This review explores the current state-of-the-art wireless and mobile optical chemical sensors and biosensors. The review is organised into three sections, each of which investigates a major class of wireless and/or mobile optical chemical sensor: (i) optical sensors integrated with a radio transmitter/transceiver, (ii) wearable optical sensors, and (iii) smartphone camera-based sensors. In each section, the specific challenges and trade-offs surrounding the (bio)chemical sensing mechanism and material architecture, miniaturisation, integration, power requirements, readout, and sensitivity are explored with detailed examples of sensor systems from the literature. The analysis of 77 original research articles published between 2007 and 2017 reveals that healthcare and medicine, environmental monitoring, food quality, and sport and fitness are the target markets for wireless and mobile optical chemical sensor systems. In particular, the current trend for personal fitness tracking is driving research into novel colourimetric wearable sensors with smartphone readout. We conclude that despite the challenges, mobile and wearable optical chemical sensor systems are set to play a major role in the sensor Internet of Things.
complementary metal oxide semiconductor
Förster resonance energy transfer
Global System for Mobile Communications
industrial, scientific and medical
lateral flow immunoassay
red, green, blue
The demand for instant (bio)chemical information at the point of measurement combined with advances in mobile communication technologies is driving the development of wireless chemical sensors and biosensors for the sensor Internet of Things. These hybrid sensing devices collect analytical data on their local (bio)chemical environment, or perform spot tests on applied samples, and process and transmit this information to a remote device wirelessly, most usually by radio communications (Farre et al. 2009, Potyrailo et al. 2011, and Ghafar-Zadeh 2015). Common radio technologies used in such systems include Bluetooth, radiofrequency identification (RFID), ZigBee, near field communication (NFC), and 433- and 860-MHz short-range devices (ISM/SRD860) (Kassal, Steinberg, and Steinberg 2018). Optical chemical sensors and biosensors lend themselves to integration into wireless systems thanks to their ease of miniaturisation, the unnecessary use of reference electrodes, the high detection sensitivity of certain modes (e.g., fluorescence), and the diversity of optically responsive indicator chemistries. In a radio-based wireless optical chemical sensor, the optical signal generated by the interaction of the analyte and receptor chemistry is converted to an electrical signal by the detector and is then passed to a remote wireless receiver or network where the data can be analysed in more detail (Figure 1A).
Wearable chemical sensing is becoming a key application area for wireless chemical sensors (Diamond et al. 2008) and has been the research focus of analytical chemists for many years. Several review papers have been published on electrochemical wearable sensors (Windmiller and Wang 2013, Bandodkar and Wang 2014, Steinberg, Kassal, and Steinberg 2016, and Malon Radha, Heng Lee, and Córcoles Emma 2017), but no similar review has yet been undertaken on optical wearable sensors. Wireless (radio-based) communication is often essential to ensure complete mobility of many types of wearable sensor, especially electrochemical devices (Steinberg, Kassal, and Steinberg 2016). However, in a wearable optical system, the output signal can also be read by the naked eye or a camera (Figure 1B). Thus, both radio and visual readout systems are receiving attention from the academic community.
One mobile platform particularly being increasingly used in (bio)chemical sensing – the smartphone – has now become ubiquitous. According to the International Telecommunication Union (ITU), mobile cellular subscriptions reached 7.4 billion globally in 2016, almost reaching the number of people on Earth (ITU 2016). This pervasiveness, as well as the increasing computational ability of smartphones, makes them an ideal mobile platform for collection and analysis of (bio)chemical data (Zhang and Liu 2016). The smartphone can function as both a readout and a data analysis unit, typically using smartphone-enabled radio standards for readout such as Bluetooth, NFC, Wi-Fi, and Global System for Mobile Communications (GSM) (Figure 1A). Meanwhile, the increasing quality of integrated cameras enables the smartphone to also serve as the detector in optical (bio)chemical sensing systems (Figure 1B).
This review explores the current state-of-the-art and recent advances in wireless optical chemical sensors and biosensors, with emphasis on three major groups of mobile (bio)chemical systems: radio-based optical chemical sensors, optical chemical sensors that are wearable, and smartphone camera-based sensors. These groups are not mutually exclusive and the overlap is explained in Section 2.
2 Methodology and structure
Academic databases from the Web of Science were searched for chemical sensors and biosensors, using combinations of keywords indicating the sensors’ wireless and mobile nature – “wireless,” “contactless,” “wearable,” and “smartphone” – encompassing different modes of optical sensing, such as “absorbance” and “fluorescence.” The initial literature search generated 281 publications (journal articles and conference proceedings) published from 2003 onwards (Figure 2). The scope of the review was then limited to research work in the area of wireless and mobile (bio)chemical sensing, which consequently reduced the number of publications to 77.
To ensure the review is both topical and current, we define mobile sensing to include wearable and smartphone-based sensors – two extremely popular topics in analytical chemistry today. The growth of research in these fields is such that we present these in their own dedicated sections (Sections 4 and 5). However, mobile, handheld, and portable devices without wireless communication were deemed out of scope and are therefore not considered in our analysis. Further reading on these latter devices can be found elsewhere (Capitán-Vallvey and Palma 2011 and Choi 2016).
The review is thus structured in three parallel sections that may be read independently, each covering a major group of wireless and/or mobile chemical sensors. First, optical chemical sensors and biosensors integrated with a radio transmitter/transceiver are presented (Section 3). Second, optical wearable chemical sensors and biosensors are presented and discussed in Section 4. Although there is some overlap here with the former grouping, we think the recent explosion in academic research on wearable sensors and their applications justifies this subdivision. Clearly, the analytical data from a wearable optical sensor can be retrieved wirelessly by radio communication (Figure 1A) but may alternatively be retrieved by the naked eye or a remote camera (Figure 1B). Accordingly, we consider both wearable optical sensors with and without wireless (radio) data transfer. Lastly, chemical sensors and biosensors in which the optical signal is detected by smartphone camera are presented and discussed (Section 5). Although there are numerous examples of publications dealing with techniques for processing and analysing digital images in computer vision-based analytical procedures (Capitán-Vallvey et al. 2015), we have focused here on novel sensor chemistries and optical arrangements. Finally, the summary findings from all 77 research publications are presented (Section 6) together with our conclusions and future outlook on the field (Section 7).
3 Wireless optical sensors
Optical chemical sensors are devices that respond to or generate optical signals when a chemical or biological analyte interacts with a receptor. In its simplest embodiment, an optical sensor might indicate the absorbance of an analyte in solution directly (or an analyte-sensitive indicator molecule), or for example, a more complex optical chemical sensor might indicate the luminescence intensity emitted by an indicator molecule in a receptor layer. In a radio-based wireless optical chemical sensor, the optical signal generated by the interaction of the analyte and receptor chemistry is converted to an electrical signal by the detector and is then passed to a remote wireless receiver or network where the data can be analysed in more detail (Kassal, Steinberg, and Steinberg 2018).
