for antinociceptive efficacy, carrageenan-induced paw edema model was carried out for anti-inflammatory activity, DPPH free radical scavenging activity was assessed for antioxidant activity and clot lysis model was investigated for the thrombolytic potential of the plant.

Results

On investigation it was found that methanol extract and CS fraction revealed statistically meaningful (p<0.05) neuroprotective activity by increasing phenobarbitone-induced sleeping time of mice, produced substantial (p<0.05) inflammation inhibitory efficacy compared to standard diclofenac sodium and also exhibited statistically significant (p<0.01) oxidative stress inhibitory efficacy by inhibiting free radical formation compared to ascorbic acid as standard. Only methanol extract produced significant (p<0.05) antinociceptive activity by inhibiting abdominal writhes produced by acetic acid compared to standard analgesic drug diclofenac sodium. And only aqueous soluble fraction exhibited moderate clot lysis activity compared to streptokinase as standard.

Conclusion

The findings demonstrate that H. longifolia could be potential neuroprotective due to its justified antioxidative capacity as well as clot lysis properties.


Correction note

Correction added after online publication September 17, 2019: Mistakenly this article was already published ahead of print under the title “In vivoneuroprotective, antinociceptive, anti-inflammatory potential in Swiss albino mice and in vitro antioxidant and clot lysis activities of fractionated Holigrana longifolia Roxb. leaves extract”


Acknowledgments

The authors are grateful to the Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh and Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chattogram-4381, Bangladesh for providing necessary facilities for this research work. Authors are also thankful to Prof. Dr. S. K. S. Hazari, Vice-Chancellor, BGC Trust University Bangladesh, for providing research facilities to conduct the research at the Department of Pharmacy, BGC Trust University Bangladesh.

  1. Author contributions: This work was carried out in collaboration between all authors. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved the submission. Authors MZU, SH, SF, ED, and MD performed experiments. Authors MZU, SF, ED, and MD collected the plant barks and prepared the extracts and fractions. MZU, SF, and ED performed the antidepressant, analgesic and anti-inflammatory activity. MZU and TBE performed the antioxidant and clot lysis activity. MZU, MSR, SH, and TBE performed statistical analysis. MZU, MSR, SH, and TBE conceived the study and designed the experimental procedures. MSR, SH, and TBE designed and planned the studies, supervised the experiments. TBE also acted for all correspondences. MZU, MSR, SH, and TBE participated in the manuscript draft and has thoroughly checked and revised the manuscript for necessary changes in format, grammar, and English standard. All authors read and approved the final version of the manuscript. All authors read and approved the manuscript.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

[1] Barrett JE. Animal models in psychopharmacology (Olivier, B., Mos, J. and Slangen, J.L.). Switzerland (CH): Basel Birkhäuser. Animal behavior models in the analysis and understanding of anxiolytic drugs acting at serotonin receptors, 1991:37–52.10.1007/978-3-0348-6419-0_3Search in Google Scholar

[2] Kim Y, Cheol Oh H, Park JW, Kim IS, Kim JY, Kim KC, et al. Diagnosis and treatment of inflammatory joint disease. Hip Pelvis 2017;29:211–22.10.5371/hp.2017.29.4.211Search in Google Scholar

[3] Chatterje GK, Pal SP. Search for anti-inflammatory agents from Indian medicinal plants. Indian Drugs 1984;21:413.Search in Google Scholar

[4] Thomas MC. Diuretics, ACE inhibitors and NSAIDs – the triple whammy. Medical J Australia 2000;172:184–5.10.5694/j.1326-5377.2000.tb125548.xSearch in Google Scholar

[5] Ahmad F, Khan RA, Rasheed S. Study of analgesic and anti-inflammatory activity from plant extracts of Lactuca scariola and Artemisia absinthium. J Islamic Acad Sci 1992;5:111–14.Search in Google Scholar

[6] Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence. Lancet 1994;344:721–4.10.1016/S0140-6736(94)92211-XSearch in Google Scholar

[7] Kuhnan J. The flavonoids. A class of semi-essential food components; their role in human nutrition. World Rev Nutr Diet 1976;24:117–91.10.1159/000399407Search in Google Scholar

