Abstract
Background: The aim of this study is to determine the relationship between oxidative stress marker (8-iso-prostaglandine F2α) and glycemic indices computed from daily glucose monitoring data in children with type 1 diabetes mellitus (T1DM).
Methods: Thirty-one children and adolescents with T1DM (median age 12.2 years) and healthy subjects (median age 11.7 years) were enrolled into the study. Anthropometric data were recorded for the entire group before the study. In addition, diabetes duration, insulin requirement, lipid values, microalbuminuria, HbA1c were recorded in T1DM subjects. T1DM subjects performed self-monitoring of blood glucose (SMBG) for a month (at least four times a day) for calculating glycemic indices. Twenty-four-hour urine 8-iso-prostoglandine F2α levels were studied at the end of the study period in the both groups.
Results: Median diabetes duration was 5 years, hemoglobin A1c (HbA1c) was 7.3%. Standard deviation (SD) of the blood glucose (BG) was determined as 85 mg/dL. Median urinary 8-iso-prostoglandine F2α was found to be significantly higher than that of the healthy subjects (2808.9 and 298 pg/mg creatinine, p<0.001, respectively). There was no correlation between urinary 8-iso-prostoglandine F2α and age, anthropometric data, diabetes duration, insulin requirement, lipid values, microalbuminuria, HbA1c, or SD of BG in T1DM groups.
Conclusions: This study showed that, 8-iso-prostoglandine F2α that is an oxidative stress marker, is significantly higher in T1DM than that of healthy subjects while, no significant relation between glycemic indices and urinary 8-iso-prostoglandine F2α levels were demonstrated. Further studies are needed to assess other factors, and the relationship between glucose fluctuations and oxidative stress markers.
References
1. Craig ME, Hattersley A, Donaghue KC. Definition, epidemiology and classification of diabetes in children and adolescents. Pediatr Diabetes 2009;10(Suppl 12):3–12.10.1111/j.1399-5448.2009.00568.xSearch in Google Scholar
2. Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res 2010;29:1058–70.10.1161/CIRCRESAHA.110.223545Search in Google Scholar
3. Monnier L, Mas E, Ginet C, Michel F, Villon L, et al. Activation of oxidative stress by acute glucose fluctuations compared with sustained chronic hypergycemia in patients with type 2 diabetes. J Am Med Assoc 2006;295:1681–7.10.1001/jama.295.14.1681Search in Google Scholar
4. Quagliaro L, Piconi L, Assaloni R, Martinelli L, Motz E, et al. Intermittent high glucose enhances apoptosis related to oxidative stres in human umbilical vein endothelial cells; the role of protein kinase C and NAD(P)-H-oxidase activation. Diabetes 2003;52:2795–804.10.2337/diabetes.52.11.2795Search in Google Scholar
5. Zaccardi F, Pitocco D, Ghirlanda G. Glycemic risk factors of diabetic vascular complications:the role of glycemic variability. Diabetes Metab Res Rev 2009;25:199–207.10.1002/dmrr.938Search in Google Scholar
6. McCall AL, Cox DJ, Crean J, Gloster M, Kovatchev BP. A novel analytical method for assesing glucose variability:using CGMS in type 1 diabetes Mellitus. Diabetes Technol Ther 2006;8:644–53.10.1089/dia.2006.8.644Search in Google Scholar
7. Moberg E, Kollind M, Lins PE, Adamson U. Estimation of blood glycose variability in patients with insulin dependent diabetes mellitus. Scand J Invest 1993;53:507–14.10.1080/00365519309092547Search in Google Scholar
8. Zachrisson I, Wallensteen M, Dahlquist G. Determinants of blood glycose variability in adolescents with insulin dependent Diabetes Mellitus. Acta Paediatr 1995;84:70–4.10.1111/j.1651-2227.1995.tb13488.xSearch in Google Scholar
9. Mezzetti A, Cipollone F, Cuccurullo F. Oxidative stress and cardiovascular complications in diabetes:isoprostanes as new markers on an old paradigm. Cardiovasc Res 2000;47:475–88.10.1016/S0008-6363(00)00118-8Search in Google Scholar
10. Davì G, Ciabattoni G, Consoli A, Mezzetti A, Falco A, et al. In vivo formation of 8-Iso-Prostaglandin F2α and platelet activation in diabetes mellitus effects of ımproved metabolic control and vitamin E supplementation. Circulation 1999;99:224–9.10.1161/01.CIR.99.2.224Search in Google Scholar
11. Gopaul NK, Änggård EE, Mallet AI, Betteridge DJ, Wolf SP, et al. Plasma 8-epi-PGF2α levels are elevated in individuals with non-insulin dependent diabetes mellitus. Febs Letters 1995;368:225–9.10.1016/0014-5793(95)00649-TSearch in Google Scholar
12. Wentholt IM, Kulik W, Michels RP, Hoekstra JB, DeVries JH. Glucose fluctuations and activation of oxidative stres in patients with type 1 diabetes. Diabetologia 2008;51:183–90.10.1007/s00125-007-0842-6Search in Google Scholar PubMed
13. Meng X, Gong C, Cao B, Peng X, Wu D, et al. Glucose fluctuations in association with oxidative stress among children with T1DM:comparison of different phases. J Clin Endocrinol Metab 2015;100:1828–36.10.1210/jc.2014-2879Search in Google Scholar PubMed
14. Wu D, Gong C, Meng X, Yang Q. Correlation between blood glucose fluctuations and activation of oxidative stress in type 1 diabetic children during the acute metabolic disturbance period. Chin Med J (Engl) 2013;126:4019–22.Search in Google Scholar
15. Răchişan AL, Hruşcă A, Căinap S, Pop TL, Andreica M, et al. The activity of 8-iso-prostaglandin F2alpha as an oxidative stress marker in vivo in paeditric patients with type 1 diabetes mellitus ans associated autoimmunities. Clin Lab 2014;60:253–9.10.7754/Clin.Lab.2013.121141Search in Google Scholar
16. Davi G, Chiarelli F, Santilli F, Pomilio M, Vigneri S, et al. Enhanced lipid peroxidation and platelet activation in the early phase of type 1 diabetes mellitus, role of interleukin-6 and disease duration. Circulation 2003;107:3199–203.10.1161/01.CIR.0000074205.17807.D0Search in Google Scholar PubMed
17. Davi G, Alessandrini P, Mezzetti A, Minotti G, Bucciarelli T, et al. In vivo formation of 8-epi-prostaglandine F2 alpha is increased in hyperchloesterolemia. Aterioscler Thromb Vasc Biol 1997;17:3230–5.10.1161/01.ATV.17.11.3230Search in Google Scholar
18. Kostalanská J, Jakuš V, Barák L. HbA1c and serum levels of advanced glycation and oxidation protein products in poorly and well controlled children and adolescents with type 1 diabetes mellitus. J Pediatr Endocrinol Metab 2009;22:433–42.10.1515/JPEM.2009.22.5.433Search in Google Scholar PubMed
19. Schwedhelm E, Bartling A, Lenzen H, Tsikas D, Maas R, et al. Urinary 8-iso-Prostaglandine F2α as a risk marker in patients with coronary heart disease. A matched case-control study. Circulation 2004;109:843–8.10.1161/01.CIR.0000116761.93647.30Search in Google Scholar PubMed
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