In this section, we present a review of current academic research and development on optical chemical sensors and biosensors integrated with radio transceivers (Figure 1A), but which are not wearable. The wearable devices are presented separately in Section 4 in acknowledgement of the recent explosive growth in wearables in the past few years.
Wireless data transfer offers a significant advantage over wired systems, especially in applications where chemical analytes need to be monitored in places that are not easily accessible or hazardous. Therefore, wireless chemical sensors have found application mainly in environmental monitoring, for distributed air and water analysis, and in healthcare, as implantable or swallowable sensors. The sensors analysed in this section have been grouped by their application. Of the 77 publications covered by this review, 17 papers (22%) were found to be based on wireless optical sensing, of which the main application areas were environmental monitoring (47%) and healthcare (24%).
3.1 Environmental monitoring
Wirelessly networked autonomous environmental analysers have been envisaged since the beginning of the millennium (Sequeira et al. 2002), and realisations of such systems quickly started to emerge. More than half of the wireless (bio)chemical sensors detected in the literature search have been developed for environmental monitoring applications, about evenly distributed between air quality and water quality monitoring (Table 1).
|Analyte||Sample||Detection mode||Detection chemistry||Immobilisation method||Wireless communication||Reference|
|pH (acetic acid vapour)||Air||Absorbance||Bromophenol blue||Ethyl cellulose||ISM/SRD||Shepherd et al. (2007)|
|pH (NH3 gas)||Air||Absorbance||3′-3″-Dichlorophenol sulfonephthalein||Sol-gel||ZigBee||Llorente-Alonso et al. (2013)|
|NO2||Air||Absorbance||Diazo coupling||Porous glass||ISM/SRD||Maruo et al. (2012)|
|Humidity||Air||Absorbance (IR)||Direct optical||–||ZigBee||Cao et al. (2014)|
|Phosphate||Water||Absorbance||Molybdovanadophosphoric acid||None – wet chemistry||GSM||Slater et al. (2007)|
|KMnO4 (model)||Water||Absorbance||Direct optical||–||Bluetooth||Camarillo-Escobedo, Valdés-Perezgasga, and Rodríguez-Rivera (2013)|
|pH||Water||Absorbance||Bromocresol purple||Ionogel film||Bluetooth||Czugala et al. (2012)|
|pH||Water||Reflectance||Universal Indicator||Paper, ethyl cellulose||Proprietary (wireless camera)||Fay et al. (2010)|
|NH3 dissolved||Water||Fluorescence||Eosin, pH-sensitive fluorophore||Glass substrate||Xbee||Deng et al. (2016)|
3.1.1 Air quality
An early example of a wireless air quality sensor was an absorbance-based pH sensor for environmental acidity monitoring, which detected acetic acid plumes as model analyte (Shepherd et al. 2007). The system used a paired emitter – detector diode (PEDD) setup. In this setup, the light source is an LED and the detector is the same type of LED, but reverse biased. The photocurrent is measured indirectly by monitoring the discharge time of the detector in microseconds. Both LEDs were coated with ethyl cellulose doped with bromophenol blue (a pH indicator dye). The sensors were integrated with Crossbow wireless motes (ISM/SRD860), and several sensors were deployed to monitor plume movement. A more recent example for environmental acidity was developed by Llorente-Alonso et al. (2013) and tested with NH3 vapour. The sensor chemistry was developed by entrapping a pH indicator dye (3′-3″-dichlorophenol sulfonephthalein) within a sol-gel silicate film. The sensor uses an LED source and photodiode detector, and is accurate within 0.1 pH units. It is integrated with a ZigBee module and should be applied to cultural heritage conservation.
Maruo et al. (2012) have developed a wide-area ubiquitous network based on Crossbow motes (ISM/SRD860) in which bicycles equipped with mobile sensors collect NO2 concentration data in urban areas and transmit it wirelessly. The sensing element was porous glass doped with diazo coupling reagents, which selectively react with NO2 to produce an azo dye, the absorbance of which was measured with an LED and photodiode. The mobile sensors were equipped with a GPS unit that enabled the development of air quality maps, which could be sent directly to cyclists’ mobile phones. The work of Cao et al. (2014) presents an example of a wireless chemical sensor that uses a laser source. The wireless humidity sensor represents a node in a ZigBee network and uses an IR laser diode and InGaAs detector to measure the infrared fingerprint of water. Apart from humidity, the system can be used for other gases. The sensor exhibits good linearity, stability, and selectivity, but the power consumption was not discussed.
3.1.2 Water quality
One of the earliest wireless water quality analysers was an autonomous field-deployable device for phosphate monitoring (Slater et al. 2007). Phosphate measurement was based on a microfluidic chip that mixes the reagent (molybdovanadophosphoric acid) and sample, after which the absorbance of the produced complex is measured with a LED and photodiode. The system was fairly large because it included reagent bottles, waste bottles, pumps, electronics, and a large battery; and power consumption was identified as a major issue. A GSM modem was used for wireless communication, exploiting the extensive coverage of the GSM network in Europe at the time. Collected data could also be sent in SMS messages to save power.
Camarillo-Escobedo, Valdés-Perezgasga, and Rodríguez-Rivera (2013) developed an autonomous optical microanalyser (μTAS) based on Bluetooth. Common pumps and injection valves were replaced by piezoelectric micropumps and microvalves, which bring the developed system closer to the autonomy and portability needed for real-time and in situ analysis. This work significantly reduced the reagent consumption, sample consumption, and waste generation, making a step towards green chemistry. The analyser can perform 2640 sampling cycles on a Li-ion battery. The system was tested with KMnO4 solutions, and a good comparison against a reference spectrophotometer was achieved. Czugala et al. (2012) developed a centrifugal portable system for in situ colourimetric analysis of water pH (Figure 3A). The system uses a PEDD setup, where an ionogel film, doped with bromocresol purple, is placed between the two diodes. Centrifugal disc technology removes the need for pumps, microfluidic interconnections, and fluid handling systems and can process multiple samples simultaneously. Bluetooth was used for wireless communication. A good comparison against a benchtop spectrophotometer was achieved.
A different approach to remote water quality monitoring has been demonstrated by Fay et al. (2010), who have developed a biomimetic robotic fish that can gather water pH data from multiple distributed sensing stations. The stations are equipped with a universal indicator strip, coated with ethyl cellulose to prevent leaching. The robotic fish has an on-board wireless camera that records colour of the stations and sends this information to a remote base station where local pH can be calculated with image processing software. Deng et al. (2016) have developed a fluorescence-based environmental sensor for dissolved ammonia (Figure 3B). The sensor uses eosin as the pH-sensitive fluorescent dye that is immobilised on a glass substrate by a gas-permeable protection layer. Localised increases in ammonia concentration increase eosin fluorescence, which is detected with an LED and photodiode and transmitted to a base station using an Xbee module (proprietary radio module based on the IEEE 802.15.4 protocol).
