Accessible Requires Authentication Published by De Gruyter October 11, 2014

Influence of the physicochemical environment on membrane-protein binding: investigation using a fabricated PVDF membrane

Abdul L. Ahmad, Norhidayah Ideris, Ooi Boon Seng, Low Siew Chun and Asma Ismail

Abstract

The correlation between the behavior of a polyvinylidene fluoride (PVDF) membrane toward protein binding at different physicochemical environments was investigated. The PVDF membrane was first fabricated and characterized to understand its morphological, polymorph and intrinsic characteristics. The results confirmed that the membrane had a microporous, symmetric structure and high hydrophobicity and electronegativity. The membrane was further tested under different physicochemical environments by modifying the type of protein and pH medium used. The results showed that different proteins and pH values contribute to different membrane-protein binding mechanisms. Overall, the results of PVDF membrane-protein binding were satisfactory and demonstrated the ability of the membrane to capture various proteins or reagents.


Corresponding author: Abdul L. Ahmad, School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, Seri Ampangan, 14300 Nibong Tebal, S.P.S, Penang, Malaysia, e-mail:

Acknowledgments

The authors acknowledge Universiti Sains Malaysia, USM (Vice-Chancellor Award 2010, USM-PGRS Grant (8034059), USM Membrane Cluster (8610012) and ERGS Grant (6730004) for financial support.

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Received: 2013-9-8
Accepted: 2014-9-10
Published Online: 2014-10-11
Published in Print: 2015-5-1

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