Antimicrobial preservation ef ﬁ cacy of liquid glucose and liquid maltitol syrups with and without 0.1% sorbic acid

Objectives: Amongst paediatric pharmaceutical forms, syrups offer advantages such as ease of administration and good palatability. They also exhibited microbial self-preservation properties that may be useful to enhance shelf life of liquid formulation. The objective of our works is to test the self-preservation efficacy of maltitol and glucose syrup without or with sorbic acid as described in the European pharmacopoeia. Methods: The European Pharmacopoeia test of antimicrobial preservation efficacy was performed on liquid glucose syrup and liquid maltitol syrup with and without 0.1% sorbic acid. Results: Unpreserved glucose and maltitol syrups did not meet the European Pharmacopoeia acceptance criteria for antimicrobial preservative efficacy due to the regrowth of Aspergillus brasiliensis on day 28 whereas glucose and maltitol syrups with 0.1% sorbic acid pass the test. Conclusions: The addition of a preservative (sorbic acid) in glucose and maltitol syrups allows the validation of the antimicrobial preservative efficacy test of the European Pharmacopoeia. Further tests are needed to see if preservative efficacy is maintained despite dilutions or in the presence of active pharmaceutical ingredients.


Introduction
Among the paediatric oral forms, liquid forms offers advantages such as their ease of dosage and administration and their acceptability [1] however, the presence of water can reduce the physico-chemical and microbiological stability compared to dry forms (e.g. powder, tablets).
Preservatives can be used to improve the antimicrobial stability of liquid forms although care must be taken not to introduce excipients that are toxic to children. For instance, parabens (e.g. methylparabens, ethylparabens, propylparabens) are well-known preservatives that may be associated with hyperbilirubinemia, hypersensitivity reactions and oestrogenic effects in children, which justify daily dose limits [2]; benzoic acid may also be associated with kernicterus and hypersensitivity reactions in children [3]. Among preservatives, sorbic acid may be preferred for paediatric formulation due to its low toxicity [4]. It is generally recognised as safe, producing only irritant or allergic reactions when applied to the skin. And sorbic acid is used in foods and pharmaceuticals at concentrations ranging from 0.05 to 0.2% and its acceptable daily intake has been set at up to 25 mg/kg of bodyweight in adults [4].
It's also possible to improve the overall microbial stability of formulations by adjusting its pH, water activity, and the nutrients contents of formulations [5]. For instance high osmolarity (low water activity) of syrups allows for selfpreservation and thus limits the addition of preservatives [6]. Indeed, syrups are liquid forms containing a high concentration of soluble carbohydrates. And, by increasing the viscosity of the medium and with their sweetening properties they may be useful to hide the bitterness of an active pharmaceutical ingredient (API) and therefore increase patient compliance [7]. Traditionally, the simple syrup based on sucrose is used, but low caloric and/or non-cariogenic polyols syrups (e.g. sorbitol syrups, maltitol syrups, glucose syrups) may be preferred for paediatric formulations [8,9].
As no data were found in the literature regarding selfpreservation efficacy of liquid maltitol syrups (composed of maltitol, sorbitol and hydrogenated oligo-and polysaccharides) and liquid glucose syrups (composed of glucose and maltodextrins) regarding a pharmaceutical use we would like to study it with and without the addition of sorbic acid to see if they can be a good starting point for the formulation of paediatric drugs.

Materials
Following syrups were studied, the maltitol syrup (Lycasin ® 80/55 Maltitol Syrup, Roquette, France) and the glucose syrup (Glucose Syrup 4779, Roquette, France). Sorbic acid (Inresa, France) has been added in these syrups at its usual concentration of 0.1% (m/v) in the final product. The