The second largest application area of wireless chemical sensors is healthcare. The wireless optical sensors found in this group were highly miniaturised and mostly intended to be used as implantable and swallowable sensors. The main features of the sensors are summarised in Table 2, along with examples from other application areas (discussed in Section 3.3).
|Analyte||Sample||Detection mode||Detection chemistry||Immobilisation method||Wireless communication||Application||Reference|
|Glucose||Interstitial fluid||Fluorescence||Bis-boronic acid fluorescent indicator||Hydrogel||NFC and Bluetooth||Healthcare||Mortellaro and DeHennis (2014)|
|Glucose||Interstitial fluid||Fluorescence||Fluorescent-labelled analyte binding protein||Hydrogel||ZigBee||Healthcare||Valdastri et al. (2011)|
|Fluorescein||Blood||Fluorescence||Direct optical||–||ZigBee||Healthcare||Nemiroski et al. (2015)|
|TSH||–||Chemiluminescence||TSH antibody||–||RFID||Healthcare||Yazawa et al. (2014)|
|pH||–||Reflectance||Bromocresol green||Sol-gel||RFID||General purpose||Steinberg and Steinberg (2009)|
|K+, model dye (NB)||Aqeous solution||Absorbance||Optode: valinomycin, N-octadecanoyl-Nile blue||PVC optode, spin-coating||RFID||General purpose||Steinberg et al. (2014)|
|O2||Ambient gas||Fluorescence||PtOEP||Polystyrene, spin-coating||RFID||Food quality||Martinez-Olmos et al. (2013)|
|Glucose||–||Refractive index (Fabry– Pérot)||Direct optical||–||ISM/SRD||General purpose||Pang et al. (2012)|
PVC, polyvinyl chloride.
Mortellaro and DeHennis (2014) have described a subcutaneously implanted glucose sensor that uses a fluorescent, nonenzymatic glucose indicating hydrogel and a miniaturised optical detection system (Figure 4A). A fluorescent bis-boronic acid derivative is immobilised in the hydrogel (pHEMA). Upon binding of glucose, there is a disruption of the intramolecular photo-induced electron transfer, which causes increased fluorescence intensity. This change in fluorescence is detected with a UV LED and two photodiodes. The sensor is passive – it is powered by and communicates with a body-worn reader via NFC. The reader is equipped with a Bluetooth module that transmits over longer distances, and it has a battery that can simply be recharged at will. The sensor is not subject to the stability limitations inherent to enzyme-based systems and is less affected by biofouling than electrochemical sensors because the signal is derived from the entire bulk of the hydrogel and not just the surface. The wireless sensor was implanted and tested in vivo during a period of 28 days. Another example of an implantable fluorescent-based glucose sensor has been demonstrated in the literature (Valdastri et al. 2011). It contains a hydrogel with immobilised analyte binding protein covalently linked to two fluorescent proteins. Analyte-induced changes in FRET between two fluorescent proteins are detected with a laser diode source and phototransistor detectors. Because of the large power consumption of the laser diode, half of the volume of this implantable sensor is occupied by a battery, which hinders possibility of implantation.
A swallowable wireless capsule containing a miniature fluorimeter has been developed to monitor gastrointestinal bleeding (Figure 4B) (Nemiroski et al. 2015). The fluorimeter detects the presence of a leaked fluorescent dye (fluorescein), previously injected into the blood stream, in the gastrointestinal tract. Bulky focusing optics were eliminated by using pinholes to form a 0.2-μL detection volume in which 20 nM of fluorescein could be detected. The capsule transmits data to an external unit via the ZigBee protocol and can operate for up to several days, depending on the measurement and transmission rates. Lastly, an example of a wireless biosensor using chemiluminescence was demonstrated (Yazawa et al. 2014). The system is based on a passive RFID tag with an integrated photodiode that measures luminescence in a microfluidic channel. The sensor was used to detect thyroid-stimulating hormone (TSH) in a sandwich microfluidic immunoassay. The sensor itself consumes very little power, but the microfluidics are operated separately; and the measurement procedure is very long and uses several reagent additions and washing steps.
3.3 Other applications
A highly integrated passive RFID platform using dual-wavelength optical absorption for chemical sensing was introduced by Steinberg and Steinberg (2009) (Figure 5A). The system uses two LEDs and a photodiode in a planar configuration. The sensing chemistry used in the proof-of-concept demonstration was a sol-gel silicate thin film with an immobilised pH-sensitive dye bromocresol green. A good correlation of the pH response against a laboratory spectrophotometer was demonstrated in the 5.2–8.3 range. The performance of the optical RFID tag was tested further in absorptiometric (transmission) mode, in two model analytical applications: photometry in solution, where dye concentration was determined, and potassium sensing with valinomycin-based bulk optode membranes (Steinberg et al. 2014). In both cases, the results correlated well with those obtained with a reference spectrophotometer.
Martinez-Olmos et al. (2013) developed a passive RFID-based fluorescent O2 sensor for monitoring the freshness of packaged food (Figure 5B). The RFID antenna was created by screen printing on one side of a polyester substrate; and the other electronic components, including an UV LED source and RGB detector, were added to the same side. A polystyrene film containing platinum octaethylporphyrin (PtOEP) was placed on the other side. Fluorescence of PtOEP is quenched by oxygen that can be detected through the polyester packaging. The system exhibited a resolution of 0.1 ppm, an accuracy better than 0.1%, and an LOD of 40 ppm.
Pang et al. (2012) have developed an optical wireless sensor network, based on fiberoptic Fabry–Pérot sensors and Crossbow motes. The multisensor platform consisted primarily of optical physical sensors (temperature and pressure sensors), but chemical sensing (through refractive index changes of the sample solution) was also demonstrated with glucose as the model analyte.
4 Wearable optical sensors
Wearable chemical sensors represent a specific subgroup of wireless sensors. Their application requires miniaturisation and integration into wearable accessories, into clothing, and into body art as wearables for personal biomonitoring and personal microenvironment monitoring (Steinberg, Kassal, and Steinberg 2016).
As mentioned in the Introduction, wearable sensors have early been identified as a major application area of wireless chemical sensors (Diamond et al. 2008). In recent years, wearable (bio)chemical sensors have become very popular because they enable real-time and noninvasive monitoring of a multitude of healthcare- and fitness-associated analytes (Bandodkar and Wang 2014 and Matzeu, Florea, and Diamond 2015). Although a significant amount of wearable electrochemical sensors is present in the literature, optical detection in wearable systems is less popular. The main reason for this might be the need for the light source and detector, which are rigid components and are challenging to implement into a wearable system (Windmiller and Wang 2013). Nevertheless, optical wearable sensors are present in the literature and are discussed here. Of the 77 publications covered by this review, 17 papers (22%) could be classified as wearable optical sensors. Of the wearable sensors, 29% had integrated electronics for radio communication. The main application areas of wearable sensors were found to be sport and fitness (41%) and healthcare (35%).
