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Licensed Unlicensed Requires Authentication Published by De Gruyter September 18, 2019

Virgin coconut oil supplementation in diet modulates immunity mediated through survival signaling pathways in rats

  • Lalgi Hima , Uday P Pratap , Sunil Karrunanithi , Kishore A. Ravichandran , Ramasamy Vasantharekha and Srinivasan ThyagaRajan EMAIL logo

Abstract

Background

Virgin coconut oil (VCO), a cold processed form of coconut oil, is traditionally consumed in Asian countries owing to its nutritional and medicinal properties. The aim of this study was to investigate whether the health benefits of VCO involve alterations in immune responses that are regulated by intracellular signaling molecules in the spleens of rats.

Methods

Young male Wistar rats were fed with three doses of VCO in diet for 30 days. At the end of the treatment period, spleens were isolated and in vitro effects on immune responses (Concanavalin A [Con A]-induced lymphoproliferation and cytokine production), and direct effects of VCO treatment on intracellular signaling molecules and antioxidant status were examined. Serum was collected to measure glucose, lipid levels, and leptin.

Results

VCO supplementation in diet enhanced Con A-induced splenocyte proliferation and Th1 cytokine production while it suppressed the proinflammatory cytokine production. VCO increased the expression of mechanistic target of rapamycin (p-mTOR), sirtuin1 (SIRT1), liver kinase B1 (p-LKB1) p-ERK, and p-CREB in spleen. Similarly, VCO increased the activities of antioxidant enzymes while it suppressed lipid peroxidation in the spleen. VCO diet had hypolipidemic effects on the rats: an increase in high density lipoprotein cholesterol (HDL-C) levels while lowering triacylglycerol (TAG) levels.

Conclusion

The health benefits of VCO may be mediated through enhanced Th1 immunity through the upregulation of survival signaling pathways and inhibition of free radical generation in the spleen besides its capacity to induce hypolipidemia.

Acknowledgments

Authors acknowledge Mr Kannan Thangamani, Mr Chockalingam Ramanathan, Mr Murali Mahadevan Hariharan, Ms Shaili Gour, Mr Mantavya Patel, Ms Supraja Prabakar, Ms Sahana Jayakumar, Ms Aanal Bhatt, Mr Nishant Bishnoi, and Mr Smit Mahajani for their technical assistance.

  1. Author Contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research Funding: This research is funded by Coconut Development Board, Ministry of Agriculture, Govt. of India (F.No. 1345/2013 Tech). Lalgi Hima is the recipient of fellowship from the grant. The Coconut Development Board had no role in design, analysis or writing of this article.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2019-04-23
Accepted: 2019-05-30
Published Online: 2019-09-18

© 2019 Walter de Gruyter GmbH, Berlin/Boston

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