Ghee is widely considered as the Indian name for clarified butterfat and processing of ghee with therapeutic herbs i.e., ghrita is renowned for augmenting their medicinal properties. The wound is considered as a challenging clinical problem with early and late complications. To reduce the burden of wounds with the shortest period and minimum scaring, an attempt was made to prepare and evaluate the wound healing potential of ghee based polyherbal formulation.
Based on local ethnic tribal claims, Semecarpus anacardium L., Argemone mexicana L., Cocculus hirsutus L., and Woodfordia fruticosa K. were collected from Western Ghats of India. The polyherbal Bhallatakadi Ghrita (BG) formulation was prepared as per Ayurvedic procedure and assessed for its wound healing potential using incision and excision wound animal models.
BG treated group showed a complete contraction of wounds (99.82 ± 0.10%) (p<0.001) with 15.17 ± 0.40 days re-epithelization time and breaking strength (531.50 ± 5.89) (p<0.05). The hydroxyproline content of BG was found to be significantly higher i.e., 4.23 ± 0.21 (p<0.05). Quantitative estimation of BG exhibited 54.7 ± 3.7 mg100 g−1 of polyphenols and 42.3 ± 5.4 mg.100 g−1 flavonoids in terms of gallic acid and quercetin respectively. HPLC analysis revealed the presence of gallic acid and quercetin whereas the presence of fatty acids was confirmed by GC-MS analysis.
It may conclude that the presence of quercetin, gallic acid, and fatty acids could have accelerated the healing rate of the ghrita formulation, as they have already been known for their potential wound healing properties.
The authors would like to acknowledge Chaitanya laboratory, Pune for histological study and Central Instrumentation Facility, SPPU, Pune for analytical study.
Research funding: None declared.
Author contributions: Both the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Ethical approval: The research related to the animals use has been compiled with the relevant national regulations and institutional policies for the care and use of animals.
1. Dwivedi, D, Dwivedi, M, Malviya, S, Singh, V. Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. J Tradit Complementary Med 2017;7:79–85. https://doi.org/10.1016/j.jtcme.2015.12.002.Search in Google Scholar
2. Velnar, T, Bailey, T, Smrkolj, V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res 2009;37:1528–42. https://doi.org/10.1177/147323000903700531.Search in Google Scholar
6. Sathish, HS, Jyothi, T, Ashok, BK, Vaghela, DB, Bhuyan, C, Ravishankar, B. Wound healing activity of Jati kalpa ghrita in albino rats. Int J Ayu Allied Sci 2013;2:242–7.Search in Google Scholar
7. Choudhary, N, Soni, P, Swarnkar, M. A randomized controlled clinical study of karanjadhya ghrita in the management of dushta vrana (Chronic wounds). Int J Ayurvedic Herb Med 2015;5:1745–53.Search in Google Scholar
10. Babu, B, Ravi, M, Kumar, AB, Sudheendra, VH, Ravishankar, B. Burn wound healing potential of Plain gritha, Shatadhauta ghrita and Sahasradhauta ghrita on wistar albino rats. Indian J Tradit Knowl 2015;14:273–8.Search in Google Scholar
11. Waghmare, D, Dharne, V, Kashikar, S, Yadav, S. A comparative study of effect of Yashtimadhu ghrita and Tikdadi ghrita locally in the management of post operative fistulectomy wound. Int J Ayurvedic Herb Med 2016;6:2359–65.Search in Google Scholar
12. Wayal, SR, Gurav, SS. Bhallatakadi ghrita: development and evaluation with reference to murcchana and shata-dhauta process. J Ayurveda Integr Med 2020;11:261–9.10.1016/j.jaim.2020.05.005Search in Google Scholar
13. Wayal, SR, Gurav, SS. Pharmacognostic and phytochemical investigation of potentially important plants of Western Ghats, India. Int J Pharm Sci Res 2019;10:3101–8.Search in Google Scholar
