Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter October 11, 2019

Application of calcium carbonate nanocarriers for controlled release of phytodrugs against Xylella fastidiosa pathogen

Francesca Baldassarre, Angelo De Stradis, Giuseppe Altamura, Viviana Vergaro, Cinzia Citti, Giuseppe Cannazza, Agostina L. Capodilupo, Luciana Dini and Giuseppe Ciccarella

Abstract

Calcium carbonate-based hollow or porous particles are one of the preferred carriers for fabrication of drug delivery systems. We have developed an eco-friendly method to produce calcium carbonate nanocrystals, which have shown biocompatibility and optimal capacity to across cell membrane in human cell lines providing new tools in cancer therapy. The success of drug delivery systems has paved the way for the development of systems for controlled release of agrochemicals. In this work, we exploited calcium carbonate nanocrystals as carriers for targeted release of phytodrugs investigating a potential control strategy for the pathogen Xylella fastidiosa. This pathogen is the causal agent of the Olive Quick Decline Syndrome that is an unprecedented emergency in Italy and potentially in the rest of Europe. We studied nanocrystals interactions with bacteria cells and the application in planta to verify olive plants uptake. Ultrastructural analysis by electron microscopy shown an alteration of bacteria wall following nanocrystals interaction. Nanocrystals were adsorbed from roots and they translocated in plants tissues. Calcium carbonate carriers were able to encapsulate efficiently two types of antimicrobial substances and the potential efficacy was tested in experiment under greenhouse conditions.

Acknowledgments

We thank Prof. Giorgio Mariano Balestra for critical reading and helpful reviewing of the manuscript. We also thank Dr. Maria Saponari for providing the bacterial strain of Xylella fastidiosa and review the discussion of results. Project “Tecnologie Abilitanti per Produzioni Agroalimentari Sicure e Sostenibili-T.A.P.A.S.S”. Cluster Tecnologici Regionali 2014-PELM994 (CUP B38C14002040008). Project “Trattamenti Fitoterapici innovAtivi a base di veTtori di chitosAno-FATA”, Bando pubblico di ricerca per la presentazione di proposte progettuali Cod. A, n. 1410 del 12 giugno 2015 “Approvazione delle linee guida per il parco della ricerca e sperimentazione finalizzata alla prevenzione e al contenimento del complesso del disseccamento rapido dell’olivo (CODIRO)” CUP B36J16002170007. Project “Olivicoltura e difesa da Xylella fastidiosa e da insetti vettori in Italia – Oli.Di.X.I.It”, prot. Mipaaf n.0011485 del 05/04/2017 (Ministero delle politiche agricole alimentari e forestali).

References

[1] Y. Boyjoo, V. K. Pareek, J. Liu. J. Mater. Chem. A2, 14270 (2014).Search in Google Scholar

[2] N. G. M. Palmqvist, J. M. Nedelec, G. A Seisenbaev, V. G. Kessler. Acta Biomater. 57, 426 (2017).Search in Google Scholar

[3] F. Baldassarre, V. Vergaro, F. Scarlino, F. De Santis, G. Lucarelli, A. della Torre, G. Ciccarella, R. Rinaldi, G. Giannelli, S. Leporatti. Macromol. Biosci.12, 656 (2012).Search in Google Scholar

[4] J. B. Peters, H. Bouwmeester, S. Gottardo, V. Amenta, M. Arena, P. Brandhoff, H. J. P. Marvin, A. Mech, F. B. Moniz, L. Q. Pesudo, H. Rauscher, R. Schoonjans, A. K. Undas, M. V. Vettori, S. Weigel, K. Aschberger. Trends Food Sci. Technol.54, 155 (2016).Search in Google Scholar

[5] E. Fortunati, A. Mazzaglia, G. M. Balestra, J. Sci. Food Agric.99, 986 (2019).Search in Google Scholar

[6] Md. Nuruzzaman, M. M. Rahman, Y. Liu, R. Naidu. J. Agric. Food Chem. 64, 1447 (2016).Search in Google Scholar

[7] D. Tilman, K. G. Cassman, P. A. Matson, R. Naylor, S. Polasky. Nature7, 418 (2002).Search in Google Scholar

[8] M. M. Rui, C. X. Ma, Y. Hao, J. Guo, Y. K. Rui, X. L. Tang, Q. Zhao, X. Fan, Z. T. Zhang, T. Q. Hou, S. Y. Zhu. Front. Plant Sci.7, 815 (2016).Search in Google Scholar

[9] H. Chen, R. Yada. Trends Food Sci. Technol.22, 585 (2011).Search in Google Scholar

[10] J. A. Tapia-Hernández, P. I. Torres-Chávez, B. Ramírez-Wong, A. Rascón-Chu, M. Plascencia-Jatomea, C. G. Barreras-Urbina, N. A. Rangel-Vázquez, F. Rodríguez-Félix. J. Agric. Food Chem. 63, 4699 (2015).Search in Google Scholar

