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Licensed Unlicensed Requires Authentication Published by De Gruyter July 16, 2021

Antibiofilm action of Persea americana glycolic extract over Acinetobacter baumannii and absence of toxicity in Galleria mellonella

  • Sabrina Ferreira dos Santos Liberato ORCID logo , Mariana Raquel da Cruz Vegian ORCID logo , Amjad Abu Hasna ORCID logo EMAIL logo , Janaína Araújo de Alvarenga , Juliana Guimarães dos Santos , Ítalo Rigotti Pereira Tini , Isabela Amêndola , Juliana Campos Junqueira ORCID logo and Luciane Dias de Oliveira ORCID logo

Abstract

Objectives

This study aimed to evaluate the antibiofilm activity and toxicity of the glycolic extract of Persea americanaP. americana” over multidrug-resistant strains of Acinetobacter baumannii “A. baumannii” as alternative therapy to be investigated.

Methods

A bacterial inoculum of each bacterial strain (4a, 5a, 9a, 12a, ATCC 19606) of A. baumannii was prepared and adjusted by the spectrophotometer. The microdilution broth method was performed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). P. americana glycolic extracts were obtained of the tree stalk and leaves. The biofilm viability was tested by MTT assay after 5 min exposure. The toxicity of the extracts was tested by invertebrate model Galleria mellonella. The data were analyzed by ANOVA, Tukey test and log-rank method (α=0.05).

Results

The extract showed an inhibitory and bactericidal action over all the tested strains with the lowest MIC value observed for the reference strain (3.12 mg/mL). The extract did not demonstrate toxicity in any of the tested concentrations (12.5, 25 and 50 mg/mL) in Galleria mellonella larvae, with a survival percentage above 80% after 168 h.

Conclusions

The glycolic extract of P. americana has microbicidal and antibiofilm activity on multidrug-resistant clinical strains of A. baumannii and showed low toxicity for the invertebrate model G. mellonella.


Corresponding author: Amjad Abu Hasna, Department of Restorative Dentistry, Endodontics division, Institute of Science and Technology, São Paulo State University – UNESP, Av. Eng. Francisco José Longo, 777 – Jardim Sao Dimas, São José dos Campos – SP, 12245-000 São Paulo, Brazil, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: The local Institutional Review Board deemed the study exempt from review.

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Received: 2021-02-03
Accepted: 2021-07-05
Published Online: 2021-07-16

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