4.1 Wearable optical sensors for sport and fitness applications
A particularly promising area for wearable chemical sensing is real-time perspiration analysis (Steinberg, Kassal, and Steinberg 2016). Sweat is easily accessible and contains multiple analytes that are important for sport physiology (Bandodkar and Wang 2014). Therefore, all of the wearable sensors discussed in this section use sweat as the sample. Other features of the wearable sensors for sweat sensing are summarised in Table 3.
Caldara et al. (2012) have developed a textile-based wearable optical pH sensor. The sensor consists of cotton fabric with a pH indicator methyl red immobilised in a hybrid sol-gel (ormosil) matrix. The miniaturised wearable electronics included colour detection optoelectronics (white LED and photodiode) and microcontroller. The sensor exhibits low power consumption and resolution of 0.05 pH units with dynamic range from pH 4 to 8, which is suitable for sweat pH analysis. The system was improved by covalent immobilisation of litmus as a pH indicator into a hybrid sol-gel matrix and using Bluetooth for wireless data transfer (Caldara et al. 2016). The authors emphasise much shorter times for colour transition in comparison to methyl red dye used in previous work. On-body pH measurements during physical activity were carried out. The operational lifetime was determined to be 24 h while sampling every 5 s and transmitting data every 2 min, which is adequate for physical activity monitoring. A textile-based sensor incorporating a microfluidic platform with passive pumping and channels for collecting sweat was developed by Morris et al. (2009). The sweat pH measurements were carried out by paired emitter-detector LEDs, where the LEDs were at an angle and measured reflectance from a pH-sensitive layer with immobilised bromocresol purple. On-body trials of sweat pH during physical exercise were performed using a waistband and a Crossbow mote enabled wireless communication. The system was optimised by substituting the LED detector with a photodiode and operating in transmission mode to increase sensitivity, improving the microfluidics by introducing a cotton thread to ensure fast and homogeneous flow, and by using Bluetooth communication (Curto et al. 2012) (Figure 6A).
|Analyte||Sample||Detection mode||Detection chemistry||Immobilisation method||Wireless communication||Application||Reference|
|pH||Sweat||Reflectance||Methyl red||Hybrid film on cotton fabric||None||Sport and fitness||Caldara et al. (2012)|
|pH||Sweat||Reflectance||Litmus||Sol-gel ormosil (GLYMO); cotton fabric immersed||Bluetooth||Sport and fitness||Caldara et al. (2016)|
|pH||Sweat||Reflectance||Bromocresol purple||Dye co-immobilised with tetraoctylammonium bromide on textile||ISM/SRD||Sport and fitness||Morris et al. (2009)|
|pH||Sweat||Reflectance||Bromcresol purple||Immobilisation on textile||Bluetooth||Sport and fitness||Curto et al. (2012)|
|Lactate||Sweat||Reflectance||Oxidase enzyme and ABTS||Photolithography on textile||None||Sport and fitness||Baysal et al. (2014)|
|pH||Sweat||Silicone||Reflectance (RGB imaging)||pH indicators||Silicone substrate doped with pH indicator||Fitness and healthcare||Huang et al. (2014)|
|pH, chloride, lactate||Sweat||Reflectance (RGB imaging)||Enzymes, 2,4,6-tris(2-pyridyl)-s-triazine and pH indicator||Lithographic technique||NFC||Sport and fitness, healthcare||Koh et al. (2016)|
Baysal et al. (2014) presented a textile-based sensor for lactate analysis in sweat. An absorbent biocompatible nonwoven substrate was used for fabrication and design of microchannels and reservoirs as well as hydrophobic barriers by photolithography. The obtained porous structure resulted in capillary flow of sweat. Lactate concentration was determined by an oxidase enzyme-specific reaction. Two enzymes were added to the reservoir, namely, lactate oxidase and peroxidase and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). Oxidation of lactic acid produces hydrogen peroxide that leads to oxidation of ABTS, resulting in change of colour from green to brown. Huang et al. (2014) designed stretchable and wireless electrical (dielectric) sensors for volumetric sweat measurement with the possibility of both colourimetric and electrical analysis. Silicone substrates with immobilised pH indicator dyes show visible colour change in buffer solutions of different pH. The authors also demonstrated the detection of iron and copper ions with commercial colourimetric kits. Koh et al. (2016) went a step further and developed a soft, flexible, and stretchable microfluidic system for simultaneous detection of four analytes in sweat: pH, chloride, lactate, and glucose, as well as sweat volume and sweat rate (Figure 6B). This microfluidic platform is composed of three parts: (i) an adhesive layer with openings for sweat capture, (ii) a complement of channels and reservoirs with reagents for paper-based colourimetric analysis, and (iii) electronics that include a magnetic loop antenna for wireless NFC. Sweat is captured in a set of microchannels and reservoirs that use capillary action and natural pressure to move towards separate regions for analysis. The smartphone camera is initialised automatically when near the device using NFC and captures a digital image. On-body human trials proved high biocompatibility with no discomfort or skin irritation.
4.2 Wearable optical sensors for healthcare and other applications
4.2.1 Wound monitoring
Wound monitoring has been identified as a key application area of wearable sensors in healthcare (Steinberg, Kassal, and Steinberg 2016). In wound monitoring scenarios, wireless data transfer should enable truly noninvasive wound assessment, which is of paramount importance in reducing patient discomfort (Mehmood et al. 2014). pH of wound fluid is an important wound status biomarker, and all of the optical wound sensors found in the literature detect wound pH as the analyte (Table 4).
Pasche et al. (2009) have developed a sensor with pH-responsive hydrogels and covalently immobilised phosphorylcholine receptors incorporated into optical fibers for detection of pH and C-reactive protein concentration in wound. Surface functionalisation of the fiber was obtained using a photoactive dextran-based coating (OptoDex®) [Arrayon Biotechnology (Neuchâtel, Switzerland)] covalently bound on to the surface, enabling specific covalent attachment of the target biomolecule. The portable sensing system contains optical fibers connected to the light source (LED) and the detector, which allows monitoring of the change in the refractive index at the surface of the fiber. Poly(methyl methacrylate) (PMMA) fibers show great potential for use in textile-based sensing systems as they can be woven into textiles. Schyrr et al. (2014) have developed a mobile optical fiber sensor for pH wound measurement. They obtained the sensor by coating PMMA optical fibers with a sol-gel film containing a pH-sensitive dye. pH detection was provided with portable sensing device with light coupler and LED measuring light absorption variation in the coating. The sensor was tested in blood serum samples and manifested reversibility, a precision of 0.2 pH units, and a response time of 20 min.