[11] S. Kumar, N. Chauhan, M. Gopal, R. Kumar, N. Dilbaghi. Int. J. Biol. Macromol.81, 631 (2015).Search in Google Scholar

[12] A. Perez-de-Luque, D. Rubiales. Pest Manag. Sci.65, 540 (2009).Search in Google Scholar

[13] L. R. Khot, S. Sankaran, J. M. Maja, R. Ehsani, E. W. Schuster. Crop Prot.35, 64 (2012).Search in Google Scholar

[14] E. E. Hafez, H. S. Hassan, M. F. Elkady, E. Salama. Int. J. Sci. Technol. Res.3, 318 (2014).Search in Google Scholar

[15] Y. Hao, X. Cao, C. Ma, Z. Zhang, N. Zhao, A. Ali, T. Hou, Z. Xiang, J. Zhuang, S. Wu, B. Xing, Z. Zhang, Y. R. Front. Plant Sci. 8, 1332 (2017).Search in Google Scholar

[16] Z. Zabrieski, E. Morrell, J. Hortin, C. Dimkpa, J. McLean, D. Britt, A. Anderson. Ecotoxicology24, 1305 (2015).Search in Google Scholar

[17] A. Panáček, M. Kolář, R. Večeřov, R. Prucek, J. Soukupov, V. Kryštof, P. Hamal, R. Zbořil, L. Kvítek. Biomaterials30, 6333 (2009).Search in Google Scholar

[18] S. W. Kim, J. H. Jung, K. Lamsal, Y. S. Kim, J. S. Min, Y. S. Lee. Mycobiology40, 53 (2012).Search in Google Scholar

[19] C. Marambio-Jones, E. M. V. Hoek. J. Nanoparticle Res.12, 1531 (2010).Search in Google Scholar

[20] G. A. Martinez-Castanon, N. Nino-Martınez, F. Martınez-Gutierrez, J. R. Martınez-Mendoza, F. Ruiz. J. Nanoparticle Res.10, 1343 (2008).Search in Google Scholar

[21] H.-J. Park, S. H. Kim, H. J. Kim, S.-H. Choi. Plant Pathol. J.22, 295 (2006).Search in Google Scholar

[22] J. S. Min, K. S. Kim, S. W. Kim, J. H. Jung, K. Lamsal, S. B. Kim, M. Y. Jung, Y. S. Lee. Plant Pathol. J.25 376 (2009).Search in Google Scholar

[23] J. Thiel, L. Pakstis, S. Buzby, M. Raffi, C. Ni, D. J. Pochan, S. I. Shah. Small3, 799 (2007).Search in Google Scholar

[24] E. Battiston, M. C. Salvatici, A. Lavacchi, A. Gatti, S. Di Marco, L. Mugnaia. Pest Manag. Sci. 74, 1903 (2018).Search in Google Scholar

[25] M. Shenashen, A. Derbalah, A. Hamza, A. Mohamedd, S. El Safty. Pest Manag. Sci.73, 1121 (2016).Search in Google Scholar

[26] Z. Piperigkou, K. Karamanou, A. B. Engin, C. Gialeli, A. O. Docea, D. H. Vynios, M. S. G. Paväo, K. S. Golokhvast, M. Shtilman, A. Argiris, E. Shishatskaya, A. M. Tsatsakis. Food Chemi. Toxicol.91, 57 (2016).Search in Google Scholar

[27] K. J. Dietz, S. Herth. Trends Plant Sci.16, 582 (2011).Search in Google Scholar

[28] P. Wang, E. Lombi, F. J. Zhao, P. M. Kopittke. Trends Plant Sci.21, 699 (2016).Search in Google Scholar

[29] C. M. Rico, S. Majumdar, M. Duarte-Gardea, J. R. Peralta-Videa, J. L. Gardea-Torresdey. J. Agric. Food Chem. 59, 3485 (2011).Search in Google Scholar

[30] T. Souhoa, L. Lambonia, L. Xiao, G. Yang. Biotechnol. Adv.36, 1928 (2018).Search in Google Scholar

[31] V. Vergaro, P. Papadia, S. Leporatti, S. A. De Pascali, F. P. Fanizzi, G. Ciccarella. J. Inorg. Biochem. 153, 284 (2015).Search in Google Scholar

[32] F. Baldassarre, C. Allegretti, D. Tessaro, E. Carata, C. Citti, V. Vergaro, C. Nobile, G. Cannazza, P. D’Arrigo, A. Mele, L. Dini, G. Ciccarella. ChemistrySelect1, 6507 (2016).Search in Google Scholar

[33] V. Vergaro, E. Carata, F. Baldassarre, E. Panzarini, L. Dini, C. Carlucci, S. Leporatti, B. F. Scremin, D. Altamura, C. Giannini, G. Ciccarella. Adv. Powder Technol.28, 2445 (2017).Search in Google Scholar

[34] V. Vergaro, M. Civallero, C. Citti, M. Cosenza, F. Baldassarre, G. Cannazza, S. Pozzi, S. Sacchi, F. P. Fanizzi, G. Ciccarella. Cancers10, 31 (2018).Search in Google Scholar

