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Licensed Unlicensed Requires Authentication Published by De Gruyter February 21, 2020

Vertebrate prey composition analysis of the Pallas’s cat (Otocolobus manul) in the Gongga Mountain Nature Reserve, based on fecal DNA

  • Dong Zhao , Chuangming Yang , Jinnan Ma , Xiuyue Zhang and Jianghong Ran ORCID logo EMAIL logo
From the journal Mammalia


Diet analysis is essential to fully understanding the biology of a species and its function within the ecosystem, and is also key in identifying food web interactions and the population dynamics of predators and prey. There is currently little information on the diet of small to mid-sized carnivores due to their elusive behavior. We used a DNA-based method to analyze the vertebrate prey of the Pallas’s cat (Otocolobus manul) in the Gongga Mountain Nature Reserve of southwestern China, a global biodiversity hotspot region. We confirmed 14 scat samples containing the Pallas’s cat DNA from 100 small carnivore scats collected at altitudes between 3200 and 4900 m, and then performed polymerase chain reaction (PCR) amplifications using primers targeting the vertebrate mitochondrial 12S rRNA gene with a blocking oligonucleotide specific to the predator sequence. We identified a total of 18 prey taxa, including nine mammals, eight birds and one fish. Our results show that pikas (in 79% of the scats) and birds (in 50% of the scats) are the predominant prey consumed by the Pallas’s cat in our investigation area. The most important finding in our study is that Cypriniformes species were found in the Pallas’s cat’s diet, exhibiting dietary diversification consistent with the accessible fauna of the surrounding environment. In order to increase the detectability of both typical and rare vertebrate prey items, we constructed a blocking primer to inhibit the amplification of the Pallas’s cat DNA.


We are grateful to Xiaohong Sun, Jie Gao, Gai Luo, Xingcheng He and Meiling Cheng for their kind help and suggestions in conducting the research and data analyses. We also thank Professor Yongjie Wu, Mike Seitz and two additional anonymous reviewers for their valuable comments on the manuscript. This study was funded by the Sichuan Gongga Mountain National Nature Reserve central finance forestry protection 2014 subsidy fund snow leopard investigation project.

  1. Competing interests: The authors declare that they have no competing interests.

  2. Availability: The datasets used in the present study are available from the corresponding author on reasonable request.

  3. Consent for publication: Applicable.

  4. Ethics approval and consent to participate: The experiments comply with the current laws of China in which they were performed.


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Supplementary Material

The online version of this article offers supplementary material ( 0144).

Received: 2019-08-18
Accepted: 2019-12-17
Published Online: 2020-02-21
Published in Print: 2020-09-25

©2020 Walter de Gruyter GmbH, Berlin/Boston

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