[8] Kumpulainen JT, Salonen JT. Natural antioxidants and anticarcinogens in nutrition, health and disease. UK: The Royal Society of Chemistry, 1999:178–87.10.1533/9781845698409Search in Google Scholar

[9] Younes M. Inhibitory action of some flavonoids on enhanced spontaneous lipid peroxidation following glutathione depletion. Planta Med 1981;43:240–5.10.1055/s-2007-971503Search in Google Scholar

[10] Schuler P. Natural antioxidants exploited commercially. In: BJ Hudson, editor. Food Antioxidants. London: Elsevier, 1990:99–170.10.1007/978-94-009-0753-9_4Search in Google Scholar

[11] Chu Y. Flavonoid content of several vegetables and their antioxidant activity. J Sci Food Agricul 2000;80:561–6.10.1002/(SICI)1097-0010(200004)80:5<561::AID-JSFA574>3.0.CO;2-#Search in Google Scholar

[12] Koleva II, Van Beek TA, Linssen JP, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 2002;13:8–17.10.1002/pca.611Search in Google Scholar

[13] Mantle D, Eddeb F, Pickering AT. Comparison of relative antioxidant activities of British medicinal plant species in vitro. J Ethnopharmacol 2000;72:47–51.10.1016/S0378-8741(00)00199-9Search in Google Scholar

[14] Oke JM, Hamburger MO. Screening of some Nigerian medicinal plants for antioxidant activity using 2, 2-diphenyl-picryl-hydrazyl radical. African J Biomed Res 2002;5:77–9.Search in Google Scholar

[15] Haines ST, Bussey HI. Thrombosis and the pharmacology of antithrombotic agents. Ann Pharmacother 1995;29:892–904.10.1177/106002809502900912Search in Google Scholar

[16] Patel SC, Mody A. Cerebral hemorrhagic complications of thrombolytic therapy. Prog Cardiovasc Dis 1991;42:217–33.10.1016/S0033-0620(99)70004-6Search in Google Scholar

[17] Uddin SN. Traditional uses of ethnomedicinal plants of the Chittagong hill tracts. Bangladesh: Bangladesh National Herbarium, 2006:604–604.Search in Google Scholar

[18] Muhit MA, Tareq SM, Apu AS, Basak D, Islam MS. Isolation and identification of compounds from the leaf extract of Dillenia indica Linn. Bangladesh Pharm J 2010;13:49–53.Search in Google Scholar

[19] Williamson EM, Okpako DT, Evans FJ. Pharmacological methods in phytotherapy research: selection, preparation and pharmacological evaluation of plant material, 1 ed. England: John Willey & Sons, 1996:18–32.Search in Google Scholar

[20] Koster R, Anderson M, Beer EJ. Acetic acid for analgesic screening. Federation Proceeds 1959;18:412–16.Search in Google Scholar

[21] Winter CA, Risley EA, Nuss CW. Carrageenan induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med 1962;111:544–7.10.3181/00379727-111-27849Search in Google Scholar

[22] Prasad S, Rajpal SK, Jayant YD, Hemant JP, Girdhar MT, Hatim FD. Development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thrombosis J 2006;4:14.10.1186/1477-9560-4-14Search in Google Scholar

[23] Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebenson Wiss Technol 1995;28:25–30.10.1016/S0023-6438(95)80008-5Search in Google Scholar

[24] Ratnasooriya WD, Fernando TS, Madubashini PP. In vitro thrombolytic activity of Sri Lankan black tea, Camellia sinensis (L.) O. Kuntze. J Nat Sci Found Sri Lanka 2008;36:179–81.10.4038/jnsfsr.v36i2.151Search in Google Scholar

[25] Sakiyama Y, Sujaku T, Furuta A. A novel automated method for measuring the effect of analgesics on formalin-evoked licking behavior in rats. J Neurosci Methods 2008;167:167–75.10.1016/j.jneumeth.2007.08.003Search in Google Scholar

[26] Adzu B, Amos S, Kapu S, Gamaniel K. Anti-inflammatory and anti-nociceptive effects of Sphaeranthus senegalensis. J Ethnopharmacol 2003;84:169–73.10.1016/S0378-8741(02)00295-7Search in Google Scholar

[27] Middleton E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev 2000;52:673–751.Search in Google Scholar