[35] G. Loconsole, O. Potere, D. Boscia, G. Altamura, K. Djelouah, T. Elbeaino, D. Frasheri, D. Lorusso, F. Palmisano, P. Pollastro, M. R. Silletti, N. Trisciuzzi, F. Valentini, V. Savino, M. Saponari. J. Plant Pathol.96, 7 (2014).Search in Google Scholar

[36] P. J. Zarco-Tejada, C. Camino, P. S. A. Beck, R. Calderon, A. Hornero, R. Hernández-Clemente, T. Kattenborn, M. Montes-Borrego, L. Susca, M. Morelli, V. Gonzalez-Dugo, P. R. J. North, B. B. Landa, D. Boscia, M. Saponari, J. A. Navas-Cortes. Nat. Plants4, 432 (2018).Search in Google Scholar

[37] M. Saponari, D. Boscia, G. P. Martelli. Acta Hortic.1199, 251 (2018).Search in Google Scholar

[38] D. L. Hopkins. Annu. Rev. Phytopathol.27, 271 (1989).Search in Google Scholar

[39] F. Navarrete, L. De La Fuente. Appl. Environ. Microbiol.80, 1097 (2014).Search in Google Scholar

[40] P. Wang, Y. Lee, M. M. Igo, M, C. Roper. Mol. Plant Pathol.18, 7 (2016).Search in Google Scholar

[41] C. E. Maddox, L. M. Laur, L. Tian. Curr. Microbiol.60, 53 (2010).Search in Google Scholar

[42] L. S. Muranaka, T. E. Giorgiano, M. A. Takita, M. R. Forim, L. F. C. Silva, H. D. Coletta-Filho, M. A. Machado, A. A. de Souza. PLoS One8, 72937 (2013).Search in Google Scholar

[43] G. P. Martelli, M. Russo. Methods Virol.8, 143 (1984).Search in Google Scholar

[44] A. L. Capodilupo, V. Vergaro, E. Fabiano, M. De Giorgi, F. Baldassarre, A. Cardone, A. Maggiore, V. Maiorano, D. Sanvitto, G. Giglibe, G. Ciccarella. J. Mater. Chem. B3, 3315 (2015).Search in Google Scholar

[45] J. Hernandez-Montelongo, V. F. Nascimento, D. Murillo, T. B. P. Taketa, Sahoo, A. A. de Souza, M. M. Beppu, M. A. Cotta. Carbohyd. Polym.136, 1 (2016).Search in Google Scholar

[46] T. J. Silhavy, D. Kahne, S. Walker. Cold Spring Harb. Perspect. Biol.2, a000414 (2010).Search in Google Scholar

[47] H. H. Mollenhauer, D. L. Hopkins. J. Bacteriol.119, 612 (1974).Search in Google Scholar

[48] J. N. Chen, S. L. Li, J. X. Luo, R. S. Wang, W. Ding. J. Nanomat.1, 1 (2016).Search in Google Scholar

[49] C. Schwechheimer, C. J. Sullivan, M. J. Kuehn. Biochemistry52, 3031 (2013).Search in Google Scholar

[50] M. Ionescu, P. A. Zaini, C. Baccari, S. Tran, A. M. da Silva, S. E. Lindow. Proc. Natl. Acad. Sci. U S A111, E3910 (2014).Search in Google Scholar

[51] M. Scortichini, J. Chen, M. de caroli, G. Dalessandro, N. Pucci, V. Modesti, A. L’aurora, M. Petriccione, L. Zampella, F. Mastrobuoni, D. Migoni, L. Del Coco, C. R. Girelli, F. Piacente, N. Cristella, P. Marangi, F. Laddomada, M. Di Cesare, G. Cesari, F. P. Fanizzi, S. Loreti. Phytopathol. Mediterr.57, 48 (2018).Search in Google Scholar

[52] V. Vergaro, E. Carata, E. Panzarini, F. Baldassare, L. Dini, G. Ciccarella. AIP Conf. Proc.1667, 020014 (2015).Search in Google Scholar

[53] L. F. Cruz, P. A. Cobine, L. De La Fuente. Appl. Environ. Microbiol. 78, 1321 (2012).Search in Google Scholar

[54] X. Pan, S. Chen, D. Li, W. Rao, Y. Zheng, Z. Yang, L. Li, X. Guan, Z. Chen. Front. Chem. 5, 130 (2018).Search in Google Scholar

[55] E. Fortunati, G. M. Balestra. J. Nanomed. Nanotechnol.2, 115 (2018).Search in Google Scholar

[56] A. Rossetti, A. Mazzaglia, M. Muganu, M. Paolocci, M. Sguizzato, E. Esposito, R. Cortesi, G. M. Balestra. J. Plant Dis. Protect.124, 563, (2017).Search in Google Scholar

Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/pac-2018-1223).

Published Online: 2019-10-11
Published in Print: 2020-03-26

©2020 IUPAC & De Gruyter. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. For more information, please visit: http://creativecommons.org/licenses/by-nc-nd/4.0/