We have reported on a wireless smart bandage for optical wound pH detection (Figure 7A) (Kassal et al. 2017). A pH indicator dye was covalently bound to cellulose particles dispersed within a biocompatible hydrogel, which enables production of pH-sensitive layers on different kinds of dressing materials. Detection was provided with an optoelectronic probe and a miniature wireless platform that measures and stores pH data and transfers them to a remote computer using RFID. The results show high accuracy and precision of the smart bandage in the relevant pH range and could provide insight into temporal pH changes of wounds during the healing process. Tamayol et al. (2016) have developed hydrogel fibers for epidermal pH detection. pH-responsive microbeads, loaded with brilliant yellow, were dispersed within the alginate-based microfibers using a microfluidic spinning method that enabled manipulation of the fiber size. The produced fibers were used as a medical tape-based wound dressing and the pH was measured via smartphone image capture.
|Analyte||Sample||Substrate||Detection mode||Detection chemistry||Immobilisation method||Application||Reference|
|pH, C-reactive protein||Wound fluid||Waveguide||Refractive index||pH responsive hydrogel||Hydrogel, spin-coating||Healthcare||Pasche et al. (2009)|
|pH||Wound fluid||PMMA optical fibers||Absorbance||Bromphenol blue||Sol-gel||Healthcare||Schyrr et al. (2014)|
|pH||Wound fluid||Cotton bandage||Reflectance||4-[4-(2-Hydroxyethanesulfonyl)-phenylazo]-2,6-dimethoxyphenol||Cellulose particles, dispersed in hydrogel||Healthcare||Kassal et al. (2017)|
|pH||Wound fluid||Alginate hydrogel||Reflectance||Brilliant yellow||Alginate microfibers||Healthcare||Tamayol et al. (2016)|
|Blood||Gastrointestinal tract||Bioadhesive polymer||Reflectance||PPG sensor||Mucoadhesive films based on carbopol-based hydrogel||Healthcare||Chan et al. (2015)|
|pH||Aqueous solution||PVC||Reflectance||Bromothymol blue, bromocresol green, cresol red||Physical entrapment into PVC matrix||General purpose||Kim et al. (2016)|
|Ammonia gas||Gas||PET||Reflectance||Bromophenol blue||R-GO coupled with organic dye molecules||Safety||Duy et al. (2016)|
|Cocaine||Sweat||Paper||Fluorescence||Dylight649||Immobilisation on paper||Forensic||Walczak, Kruger, and Moynihan (2015)|
|pH||Aqueous solution and vapour||Paper, textile, TLC alumina and silica plates||Reflectance (RGB imaging)||12 pH indicators||Glass microfiber filter papers, cotton threads, silica TLC||Healthcare||Galpothdeniya et al. (2014)|
|Thiourea, cyanide, iodide, methimazole||Unspecified||Paper||Reflectance||Au NP deposited on bacterial cellulose nanopaper and nitrocellulose||Embedded silver and gold nanoparticles||General purpose||Morales-Narváez et al. (2015)|
R-GO, reduced graphene oxide.
4.2.2 Other applications
Wearable sensors can also be used for in vivo monitoring with an “in-body wearable system,” as demonstrated by Chan et al. (2015). Bio- or mucoadhesive anchoring is a challenge for the development of advanced gastrointestinal surgical instruments, endoluminal monitoring devices, and drug delivery systems. The authors presented a proof of concept of an endoluminal photoplethysmographic (PPG) sensing patch with bioadhesive polymers for the detection of gastrointestinal bleeding by direct optical absorption of LED light. The authors synthesised four different mucoadhesive films based on a biocompatible carbopol hydrogel. The PPG sensor was placed on a flexible circuit board and built in between two muchoadhesive films. The authors’ findings demonstrate a potential for development of in vivo wearable systems. Wearable sensing goes a step further with using fingernails as a platform for sensors. Kim et al. (2016) have designed a colourimetric pH sensor on a polyvinyl chloride matrix in the shape of a fingernail. To expand the range of pH sensing, they used three different pH indicators: bromothymol blue, bromocresol green, and cresol red. The sensors provide reversible, repeatable, and fast response with an intense colour change. Such a wearable platform can be used for analysis of different samples. Duy et al. (2016) have developed a flexible and transparent chemical sensor for colourimetric and electrical sensing of ammonia gas (5–40 ppm). The sensor consists of reduced graphene oxide with pH indicator dye bromophenol blue on a PET substrate. When the sensor is exposed to ammonia gas, it visually changes colour depending on humidity conditions, and this can be detected using a smartphone camera. In contrast, the resistance of the device increases in the presence of ammonia and can be measured. Visual detection shows a very fast response time (<15 s) compared with electrical detection (15–20 min). However, visual mode has a limited working range and needs humid conditions.
Walczak, Kruger, and Moynihan (2015) have developed a test-strip fluorescent sensor for detection of cocaine in sweat. The lateral flow strip consists of a control line and test line, and recognition is based on a competitive immunoassay approach. A handheld fluorescence reader was used for concentration measurement (Figure 7B). Galpothdeniya et al. (2014) developed ionic liquid (IL)-based thread optoelectronic sensor arrays for pH detection in aqueous solutions. Twelve different sensors were fabricated by an ion exchange reaction between anion pH indicator dyes and cation trihexyl(tetradecyl)phosphonium. The synthesised IL showed low solubility in aqueous solutions. The authors immobilised IL sensors into four different matrices [glass microfiber filter papers, cotton thread, silica thin layer chromatography (TLC) plates, and alumina TLC plates] and used them for the colourimetric pH analysis of aqueous solutions and the detection of acidic and basic vapours. The results show that the alumina matrix is a good choice for acidic vapour sensing, and the silica matrix for basic vapour sensing. Thread-based sensor arrays have a great potential for application in the fabrication of wearable sensing because of its regeneration and reuse, as well as flexibility, small sample volume requirement, and low weight. Morales-Narváez et al. (2015) have reported on nanopaper-based platforms for sensors with plasmonic or photoluminescent properties. The platforms developed include a colourimetric sensor based on nanopaper containing embedded silver and gold nanoparticles, a photoluminescent sensor with CdSe@ZnS quantum dots conjugated to nanopaper, and a potential up-conversion sensing platform constructed from different nanoparticles. The authors indicate that the designed platforms can show high selectivity and specificity through functionalisation with biomolecules and they can work in complex matrixes.
5 Smartphone camera-based optical sensors
Today, the smartphone is near ubiquitous, and thanks to powerful processing capabilities, can function as both a readout and a data analysis unit, typically using standard radio protocols for readout such as Bluetooth, NFC, Wi-Fi, and GSM (Figure 1A). Meanwhile, the increasing quality of integrated cameras enables the smartphone to also serve as the detector in optical (bio)chemical sensing systems (Figure 1B).
