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Licensed Unlicensed Requires Authentication Published by De Gruyter April 21, 2022

Evaluation of ethanol extracts from three species of Artocarpus as natural gastroprotective agents: in vivo and histopathological studies

  • Fitrya Fitrya ORCID logo EMAIL logo , Annisa Amriani , Rennie Puspa Novita , Aufa Salsabila , Silvy Marina Siregar and Yunike Anafisya

Abstract

Objectives

Artocarpus is a tropical plant known for its high concentration of secondary metabolites, especially flavonoids and phenolics. This study aims to evaluate the potential use of ethanol extracts obtained from three species of Artocarpus (Artocarpus altilis, Artocarpus champeden, and Artocarpus heterophyllus) as gastroprotective agents.

Methods

The gastroprotective potential of the extracts at concentrations of 100, 200, and 400 mg/kg BW was studied in an animal model induced by absolute ethanol. The animals were subjected to extract pre-treatment for 14 days. Subsequently, the gastroprotective potential was evaluated based on the area of the lesion, the volume of gastric fluid, gastric pH, total acidity, gastric wall mucus content, and microscopic lesions.

Results

There was a significant decrease in the lesion area and gastric fluid volume in the animals that received pre-treated extracts of A. altilis, A. Champeden, and A. heterophyllus, as well as a significant increase in the pH and gastric wall mucus secretion when compared to the ethanol group (p<0.05), rather than the omeprazole or the normal group (p>0.05). Both macroscopic and microscopic examinations revealed a significant decrease in necrotic lesions.

Conclusions

Extracts of A. altilis, A. Champeden, and A. heterophyllus are effective in lowering the risk of peptic ulcers since they have the potential to be used as natural gastroprotective agents.


Corresponding author: Fitrya Fitrya, M.Sc., Department of Pharmacy, Faculty of Mathematic and Natural Science, Universitas Sriwijaya, Jl. Raya Palembang-Prabumulih Km.32, Indralaya 30662, South Sumatera, Indonesia, E-mail:

Funding source: Universitas Sriwijaya

Award Identifier / Grant number: SP DIPA-023.17.2.677515/2021

  1. Research funding: This research was supported by Research and Community Service Institution of Universitas Sriwijaya for research funding through research-grant scheme “Sateks Research Grant Number: SP DIPA-023.17.2.677515/2021.”

  2. Author contribution: Fitrya: Conceptualisation, methodology, Investigation, Data Curation, Writing - Original Draft, Writing - Review & Editing. Annisa Amriani, Rennie Puspa Novita, Aufa Salsabila, Silvy Marina Siregar and Yunike Anafisya: Validation, Formal analysis, Investigation, Data Curation.

  3. Conflict of interest: The authors declare no conflict of interest

  4. Informed consent: Not applicable.

  5. Ethical approval: The procedure for handling test animals was approved by the Health Research Commite in the Health Polytechnic in No. 530/KEPK/Adm2/XII/2020.

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Received: 2022-03-08
Accepted: 2022-03-30
Published Online: 2022-04-21

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