14. Lingaraju, GM, Krishna, V, Joy Hoskeri, H, Pradeepa, K, Venkatesh Babu, PS. Wound healing promoting activity of stem bark extract of Semecarpus anacardium using rats. Nat Prod Res 2012;26:2344–7. https://doi.org/10.1080/14786419.2012.656108.Search in Google Scholar
15. Das, GK, Murthy, PN. Evaluation of Argemone mexicana Linn leaves for wound healing activity. J Nat Prod Plant Resour 2011;1:46–56.Search in Google Scholar
16. Ayyanar, M, Ignacimuthu, S. Traditional knowledge of Kani tribals in Kouthalai of Tirunelveli hills, Tamil Nadu, India. J Ethnopharmacol 2005;102:246–55. https://doi.org/10.1016/j.jep.2005.06.020.Search in Google Scholar
17. Seethapathy, GS, Ravikumar, K, Paulsen, BS, de Boer, HJ, Wangensteen, H. Ethnobotany of dioecious species: traditional knowledge on dioecious plants in India. J Ethnopharmacol 2018;221:56–64. https://doi.org/10.1016/j.jep.2018.04.011.Search in Google Scholar
18. Kalirajan, A, Savarimuthu, MJ, Ranjitsingh, AJ, Padmalatha, C. Antimicrobial and wound healing studies on the extracts of the medicinal plant Cocculus hirsutus (Linn). Int J Appl Biol Pharma 2012;3:63–7.Search in Google Scholar
19. Verma, N, Amresh, G, Sahu, PH, Mishra, N, Rao, CV, Singh, AP. Wound healing potential of flowers extracts of Woodfordia fruticosa Kurz. Indian J Biochem Biophys 2013;50:296–304.Search in Google Scholar
20. Das, PK, Goswami, S, Chinniah, A, Panda, N, Banerjee, S, Sahu, N. Woodfordia fruticosa: traditional uses and recent findings. J Ethnopharmacol 2007;110:189–99. https://doi.org/10.1016/j.jep.2006.12.029.Search in Google Scholar
21. Gurav, SS, Tilloo, SK, Burade, KB. Histological and histochemical staining techniques. In: Gurav, S, Gurav, N, editors. Indian herbal drug microscopy. New York: Springer Sciences; 2014:9–14 pp.10.1007/978-1-4614-9515-4_3Search in Google Scholar
22. Gurav, S, Gurav, N. Herbal drug microscopy. In: Gurav, S, Gurav, N, editors. Indian herbal drug microscopy. New York: Springer Sciences; 2014:186–7 pp.10.1007/978-1-4614-9515-4_4Search in Google Scholar
23. Gurav, S, Gurav, N. Sectioning methods. In: Gurav, S, Gurav, N, editors. Indian herbal drug microscopy. New York: Springer Sciences; 2014:5–7 pp.10.1007/978-1-4614-9515-4_2Search in Google Scholar
24. Lalsare, S, Verma, PK, Khatak, M, Ranjan, S, Rajurakar, S, Gurav, SS. Anti-inflammatory and antimicrobial activity of Flacourtia ramontchi leaves. Int J Drug Dev Res 2011;3:308–13.Search in Google Scholar
25. Gurav, SS, Deshkar, NS, Tilloo, SK, Duragkar, NJ, Burade, KB. Antimicrobial and antioxidant evaluation of Flacourtia ramontchi L. Herit. J Herbs, Spices, Med Plants 2013;19:76–95. https://doi.org/10.1080/10496475.2012.743107.Search in Google Scholar
26. Gurav, SS, Gurav, NS, Patil, AT, Duragkar, NJ. Effect of explant source, culture media, and growth regulators on callogenesis and expression of secondary metabolites of Curcuma longa. J Herbs, Spices, Med Plants 2020;26:172–90. https://doi.org/10.1080/10496475.2019.1689542.Search in Google Scholar
27. Gurav, S, Deshkar, N, Gulkari, V, Duragka, N, Pati, A. Free radical scavenging activity of Polygala chinensis Linn. Pharmacologyonline 2007;2:245–53.Search in Google Scholar
28. Mo, J, Panichayupakaranant, P, Kaewnopparat, N, Nitiruangjaras, A, Reanmongkol, W. Wound healing activities of standardized pomegranate rind extract and its major antioxidant ellagic acid in rat dermal wounds. J Nat Med 2014;68:377–86. https://doi.org/10.1007/s11418-013-0813-9.Search in Google Scholar
29. Gurav, S, Gulkari, V, Duragkar, N, Sakharwade, S, Itankar, P, Patil, A. Analgesic and anti-inflammatory activity of Flacourtia ramontchi L. Herit. Pharmacologyonline 2007;2:20–31.Search in Google Scholar
30. OECD. OECD guideline for testing of chemicals. Available from: https://www.oecd.org/env/ehs/testing/2-TG 402_draft_26 Oct 2015.pdf [Assessed 30 Jul 2016].Search in Google Scholar
