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Licensed Unlicensed Requires Authentication Published by De Gruyter September 18, 2019

Crocin treatment decreased pancreatic atrophy, LOX-1 and RAGE mRNA expression of pancreas tissue in cholesterol-fed and streptozotocin-induced diabetic rats

  • Mohammad Ehsan Bayatpoor , Saeed Mirzaee , Mohammad Karami Abd , Mohammad Taghi Mohammadi , Shima Shahyad , Zahra Bahari ORCID logo EMAIL logo and Javad Raouf Sarshoori



Oxidative stress in diabetic mellitus is a consequence of oxidative stress, which plays a critical role in the pathogenesis of diabetic tissue damage. Receptors for advanced glycation end products and for oxidized low-density lipoproteins (LDL) have critical contribution in oxidative tissue damage. The present study investigated whether anti-diabetic effects of Crocin via modulation of mRNA expression of RAGE and LOX-1 receptors in diabetic rats.


In the current study, high-fat cholesterol (HFC) and streptozotocin (40 mg/kg) used to induce type II diabetes. Experimental groups as follows: (Group 1: control); (Group 2: control treatment [Crocin]); (Group 3: DM [STZ]); (Group 4: DM treatment [STZ + Crocin]); (Group 5; DM + HFC [STZ + HFC]); (Group 6; DM + HFC treatment [STZ + HFC + Crocin]). Crocin (20 mg/kg/day, i.p.) administered in treatment groups for 60 days. Serum glucose and cholesterol levels evaluated on days 5, 30 and 60 after induction of DM. Pancreatic tissue from all group removed on day 60 for histological and RT-PCR analysis.


Application of Crocin significantly decreased serum cholesterol levels on day 60 after induction of DM in diabetic + HFC rats. Moreover, Crocin significantly decreased serum glucose levels on days 30 and 60 both in diabetic and diabetic + HFC rats. Crocin partially prevented the atrophic effects of STZ on both exocrine and endocrine parts of pancreas. Additionally, Crocin significantly decreased LOX-1 and RAGE mRNA expression OF pancreas in diabetic rats.


The current study suggested that Crocin suppressed atrophic change of the pancreas by decrease of LOX-1 and RAGE mRNA expression in diabetic rats.

List of abbreviation


Diabetes Mellitus


reactive oxygen spacious


advanced glycation end-products


AGE-specific receptor


low-density lipoproteins


oxidized LDL


lectin-like oxLDL receptor-1




high-fat cholesterol


reverse transcriptase-polymerase chain reaction


Complementary DNA

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This study was supported by a grant from Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

  3. Employment or leadership: None declared.

  4. Honorarium: None declare.

  5. Competing interests: None declare.


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Received: 2019-04-24
Accepted: 2019-07-14
Published Online: 2019-09-18

© 2019 Walter de Gruyter GmbH, Berlin/Boston

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