Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter June 11, 2020

Evaluation of the bioactive properties of Alternanthera sessilis extract and development of sodium alginate – Alternanthera sessilis membrane for wound management

  • Ramaswamy Rajendran , Mariappan Abirami , Elango Santhini , Selvaraj Dinesh Kirupha and Ketankumar Vadodaria ORCID logo EMAIL logo


Many herbal/botanical medicines from plant sources are being used for therapeutic purpose. Alternanthera sessilis sp. plant leaves are known for their phytochemical constituents, which have potent antibacterial activity. In the present study, the properties of the A. sessilis sp. extract and the biocomposite membrane of A. sessilis sp. with sodium alginate were studied for their biomedical applications. Results showed that the A. sessilis ethanoic extract possessed high levels of antioxidant and antimicrobial activity and no cytotoxic effect under in vitro conditions. Similarly, the biocomposite membrane of alginate with the A. sessilis sp. ethanolic extract showed high antibacterial activity with hemocompatibility and swelling characteristics. Although the antioxidant, antimicrobial and cytotoxic effects of A. sessilis have been previously reported, this is the first report on the preparation of a biocomposite membrane with alginate using A. sessilis for biomedical applications.


The authors are thankful to Dr. Prakash Vasudevan, Director of SITRA, for allowing us to carry out the study and for providing the necessary infrastructure for the successful completion of the present research work.


[1] Koehn FE, Carter GT. Nat. Rev. Drug Discov. 2005, 4, 206–220.10.1038/nrd1657Search in Google Scholar PubMed

[2] Leeds JA, Schmitt EK, Krastel P. Expert Opin. Investig. Drugs 2006, 15, 211–226.10.1517/13543784.15.3.211Search in Google Scholar PubMed

[3] Maver T, Maver U, Kleinschek KS, Smrke DM, Kreft S. Int. J. Dermatol. 2015, 54, 740–751.10.1111/ijd.12766Search in Google Scholar PubMed

[4] Napavichayanun S, Aramwit P. J. Biomater. Sci. Polym. Ed. 2017, 28, 703–729.10.1080/09205063.2017.1301772Search in Google Scholar PubMed

[5] Barron C, He JQ. J. Biomater. Sci. Polym. Ed. 2017, 28, 1245–1255.10.1080/09205063.2017.1318030Search in Google Scholar PubMed

[6] Jin F, Xiang Q, Chen X, Peng X, Xing X. J. Biomater. Sci. Polym. Ed. 2016, 27, 1447–1461.10.1080/09205063.2016.1210861Search in Google Scholar PubMed

[7] Mishra KP, Ganju L, Sairam M, Banerjee PK, Sawhney RC. Biomed. Pharmacother. 2008, 62, 94–98.10.1016/j.biopha.2007.06.012Search in Google Scholar PubMed

[8] Hundiwale JC, Patil AV, Kulkarni MV, Patil DA, Mali RG. J. Pharm. Res. 2012, 5, 1924–1929.Search in Google Scholar

[9] Anandkumar BH, Sachidanand YN. Indian J. Dermatol. 2001, 46, 138–141.Search in Google Scholar

[10] Gupta AK. Indian Medicinal Plants, ICMR, New Delhi, 2004.Search in Google Scholar

[11] Aderibigbe BA, Buyana B. Pharmaceutics 2018, 10, 42.10.3390/pharmaceutics10020042Search in Google Scholar PubMed PubMed Central

[12] Augst AD, Kong HJ, Mooney DJ. Macromol. Biosci. 2006, 6, 623–633.10.1002/mabi.200600069Search in Google Scholar PubMed

[13] Qin Y. Int. Wound J. 2005, 2, 172–176.10.1111/j.1742-4801.2005.00101.xSearch in Google Scholar PubMed PubMed Central

[14] Baranoski S, Ayello EA. Wound Care Essentials: Practice Principles, Lippincott Williams & Wilkins: Philadelphia, PA, USA, 2008, 1–576.Search in Google Scholar