Smartphones are an excellent mobile platform for optical sensing applications because of their integrated camera modules (focusing lens and CMOS sensor) and LED light illumination, as well as their ubiquity. The role of smartphone-based sensors and biosensors can be crucial in healthcare and environmental monitoring, security, or food quality control (Preechaburana, Suska, and Filippini 2014, McCracken and Yoon 2016, and Roda et al. 2016). In this section, we discuss chemical sensors and biosensors in which the optical signal is detected by smartphone-integrated physical sensors (almost exclusively the smartphone camera), as depicted in Figure 1B. The section is divided into three subsections based on key application areas of smartphone-based sensors – healthcare, environmental, and food quality monitoring. Of 77 publications covered by this review, 42 papers (55%) were found to be smartphone-based optical sensors. The most commonly investigated group of smartphone-based optical sensors are systems designed for healthcare and point-of-care applications (43%), then for environmental monitoring (40%) and food quality (17%).
Advancement in the use of the smartphone camera is probably most developed in smartphone-based fluorescence microscopy for imaging of various fluorescently labelled specimens in the field (Wei et al. 2013 and Contreras-Naranjo, Wei, and Ozcan 2016). Different smartphone microscopy setups have been reported, highlighting the potential for simplification and acceleration of many healthcare and point-of care applications. Another example of smartphone camera use is smartphone-based spectroscopy, which can easily replace expensive and bulky laboratory spectrometers. Recently reported smartphone spectrometers are mostly based on colourimetric (absorption or reflection) (Hu, Chen, and Sonkusale 2015 and Wang et al. 2016a), fluorescence (Yu, Tan, and Cunningham 2014) or photonic crystal-based (Gallegos et al. 2013) detection. Several groups, however, presented smartphone sensing applications based on surface plasmon resonance (de Souza, Lima, and Neff 2014 and Guner et al. 2017) and successfully presented multichannel analyses (Wang et al. 2017).
5.1 Healthcare and point-of-care diagnostics
The most commonly investigated group of smartphone-based optical sensors are systems designed for healthcare and point-of-care applications (42.5%) (Table 5). Adaptation of sensing systems for monitoring human health and application of such portable and integrated devices in medical diagnostics is extremely captivating. Telemedicine is rapidly advancing because of scientific studies on using smartphone functions in the development of novel accurate and sensitive diagnostic devices (Martinez et al. 2008). Built-in sensors, diverse applications, and constant connection through wireless data service make smartphones ideal point-of-care devices. Smartphone-based biosensing is demonstrated in different point-of-care diagnostic tests such as markers for HIV and hepatitis (Giavazzi et al. 2014), ovarian cancer biomarker (Wang et al. 2011), creatinine in urine samples (Debus et al. 2015), or bacteria in blood (Choi et al. 2016) and urine (Cho et al. 2015), showing that smartphone-based point-of-care diagnostics can become a broadly applied biotechnological tool. An easy-to-use point-of-care device with a potential commercial application is demonstrated for the quantification of cholesterol (Oncescu, Mancuso, and Erickson 2014), an alarming parameter important for preventing heart disease. Colourimetric quantification of cholesterol is enabled by enzymatic reactions on dry reagent strips. Reagents convert total cholesterol finally to quinoneimine dyes, which give the colourimetric response.
|Analyte||Sample||Smartphone integration||Detection mode||Detection chemistry||Immobilisation method||Reference|
|Ovarian cancer HE4 biomarker||Urine||Stand alone||Colourimetric||Antibodies, HRP, tetramethylbenzidine||ELISA microchip||Wang et al. (2011)|
|Protein||Ambient Solution||Stand alone||Photonic crystals transmission||Label-free analyte binding protein||Photonic crystal||Gallegos et al. (2013)|
|pH||Sweat and saliva||Additional hardware||Colourimetric (RGB imaging)||pH Indicators||Plastic strip||Oncescu, O’Dell, and Erickson (2013)|
|Lactate||Sweat and oral fluid||Additional hardware||Chemiluminescence||Luminol||Nitrocellulose membrane||Roda et al. (2014)|
|Glucose||Serum||Stand alone||Colourimetric (RGB imaging)||Glucose oxidase and HRP||Paper||Chun et al. (2014)|
|Cholesterol||Blood||Stand alone||Colourimetric (RGB imaging)||Enzymatic||Dry reagent strips||Oncescu, Mancuso, and Erickson (2014)|
|Markers for HIV and hepatitis||Serum||Additional hardware||Colourimetric (RGB imaging)||IgG antibodies||Fluorinated polymer materials||Giavazzi et al. (2014)|
|BCA||Aqueous solution||Additional hardware||Colourimetric (RGB imaging)||–||–||Su et al. (2015)|
|Cortisol||Saliva||Additional hardware||Chemiluminescence||Luminol||–||Zangheri et al. (2015)|
|Creatinine||Urine||Stand alone||Colourimetric (RGB imaging)||Picric acid||–||Debus et al. (2015)|
|Glucose||Urine||Stand alone||Colourimetric (RGB imaging)||pH indicators||Sol-gel||Jia et al. (2015)|
|uCTX-II (marker for osteoarthritis)||N/A||Stand alone||Light intensity||HRP-conjugated uCTX-II antibody||PDMS||Park et al. (2015)|
|Glucose||Blood||Stand alone||Photon detection technique||Glucose oxidase||AFSiO2 nanoparticles||Devadhasan and Kim (2015a)|
|Glucose||Saliva||Stand alone||Colourimetric (RGB imaging)||Glucose oxidase and pH indicator||Paper||Soni and Jha (2015)|
|E. coli, Neisseria gonorrhoeae||Urine||Stand alone||Light scattering||Anti-E. coli or anti-N. gonorrhoeae antibodies||Paper||Cho et al. (2015)|
|Glucose and lactate||Cell culture||Additional hardware||Colourimetric||Oxidase and HRP||Paper||Im et al. (2016)|
|E. coli||Blood||Stand alone||Colourimetric||Au NPs||Paper||Choi et al. (2016)|
|Glucose and human cardiac troponin I||N/A||Additional hardware||Colourimetric||Au NPs||–||Wang et al. (2016a)|
|Protein A human cancer biomarker||N/A||Additional hardware||Colourimetric||Biotinylated antibody||Microplate wells||Wang et al. (2017)|
BCA, bicinchoninic acid; HRP, horseradish peroxidase.
Smartphone optical sensors and biosensors for point-of-care applications mostly rely on embedded cameras for RGB imaging. However, there is an example of using other built-in sensors, such as the smartphone light meter, in biosensing of osteoarthritis marker (Park et al. 2015). The immunoblotting assay resulted in a precipitation reaction, which caused changes in light intensity. The integrated illumination sensor was used as a signal transducer, whereas changes in LED intensity passing through the sensing channel (polymer film) were numerically quantified using the lux meter application. Different techniques in smartphone optical sensing are presented in Figure 8.
