Animal models have shown that the skeletal hormone osteocalcin stimulates testicular testosterone synthesis. To assess whether osteocalcin might be a useful marker to detect pubertal development disorders, we examined osteocalcin plasma concentrations in children and adolescents with and without disorders of pubertal development.
Osteocalcin concentrations were investigated in a total of 244 patients with endocrine disorders (122 males, mean age: 11.87+3.77 years), including patients with precocious puberty and constitutional delay of puberty.
Osteocalcin concentrations were highest among adolescents with precocious puberty and advanced pubertal development (120.60±45.22 ng/mL), while the concentrations were lowest among patients with constitutional delay of puberty (102.20±37.13 ng/mL). Overall, osteocalcin concentrations were strongly correlated with markers of bone metabolism.
Although plasma osteocalcin concentrations are associated with pubertal development in boys, it does not appear to be a useful diagnostic marker for altered pubertal development.
The authors thank Sandy Moore, from Marseille, France, for editorial assistance with the manuscript.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
1. Szulc P, Seeman E, Delmas PD. Biochemical measurements of bone turnover in children and adolescents. Osteoporos Int 2000;11:281–94.Search in Google Scholar
2. Dubnov-Raz G, Ish-Shalom S, Chodick G, Rozen GS, Giladi A, et al. Osteocalcin is independently associated with body mass index in adolescent girls. Pediatr Obes 2012;7:313–8.Search in Google Scholar
3. Motyl KJ, Mccabe LR, Schwartz AV. Bone and glucose metabolism: a two-way street. Arch Biochem Biophys 2010;503:2–10.Search in Google Scholar
4. Lee NK, Sowa H, Hinoi E, Ferron M, Ahn JD, et al. Endocrine regulation of energy metabolism by the skeleton. Cell. 2007;130:456–69.Search in Google Scholar
5. Yoshikawa Y, Kode A, Xu L, Mosialou I, Silva BC, et al. Genetic evidence points to an osteocalcin-independent influence of osteoblasts on energy metabolism. J Bone Miner Res 2011;26:2012–25.Search in Google Scholar
6. Ng K. Regulation of glucose metabolism and the skeleton. Clin Endocrinol (Oxf) 2011;75:147–55.Search in Google Scholar
7. Ducy P. The role of osteocalcin in the endocrine cross-talk between bone remodelling and energy metabolism. Diabetologia 2011;54:1291–7.Search in Google Scholar
8. Karsenty G. Convergence between bone and energy homeostases: Leptin regulation of bone mass. Cell Metab 2006;4:341–8.Search in Google Scholar
9. Gracia-Marco L, Ortega FB, Jimenez-Pavon D, Rodriguez G, Valtuena J, et al. Contribution of bone turnover markers to bone mass in pubertal boys and girls. J Pediatr Endocrinol Metab 2011;24:971–4.Search in Google Scholar
10. Oury F, Sumara G, Sumara O, Ferron M, Chang H, et al. Endocrine regulation of male fertility by the skeleton. Cell 2011;144:796–809.Search in Google Scholar
11. Oury F. A crosstalk between bone and gonads. Ann N Y Acad Sci 2012;1260:1–7.Search in Google Scholar
12. Delemarre-van de Waal H, van Coeverden S, Rotteveel J. Hormonal determinants of pubertal growth. J Pediatr Endocrinol Metab 2001;14(Suppl 6):1521–6.Search in Google Scholar
13. Yilmaz D, Ersoy B, Bilgin E, Gümüşer G, Onur E, et al. Bone mineral density in girls and boys at different pubertal stages: relation with gonadal steroids, bone formation markers, and growth parameters. J Bone Miner Metab 2005;23:476–82.Search in Google Scholar
14. Greulich W, Pyle S. Radiographic atlas of skeletal development of the hand and wrist. 2nd ed. Stanford, CA, USA: Stanford University Press, 1959.Search in Google Scholar
15. Marshall W, Tanner J. Variations in the pattern of pubertal changes in girls. Arch Dis Child 1969;44:291–303.Search in Google Scholar
16. Marshall W, Tanner J. Variations in the pattern of pubertal changes in boys. Arch Dis Child 1970;45:13–23.Search in Google Scholar
17. van Buuren S, Ooms J. Stage line diagram: an age-conditional reference diagram for tracking development. Stat Med 2009;28:1569–79.Search in Google Scholar
18. Schmidt-Gayk H, Spanuth E, Kötting J, Bartl R, Felsenberg D, et al. Performance evaluation of automated assays for beta-Cross Laps, N-MID-Osteocalcin and intact parathyroid hormone (BIOROSE Multicenter Study). Clin Chem Lab Med 2004;42:90–5.Search in Google Scholar
19. Bayer M. Reference values of osteocalcin and procollagen type I N-propeptide plasma levels in a healthy Central European population aged 0-18 years. Osteoporos Int 2014;25:729–36.Search in Google Scholar
20. Saggese G, Baroncelli GI, Bertelloni S. 5 Puberty and bone development. Online 2002;16:53–64.Search in Google Scholar
21. Karsenty G. The mutual dependence between bone and gonads. J Endocrinol 2012;213:107–14.Search in Google Scholar
22. Lehtonen-Veromaa M, Mottonen T, Irjala K, Nuotio I, Leino A, et al. A 1-year prospective study on the relationship between physical activity, markers of bone metabolism, and bone acquisition in peripubertal girls. J Clin Endocrinol Metab 2000;85:3726–32.Search in Google Scholar
23. Gracia-Marco L, Vicente-Rodríguez G, Valtueña J, Rey-López JP, Díaz Martínez AE, et al. Bone mass and bone metabolism markers during adolescence: the HELENA study. Horm Res Paediatr 2010;74:339–50.Search in Google Scholar
24. Flemming GM, Petzold S, Meigen C, Körner A, Kiess W, et al. Is circulating osteocalcin related to adipokines and overweight/obesity in children and adolescents? Exp Clin Endocrinol Diabetes 2012;120:383–7.Search in Google Scholar
25. Kirmani S, Atkinson EJ, Melton LJ, Riggs BL, Amin S, et al. Relationship of testosterone and osteocalcin levels during growth. J Bone Miner Res 2011;26:2212–6.Search in Google Scholar
26. Jürimäe J, Lätt E, Mäestu J, Saar M, Purge P, et al. Osteocalcin is inversely associated with adiposity and leptin in adolescent boys. J Pediatr Endocrinol Metab 2015;28:571–7.Search in Google Scholar
The online version of this article (DOI: 10.1515/jpem-2016-0243) offers supplementary material, available to authorized users.
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