[28] Uche F, Aprioku J. The phytochemical constituents, analgesic and anti-inflammatory effects of methanol extract of Jatropha curcas leaves in mice and Wister albino rats. Appl Sci Environ Manage 2008;12:128–31.10.4314/jasem.v12i4.55247Search in Google Scholar

[29] Uddin MM, Ahmed S, Kabir MS, Rahman MS, Sultan RA, Emran TB. In vivo analgesic, anti-inflammatory potential in Swiss albino mice and in vitro thrombolytic activity of hydroalcoholic fruits extract from Daemonorops robusta Warb. J Appl Pharm Sci 2017;7:104–13.10.7324/JAPS.2017.70114Search in Google Scholar

[30] Chen YF, Tsai HY, Wu TS. Anti-inflammatory and analgesic activities from roots of Angelica pubescens. Planta Med 1995;61:2–8.10.1055/s-2006-957987Search in Google Scholar

[31] Roumestan C, Michel A, Bichon F, Portet K, Detoc M, Henriquet C, et al. Anti-inflammatory properties of desipramine and fluoxetine. Respir Res 2007;8:35.10.1186/1465-9921-8-35Search in Google Scholar

[32] Damas J, Remacle-Volon G, Deflandre E. Further studies of the mechanism of counter irritation by turpentine. Naunyn-Schmiedeberg’s Arch Pharmacol 1986;332:196–200.10.1007/BF00511412Search in Google Scholar

[33] Dawson J, Sedgwick A, Edwards J, Lees P. A comparative study of the cellular, exudative and histological responses to carrageenan, dextran and zymosan in the mouse. Int J Tissue React 1991;13:171–85.Search in Google Scholar

[34] Dudhgaonkar SP, Tandan SK, Bhat AS, Jadhav SH, Kumar D. Synergistic anti-inflammatory interaction between meloxicam and aminoguanidine hydrochloride in carrageenan-induced acute inflammation in rats. Life Sci 2006;78:1044–8.10.1016/j.lfs.2005.06.002Search in Google Scholar

[35] Vinegar R, Schreiber W, Hugo R. Biphasic development of carrageenin edema in rats. J Pharmacol Exp Ther 1969;166:96–103.Search in Google Scholar

[36] Marí M, Colell A, Morales A, von Montfort C, Garcia-Ruiz C, Fernández-Checa JC. Redox control of liver function in health and disease. Antioxid Redox Signal 2010;12:1295–331.10.1089/ars.2009.2634Search in Google Scholar

[37] Domitrovic R, Potocnjak I. A comprehensive overview of hepatoprotective natural compounds: mechanism of action and clinical perspectives. Arch Toxicol 2016;90:39–79.10.1007/s00204-015-1580-zSearch in Google Scholar

[38] Parejo I, Viladomat F, Bastida J, Rosas-Romero A, Flerlage N, Burillo J, et al. Comparison between the radical scavenging activity and antioxidant activity of six distilled and non-distilled Mediterranean herbs and aromatic plants. J Agric Food Chem 2002;50:6882–90.10.1021/jf020540aSearch in Google Scholar

[39] Parry MA, Zhang XC, Bode W. Molecular mechanisms of plasminogen activation: bacterial cofactors provide clues. Trends Biochem Sci 2000;25:53–9.10.1016/S0968-0004(99)01521-2Search in Google Scholar

[40] Collen D. Coronary thrombolysis: streptokinase or recombinant tissue-type plasminogen activator? Ann Intern Med 1990;112:529–38.10.7326/0003-4819-112-7-529Search in Google Scholar PubMed

[41] Mahmud S, Akhter S, Rahman MA, Aklima J, Merry SR, Jubair SM, et al. Antithrombotic effects of five organic extracts of Bangladeshi plants in vitro and mechanisms in in silico models. Evid Based Complement Alternat Med 2015;782742:17.10.1155/2015/782742Search in Google Scholar

[42] Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Int Pharmaceutica Sciencia 2011;1:98–106.Search in Google Scholar


Note

This work was done in partial fulfillment of the Ph.D. thesis of MZU submitted to Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.


Received: 2019-04-14
Accepted: 2019-05-15
Published Online: 2019-09-17

© 2019 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 1.3.2024 from https://www.degruyter.com/document/doi/10.1515/jcim-2019-0102/html
Scroll to top button