Amongst all point-of-care devices, glucose monitoring systems are the most widespread. The large number of diabetic patients and the need for self-monitoring blood glucose lead to the development of a large number of point-of-care glucose sensing devices. Unlike many electrochemical sensors, optical sensors for glucose determination are based on a noninvasive and painless mechanism of sampling and detection. Glucose sensing is adapted for smartphone detection by immobilising glucose oxidase, horseradish peroxidise, and/or a pH-sensitive dye that would change colour due to gluconic acid formation during the enzymatic reaction. Smartphone optical sensors for glucose monitoring based on simple colourimetric imaging were developed for saliva (Soni and Jha 2015), urine (Jia et al. 2015), serum (Chun et al. 2014), or blood (Devadhasan and Kim 2015a). Glucose monitoring was also demonstrated by Wang et al. (2016b), presenting a smartphone-attached spectrometer. Performance of the smartphone spectrometer was compared with a commercial plate reader, revealing applicability in biosensing. Most suitable immobilisation methods presented for glucose determination are paper-based test strips (Chun et al. 2014, Soni and Jha 2015, and Im et al. 2016), sol-gel (Jia et al. 2015), or nanoparticles (Devadhasan et al. 2015b and Wang et al. 2016a).
Point-of-care diagnostics is mostly based on easy-to-use colourimetric analyses of disposable paper- or plastic-based tests to avoid an additional setup for complicated measurements. Optode-based sensors, commonly used in sensing almost all analytes, can provide a suitable sensing platform for smartphone-based optical sensors, as presented by Awqatty et al. (2014). Basic metabolic panel quantification, such as sodium, potassium, chloride, calcium, bicarbonate, glucose, urea, and creatinine, is demonstrated by smartphone fluorescence spectrophotometry. A smartphone attachment, in the form of a custom-made case consisting of a microcontroller and an optical chamber for fluorescent measurements, enables ratiometric measurements with minimal signal interference and inaccuracy. However, a smartphone accessory does not need to be expensive or robust, as presented by Zangheri et al. (2015), who demonstrated a simple 3D-printed smartphone attachment for the detection of salivary cortisol. The biosensor is based on a chemiluminescent lateral flow immunoassay method using peroxidase–cortisol conjugate and luminol/enhancer/hydrogen peroxide for detection. It should be mentioned that chemiluminescence measurements using a smartphone with minimum optical attachments are rare and challenging because of low signals that are often seriously interfered by background fluorescence or light scattering. However, even a smartphone-based bioluminescence whole-cell toxicity biosensor has been developed by Cevenini et al. (2016). 3D-printed cartridges were developed for bioluminescence detection based on green-emitting luciferase. Dimethyl sulfoxide was used as a model toxic compound on kidney cells, and a stand-alone platform for quantitative toxicity testing was presented.
Smartphone-based monitoring of athletes’ performance via lactate detection in oral fluid and sweat is presented by Roda et al. (2014). They developed a chemiluminescent biosensor for sport and fitness applications based on lactate oxidase biosensing mechanism coupled with a luminol/H2O2/horseradish peroxidise enhancing system embedded in a 3D-printed smartphone accessory. Portable and noninvasive lactate measurement with a simple readout is one of rare examples of chemiluminescent smartphone sensors. The quantification of lactate lasted less than 5 min with detection limits of 0.1 mmol/ L in sweat, which is comparable to wearable lactate sensors (Matzeu, Florea, and Diamond 2015 and Steinberg, Kassal, and Steinberg 2016).
Sweat monitoring for athletes has also been demonstrated by Oncescu, O’Dell, and Erickson (2013) by developing an integrated smartphone accessory for pH control. Sweat pH can be correlated to sodium concentration and alarm the user to hydrate during physical exercise and avoid the risk of muscle cramps. The developed accessory is based on a slot containing disposable pH test strips for colourimetric analysis using a smartphone camera.
5.2 Environmental monitoring
Environmental issues with direct influence on healthcare and human well-being, such as water quality and bacterial outspread, are reaching alarming levels in some parts of the world. Smartphone-based optical sensors for environmental monitoring are the second most widespread group (40%). Smartphone-based environmental monitoring is demonstrated in different matrices (soil, gas, water) and for diverse analytes (pH, chlorine, bacteria, heavy metals), as summarised in Table 6. Smartphone-based water quality control is presented via pH monitoring (Hossain et al. 2015a, 2015b), nitrite (Lopez-Ruiz et al. 2014), oxygen (Xu et al. 2015), catechols (Wang et al. 2016b), chlorine (Sumriddetchkajorn, Chaitavon, and Intaravanne 2013, 2014), or bacterial detection.
pH is one of the most important analytical parameters in environmental monitoring. Smartphone cameras are often used for measuring RGB intensities of pH indicators (Devadhasan and Kim 2015a and Pavai, Orosz, and Paszternak 2016). However, light intensity requirements, the difference between smartphone devices, or accuracy of measurements are often cited as main drawbacks (Xu et al. 2015 and Roda et al. 2016). To overcome reproducibility problems, pH indicators immobilised in hydrogel polyvinyl alcohol films are demonstrated as a reliable method for accurate smartphone-based real-time pH imaging (Devadhasan et al. 2015b). pH-sensitive hydrogel films also showed a potential for chemical vapour measurements.
Gunda et al. (2014) developed a portable water quality kit based on colourimetric biochemical detection of Escherichia coli. A smartphone accessory in the form of an external optical attachment is used for fluorescence detection of Salmonella and E. coli by a lateral flow immunoassay (Rajendran, Bakthavathsalam, and Ali 2014). Bacterial detection on smartphone has also been demonstrated by a light scattering method on a ground beef sample (Liang, Park, and Yoon 2014) and urine samples (Cho et al. 2015).
