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Licensed Unlicensed Requires Authentication Published by De Gruyter November 11, 2020

Serum level of NPTX1 is independent of serum MKRN3 in central precocious puberty

  • Hwal Rim Jeong , Jong Seo Yoon , Hye Jin Lee , Yeong Suk Shim , Min Jae Kang and Il Tae Hwang EMAIL logo



Makorin ring finger protein 3 (MKRN3) is associated with the initiation of puberty, and loss of function mutation of MKRN3 is the most common genetic cause of central precocious puberty (CPP). A recent study reported that MKRN3 interacts with and suppresses neural pentraxin-1 precursor (NPTX1) activity via polyubiquitination during early puberty in the mouse hypothalamus. This study investigated the correlation between serum NPTX1 and MKRN3 in CPP girls and predicted the potential role of NPTX1 in pubertal progression.


In this case–control study, we examined 34 girls diagnosed with CPP and 34 healthy prepubertal girls. Anthropometric and hormonal parameters were measured and serum levels of NPTX1 and MKRN3 were evaluated with commercial enzyme-linked immunosorbent assay kits.


Serum MKRN3 level decreased significantly in CPP patients compared to controls (344.48 ± 333.77 and 1295.21 ± 780.80 pg/mL, respectively, p<0.001). Serum MKRN3 tended to decrease as Tanner breast stage increased. However, no significant difference was observed in serum NPTX1 levels between patients and controls (20.14 ± 31.75 ng/mL and 12.93 ± 8.28 ng/mL, respectively, p=0.248). The serum level of NPTX1 did not change significantly with the Tanner breast stage. Serum NPTX1 was correlated with the height standard deviation score (r=0.255; p<0.05), but was not correlated with serum MKRN3 level or the others. Conclusion: Although serum NPTX1 level was independent of serum MKRN3 level, the possibility they might be involved in the progression of puberty or CPP remains. Further research is needed to determine their role in the hypothalamus.

Corresponding author: Il Tae Hwang, M.D., Ph.D., Department of Pediatrics, Kangdong Sacred Heart Hospital, Hallym University, College of Medicine, 150 Seonganro, Gangdong-gu, Seoul, Korea, 05338, Phone: +82 2 2224 2251, Fax: +82 2 482 8334, E-mail:

Funding source: Soonchunhyang University Research Fund


This work was supported by the Soonchunhyang University Research Fund

  1. Research funding: None declared.

  2. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  3. Competing interests: The funding organization played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

  4. Ethical approval: This study protocol was approved by the Institutional Review Board of the Hallym Medical Center (KANGDONG 2016-11-003). Consent was obtained from each patient or subject after a full explanation of the purpose and nature of the procedures.


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Received: 2020-07-06
Accepted: 2020-08-31
Published Online: 2020-11-11
Published in Print: 2021-01-27

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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