[15] Liakos I, Rizzello L, Scurr DJ, Pompa PP, Bayer IS, Athanassiou A. Int. J. Pharm. 2014, 463, 137–145.10.1016/j.ijpharm.2013.10.046Search in Google Scholar PubMed

[16] Valgas C, Souza SM, Smania EF, Smania JA. Braz. J. Microbiol. 2007, 38, 369–380.10.1590/S1517-83822007000200034Search in Google Scholar

[17] ASTM E2149-13a. Standard Test Method for Determining the Antimicrobial Activity of Antimicrobial Agents under Dynamic Contact Conditions, ASTM International, West Conshohocken, PA, 2013.Search in Google Scholar

[18] Dalton T, Dowd SE, Wolcott RD, Sun Y, Watters C, Griswold JA, Rumbaugh KP. PLoS One 2011, 6, e27317.10.1371/journal.pone.0027317Search in Google Scholar PubMed PubMed Central

[19] Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TCD, Coube CS, Leitão SG. Phytother. Res. 2001, 15, 127–130.10.1002/ptr.687Search in Google Scholar PubMed

[20] Prieto P, Pineda M, Anguilar M. Anal. Biochem. 1999, 269, 337–341.10.1006/abio.1999.4019Search in Google Scholar PubMed

[21] Bhattacharyya S, Datta S, Mallick B, Dhar P, Ghosh S. J. Agric. Food Chem. 2010, 58, 8259–8264.10.1021/jf101262eSearch in Google Scholar PubMed

[22] Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Springer (India) Pvt. Ltd, New Delhi, 1998.Search in Google Scholar

[23] Pereira R, Carvalho A, Vaz DC, Gil MH, Mendes A, Bártolo P. Int. J. Biol. Macromol. 2013, 52, 221–230.10.1016/j.ijbiomac.2012.09.031Search in Google Scholar PubMed

[24] Chhatri A, Bajpai J, Bajpai AK. Biomatter 2011, 1, 189–197.10.4161/biom.19005Search in Google Scholar PubMed PubMed Central

[25] Xu Z, Hsia HC. Ann. Plas. Surg. 2018, 1, 113–123.10.1097/SAP.0000000000001450Search in Google Scholar PubMed

[26] Genilloud O. Antonie van Leeuwenhoek 2014, 106, 173–188.10.1007/s10482-014-0204-6Search in Google Scholar PubMed

[27] Kota S, Govada VR, Anantha RK, Verma MK. Agric. Biotechnol. 2017, 10, 197–203.10.1016/j.bcab.2017.03.008Search in Google Scholar

[28] Borah A, Yadav R, Unni B. Int. J. Pharm. Sci. Res. 2011, 2, 1502–1506.Search in Google Scholar

[29] Jalalpure SS, Agrawal N, Patil M, Chimkode R, Tripathi A. Int. J. Green Pharm. 2008, 2, 141–144.10.4103/0973-8258.42729Search in Google Scholar

[30] Raman N. Phytochemical Techniques, New India Publishing Agency: New Delhi, India, 2008, 56–57.Search in Google Scholar

[31] Tsuchiya H, Sato M, Miyazaki T, Fujiwara S, Tanigaki S, Ohyama M, Tanaka T, Iinuma M. J. Ethnopharmacol. 1996, 50, 27–34.10.1016/0378-8741(96)85514-0Search in Google Scholar

[32] Fitzmaurice SD, Sivamani RK, Isseroff RR. Skin Pharmacol. Physiol. 2011, 24, 113–126.10.1159/000322643Search in Google Scholar PubMed

[33] Ashokkumar R, Ramaswamy M. Int. J. Curr. Microbiol. Appl. Sci. 2014, 3, 395–396.Search in Google Scholar

[34] Balaji A, Jaganathan SK, Ismail AF, Rajasekar R. Int. J. Nanomed. 2016, 11, 4339–4355.10.2147/IJN.S112265Search in Google Scholar PubMed PubMed Central

Received: 2019-08-08
Accepted: 2019-12-03
Published Online: 2020-06-11
Published in Print: 2020-07-28

©2020 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 26.9.2023 from
Scroll to top button