31. Birch, M, Tomlinson, A, Ferguson, MW. Animal models for adult dermal wound healing. In: Varga, J, Brenner, DA, Phan, SH, editors. Fibrosis research: methods and protocols. Totowa: Humana Press; 2005:223–35 pp.10.1385/1-59259-940-0:223Search in Google Scholar
32. Mukherjee, PK. Quality control of herbal drugs an approach to evaluation of botanicals. Delhi: Business Horizons Pharmaceutical Publishers; 2008.Search in Google Scholar
33. Nayak, BS, Sandiford, S, Maxwell, A. Evaluation of the wound-healing activity of ethanolic extract of Morinda citrifolia L. Leaf. Evid Base Compl Alternative Med 2009;6:351–6. https://doi.org/10.1093/ecam/nem127.Search in Google Scholar
34. Datta, HS, Mitra, SK, Patwardhan, B. Wound healing activity of topical application forms based on Ayurveda. Evid Base Compl Alternative Med 2011;134378:1–10. https://doi.org/10.1093/ecam/nep015.Search in Google Scholar
35. Ramachandra, YL, Gavimath, CC, Sudeep, HV, Ganapathy, PS, Rai, SP. Evaluation of wound healing activity of Butea Monosperma Lam extracts on rats. Pharmacologyonline 2009;2:203–16.Search in Google Scholar
36. Bahir, SS, Wayal, SR, Barke, SA, Shelke, TT, Gunjegaonkar, SM, Hadke, SP, et al.. Preliminary phytochemical investigation of Semecarpus anacardium Linn. Int J Pharm Pharmaceut Sci 2013;5:102–7.Search in Google Scholar
37. Esposito, D, Munafo, JP, Lucibello, T, Baldeon, M, Komarnytsky, S, Gianfagna, TJ. Steroidal glycosides from the bulbs of Easter lily (Lilium longiflorum Thunb.) promote dermal fibroblast migration in-vitro. J Ethnopharmacol 2013;148:433–40. https://doi.org/10.1016/j.jep.2013.04.032.Search in Google Scholar
38. Akdemir, Z, Kahraman, C, Tatlı, II, Kupeli Akkol, E, Süntar, I, Keles, H. Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum mucronatum Lam. J Ethnopharmacol 2011;136:436–43. https://doi.org/10.1016/j.jep.2010.05.059.Search in Google Scholar
39. Chaudhari, M, Mengi, S. Evaluation of phytoconstituents of Terminalia arjuna for wound healing activity in rats. Phytother Res 2006;20:799–805. https://doi.org/10.1002/ptr.1857.Search in Google Scholar
40. Juneja, K, Mishra, R, Chauhan, S, Gupta, S, Roy, P, Sircar, D. Metabolite profiling and wound-healing activity of Boerhavia diffusa leaf extracts using in vitro and in vivo models. J Tradit Complementary Med 2020;10:52–9. https://doi.org/10.1016/j.jtcme.2019.02.002.Search in Google Scholar
41. Hasanoglu, A, Ara, C, Ozen, S, Kali, K, Senol, M, Ertas, E. Efficacy of micronized flavonoid fraction in healing of clean and infected wounds. Int J Angiol 2001;10:41–4. https://doi.org/10.1007/BF01616343.Search in Google Scholar
42. Zyuz’kov, GN, Krapivin, AV, Nesterova, YV, Povetieva, TN, Zhdanov, VV, Suslov, NI, et al.. Mechanisms of regeneratory effects of baikal aconite diterpene alkaloids. Bull Exp Biol Med 2012;153:847–51.10.1007/s10517-012-1841-2Search in Google Scholar
43. Ferreira, AM, Rigotti, MA, Loureiro, MR. The use of fatty acids in wound care: an integrative review of the Brazilian literature. Rev Esc Enferm USP 2012;46:752–60. https://doi.org/10.1590/s0080-62342012000300030.Search in Google Scholar
44. Rex, JR, Nadar, MS, Paulraj, MS. Plant-derived compounds for wound healing- A review. Org Med Chem Int J 2018;5:1–5.Search in Google Scholar
45. Singh, MP, Gupta, A, Sisodia, SS. Wound healing activity of Terminalia bellerica Roxb. and gallic acid in experimentally induced diabetic animals. J Compl Integr Med 2019;17:1–10.10.1515/jcim-2019-0133Search in Google Scholar
46. Rozaini, MZ, Zuki, AB, Noordin, M, Norimah, Y, Hakim, AN. The effects of different types of honey on tensile strength evaluation of burn wound tissue healing. Int J Appl Res Vet Med 2004;2:290–6.Search in Google Scholar
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