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BY-NC-ND 3.0 license Open Access Published by De Gruyter February 29, 2012

Methods of extraction and thin-layer chromatography determination of phospholipids in biological samples

Katarzyna Dyńska-Kukulska and Witold Ciesielski


Phospholipids (PLs) are a class of compounds essential for living organisms, including human beings. This paper presents a brief review concerning the extraction as well as thin-layer chromatographic separation and determination of phospholipids. After a short introduction regarding phospholipids and their specific characteristics, the extraction techniques will be discussed. Then, phospholipid separation and system detection for thin-layer chromatography (TLC) will be presented. The focus will be on phospholipids that are most abundant in biological systems; that is why chosen examples of PLs determination in biological samples will be revealed as well as general PL determination schemes. In the Summary section, there will also be a short discussion about advantages and disadvantages of PL determination with the use of TLC.

Corresponding author

Received: 2011-11-3
Accepted: 2012-2-5
Published Online: 2012-02-29
Published in Print: 2012-03-01

©2012 by Walter de Gruyter Berlin Boston

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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