Efficacy of antimicrobial preservation
The European Pharmacopoeia test of antimicrobial preservation efficacy was performed [10].
The Colony visual aspect, and C. albicans, P. aeruginosa, S. aureus odour were checked by experienced technician and pharmacists.
Afterwards, 10 8 CFU/mL suspensions of these different microorganisms were prepared in 0.9% sodium chloride using the MacFarland method [11]. A. brasiliensis suspension was obtained using 0.5 g/L of polysorbate 80 to avoid spore sedimentation as recommended by the European pharmacopoeia. Concentrations of these initial inoculum were controlled by plate sedimentation. Briefly, 1/10th serial dilutions of these suspensions were performed to produce 10 3 , 10 2 and 10 1 CFU/ mL suspensions and 100 µL of these suspensions were plated on appropriate agar medium and incubated at the appropriate temperature depending on the germ as described above.
Syrups with and without sorbic acid were placed in 50 mL borosilicate glass bottles closed with GL32 caps. They were then inoculated with 200 µL of these 10 8 CFU/mL suspensions to obtain a final concentration of 10 6 CFU/mL in syrups. The dilutions produced by inoculation were considered negligible as volumes of inoculum did not exceed 1% of the volume of inoculated syrups (final volume 20 mL). The eight inoculated syrups produced were incubated at 20°C (protected from light) for 28 days. On days 0, 14 and 28, 1/10th serial dilution were performed with 1 mL of each syrup to count viable germs as described for the control of initial inoculum. Syrups were gently shaken after inoculation and before each manipulation and plates with 30-300 UFC were selected to count viable germs.
All plating were duplicated. Syrup initial contamination or unwanted contamination of inoculated syrups was checked visually when counting germs on plates.

Results
All inoculum's concentrations were in accordance with expected results (∼10 8 CFU/mL) as well as all count at day 0 (∼10 6 CFU/mL). Both unpreserved syrups (glucose and maltitol) meet pharmacopoeia acceptance criteria on day 14 with a log reduction greater than three for bacteria and greater than one for fungi on day 14. However, they both failed to meet acceptance criteria on day 28 with a growth of A. brasiliensis being observed when compared to day 14.
When adding 0.1% sorbic acid, both preserved syrups finally meet the European Pharmacopoeia acceptance criteria on days 14 and 28 (Table 1).
No external or initial contamination was observed during this study with colonies having the same visual aspect and odour than initial strains.

Discussion
It has been previously described that with the efficacy of antimicrobial preservation test inoculum simple syrup was microbiologically stable for 14 days [6] which corresponds to the results we found with both maltitol and glucose unpreserved syrups. It may be therefore interesting to start compounding unlicensed paediatric drugs with these unpreserved syrups if you are looking for a 15-days stability without adding preservatives though the efficacy of antimicrobial preservation remains to be performed in the presence of the selected API.
The same study also found that simple syrup failed to meet European Pharmacopoeia acceptance criteria with A. brasiliensis at day 28 which also corresponds to the results we found with both maltitol and glucose unpreserved syrups. Indeed, A. brasiliensis is a xerophilic species, which can live in a water-poor environment. It can germinate in an

Candida albicans
Liquid glucose Sample

Aspergillus brasiliensis
Liquid glucose Sample  *Indicate an increase in comparison to the previous count. UFC, unit forming colony.
Carpentier et al.: Preservation efficacy of glucose and maltitol syrups environment with a very low water activity [12] which explains why osmotic pressure alone cannot inhibit the growth of Aspergillus in selected syrups. A study on the contamination of maple syrups [13] also found that Apergillus species were common contaminants during packaging at the minimum recommended reheating temperatures, which illustrates the ability of Aspergillus to grow in unpreserved syrups at room temperature. When stored at 4°C for 12 months, no growth was observed in maple syrups. While our studies did not explore the effect of temperature on the efficacy of antimicrobial preservation of unpreserved syrups, this should be done on final formulation to see if unpreserved syrups could meet acceptance criteria thus limiting the use of preservatives.
Preserved maltitol and glucose syrups meet acceptance criteria on day 28, with no regrowth of A. brasiliensis being observed. It is well known that sorbic acid is effective on fungi and on A. brasiliensis [14]. Therefore it seems interesting to use it rather than parabens if this species is incriminated in the failure of the antimicrobial preservation test as previously observed with simple syrups [6] or observed in our study, as it looks safer for compounding paediatric drugs.
To go further it would be interesting to study the impact of a dilution in water of syrups as dilution of simple syrup, even preserved with parabens, has previously been described as problematic [6,15]. From an industrial point of view other strains should also be tested such as Zygosaccharomyces rouxii (recommended by European pharmacopoeia) and other osmophilic yeast such as Saccharomyces cerevisae or Saccharomyces mellis [16].
Our data suggest both maltitol and glucose syrup could be used as a starting point to paediatric drug formulation notably for unlicensed drug compounding. They represent a non-cariogenic alternative to simple syrup or an alternative to commercial suspending agents. From a microbiological point of view, they are stable for at least 14 days at ambient temperature without the need to add a preservative although the impact of the selected API remains to be explored. Longer microbiological shelf life required the use of sorbic acid at a concentration of 0.1% to pass the European Pharmacopoeia test of antimicrobial preservation efficacy.