Crystal structure of (5 R ,8 R ,9 R ,10 R ,12 R , 13 R ,14 R ,17 S )-2-( E -3- ﬂ uorobenzylidene)- 12-hydroxy-4,4,8,10,14-pentamethyl-17-(( R )- 2,6,6-trimethyltetrahydro-2 H -pyran-2-yl) hexadecahydro-3 H -cyclopenta[ a ]phenanthren-3-one, C 37 H 53 FO 3

C

The residue was extracted with ethyl acetate, washed with saturated brine, dried with anhydrous sodium sulfate, filtered, concentrated under reduced pressure to obtain the crude product, and the crude product was recrystallized with ethyl acetate to obtain the pure product of 20(R)-panaxadiol.20(R)-panaxadiol and PCC (pyridinium chlorochromate) were dissolved in 25 mL of dichloromethane, and the reaction mixture was stirred at room temperature for 4 h.The intermediate 20(R)-3-oxopanaxadiol was purified by silica gel column chromatography.Methanol (1.4 mL), 20(R)-3-oxopanaxadiol (100 mg, 0.22 mmol), and 2-fluorobenzaldehyde (0.023 mL, 0.22 mmol) were added to a round-bottomed flask, to which 0.72 mL (25 %) aqueous sodium hydroxide was added dropwise, and the flask was stirred for 4 h at room temperature.The reaction was monitored by thin layer chromatography (TLC, 254 nm).At the end of the reaction, appropriate amount of water was added, the reaction mixture was transferred to a partition funnel and extracted with ethyl acetate (twice), the organic phases were combined and washed with saturated sodium chloride solution, dried with anhydrous sodium sulfate, filtered, concentrated under reduced pressure and the crude product was purified by silica gel column chromatography.Recrystallization with ethyl acetate solution gave crystals of the target compound.

Experimental details
The H atoms were placed in idealized positions and treated as riding on their parent atoms [3].

Comment
Ginseng saponins include protopanoxadiol-type saponin, panaxatriol-type saponin and oleanane-type saponin, sharing a tetrahydrofuran ring and a dammarane skeleton [4].In recent years, our laboratory has carried out research on the synthesis and single crystal X-ray diffraction of protopanoxadiol derivatives [5][6][7] and protopanaxatriol derivatives [8,9], and the title compound is a 20(R)-panaxadiol derivative.Ginsenosides are important saponins among ginsenosides and their structures can be modified [10].
The ORTEP diagram is presented in the Figure .The title compound contains one drug molecule in the asymmetric unit (cf. the figure).Bond lengths and angles are all in the expected ranges.The title compound has a dammarane type as the parent structure [11], with a benzene ring structure attached to C(1).An α,β-unsaturated ketone group is formed between the benzene ring and the parent nucleus through There is a fluorine substitution on the benzene ring.The benzene ring is non-coplanar with the saturated cyclic ketone and the dihedral angle between them is 54.048°.This twisted configuration may increase the likelihood of interactions with bioactive molecules or the purposes of creating more potent biological activity [12,13] 18) is 1.374 Å.It is assumed that the dihedral angles between the fivemembered ring and the pyran ring and the six-membered ring where the hydroxyl group is located are small, 3.031 Å and 0.604 Å, respectively, probably due to hydrogen bonding.The compound has a total of 8 chiral carbons, which are: 5R,8R,9R, 10R,12R,13R,14R,17S.
. The bond length of C(18)-O(1) is 1.4564 Å.The bond length of C(22)-O(1) is 1.4585 Å.There is a pyran ring substitution at the C(17) position and the oxygen atom on the ring acts as a hydrogen bond acceptor to form an intramolecular hydrogen bond.The bond length of C(13)-O(2) is 1.4251 Å.The pyran ring is connected to the five-membered ring via C(17)-C(18), the bond length of C(17)-C(

Table  :
Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å  ).

Table  :
Data collection and handling.