Low-cost and easy-to-use environmental monitoring in field and resource-limited settings is a global need. Monitoring of contamination with trace-level toxic heavy metal ions by smartphone is presented in water samples (Wei et al. 2014) and soil (Hu, Chen, and Sonkusale 2015). Wei et al. demonstrated a smartphone-based quantification of mercury (II) ions in water samples with parts per billion level of sensitivity. A handheld platform attached to a smartphone detected mercury using a plasmonic gold nanoparticle and aptamer-based colourimetric transmission assay (Wei et al. 2014). This and other examples of opto-mechanical attachments for smartphone devices are demonstrated (Figure 9).
|Analyte||Sample||Smartphone integration||Detection mode||Detection chemistry||Immobilisation method||Reference|
|Chlorine||Water||Stand alone||Colourimetric (RGB imaging)||KI–starch||–||Sumriddetchkajorn, Chaitavon, and Intaravanne (2013)|
|Explosives||Ambient solution||Stand alone||Colourimetric (RGB imaging)||KI, creatinine, and aniline||Paper||Salles et al. (2014)|
|Hg (II)||Water||Additional hardware||Plasmonic colourimetric assay||Au NPs||–||Wei et al. (2014)|
|Chlorine||Water||Additional hardware||Colourimetric (RGB imaging)||KI–starch||–||Sumriddetchkajorn, Chaitavon, and Intaravanne (2014)|
|E. coli||Water||Additional hardware||Colourimetric||Enzymatic chromogen substrate, red-gal||Syringe filters||Gunda et al. (2014)|
|E. coli and Salmonella||Aqueous solution||Additional hardware||Fluorescence||Doped silica nanoparticles||Nitrocellulose LFIA strip||Rajendran, Bakthavathsalam, and Ali (2014)|
|pH and nitrite||Water||Stand alone||Colourimetric||pH indicators||Paper||Lopez-Ruiz et al. (2014)|
|Cr(III) and Cr(VI)||Soil||Stand alone||Colourimetric (RGB imaging)||Aggregation of functionalised Au NPs||–||Chen et al. (2015)|
|O2 and pH||Water||Stand alone||Colourimetric (RGB imaging)||Platinum tetrakis pentafluorophenyl porphin and fluorescein isothiocyanate||Chitosan||Xu et al. (2015)|
|pH||Water||Additional hardware||Fluorescence||Naphtalimide derivative||–||Hossain et al. (2015a)|
|Multiple analytes||Microtiter well plate||Stand alone||Colourimetric (RGB imaging)||pH indicators||–||Christodouleas et al. (2015)|
|pH||Ambient solution||Stand alone||Colourimetric (RGB imaging)||pH indicator||Hydrogel films encapsulation||Devadhasan et al. (2015b)|
|pH||Water||Additional hardware||Fluorescence||4-Aminonapthalimide||–||Hossain et al. (2015b)|
|Toxicity||Ambient solution||Additional hardware||Bioluminescence||Luciferin||Agar gel||Cevenini et al. (2016)|
|Catechol||Water||Stand alone||Colourimetric (RGB imaging)||pH indicators||Array plates||Wang et al. (2016b)|
|Sulfur mustard||Gas||Stand alone||Colourimetric (RGB imaging)||pH indicator stripes||Polyurethane hydrogel||Bidmanova et al. (2016)|
Smartphone-based optical sensors can also offer fast detection of chemical agents of military and terroristic significance. Test strips for rapid detection of sulfur mustard by visual readout using enzyme haloalkane dehalogenase and pH indicators enable visual detection with high potential for smartphone applications (Bidmanova et al. 2016). A similar disposable paper-based platform is presented for colourimetric explosive discrimination (Salles et al. 2014).
5.3 Food quality
Smartphones are easily engaged in quality control processes in the food industry. Smartphone cameras can effectively replace robust and expensive instruments for colourimetric analysis and offer users low-cost and easy-to-use methods of monitoring and detection, especially in resource-limited environments. Easy-to-use smartphone-based detection of bacteria or amines attributed to food spoilage is demonstrated in several scientific publications presented in Table 7 (17.5%). Optical chemical sensors in food quality control are widely applied. Integrating optical systems with smartphones for smart packaging applications (Bueno et al. 2015a) or test strips for food spoilage detection (Pavai, Orosz, and Paszternak 2016) opens up new areas of research and potential new markets.
Colourimetric measurements are the most commonly used smartphone detection method, wherein microfluidic analysis presents some advantages over other chemical principles, because of the necessary miniaturisation of smartphone-based sensor systems. Park et al. (2014) presented a disposable sensor for red wine tasting based on mimicking the human tongue and nose (Figure 10). Paper microfluidics were used to detect taste chemicals whose colourimetric response in contact with chosen pH indicators is obtained by red, green, and blue pixel intensities and principal component analysis (Liang, Park, and Yoon 2014). The authors successfully classified red wines by their sweetness and bodiness using a smartphone application. Wine quality can also be monitored through iron concentration, in which case colourimetric sensory solid polymer substrates for smartphone colourimetric detection of Fe(III) have been demonstrated (Vallejos et al. 2013).
|Analyte||Sample||Smartphone integration||Detection mode||Detection chemistry||Immobilisation method||Reference|
|Iron||Wine||Stand alone||Colourimetric (RGB imaging)||8-Hydroxyquinoline||Gel-like polymer network||Vallejos et al. (2013)|
|E. coli||Meat||Stand alone||Light scattering||–||–||Liang, Park, and Yoon (2014)|
|Mixed analytes||Red wine||Stand alone||Colourimetric (RGB imaging)||pH indicators||Paper||Park et al. (2014)|
|Multiple biomarkers||Milk||Additional hardware||Fluorescence||Quantum dots labelling||Microarray chip||Ludwig et al. (2015)|
|Amine||Meat||Stand alone||Colourimetric (RGB imaging)||pH indicators||Cellulose acetate membranes||Bueno et al. (2015a)|
|Bacteria differentiation||Bacteria cell cultures||Stand alone||Colourimetric (RGB imaging)||pH indicators||Cellulose acetate membranes||Bueno et al. (2015b)|
|pH||Ambient solution||Stand alone||Colourimetric||Curcumin||Cellophane test strips||Pavai, Orosz, and Paszternak (2016)|
Colourimetric sensors mostly use built-in smartphone sensors. However, if analyte detection is based on a fluorescence signal, the smartphone device often needs an extra optical component such as light source or filters. To obtain a low-cost and low-weight smartphone accessory for fluorescence detection, Ludwig et al. (2015) developed a 3D-printed opto-mechanical attachment for detection of multiple biomarkers in milk extracts. Multiple immunoassays were designed on a microarray chip followed by labelling 48 microspots with quantum dots. QD fluorescence excited by UV light from LEDs embedded in the smartphone attachment was detected by the smartphone camera.
6 Findings of the review
Wireless and mobile optical chemical sensors and biosensors, using different modes of optical sensing – measuring absorbance, reflectance, fluorescence, chemiluminescence, refractive index, and surface plasmon resonance – are being developed through academic research. These sensors are targeted at four main application areas: healthcare and medicine (38%), environmental monitoring (31%), food quality (11%), and sport and fitness (7%) (Figure 11A). The field has seen large growth in publication productivity over several consecutive years (Figure 2) partly because of the increased popularity of smartphone-based systems and wearable systems and partly because of the increased demand for rapid point-of-measurement analytical chemistry.
Across the three groups of sensor classes we categorised here (wireless, wearable, and smartphone camera-based), pH is the most common target analyte (Figure 11B). Although pH is clearly an important analyte for many application areas (pH of wound fluid and sweat, pH of aquatic environments, air acidity, etc.), the other reason for this could be the abundance of different pH-sensitive chromophores and fluorophores that can easily be incorporated into solid-state optical sensors. Other popular analytes include glucose, bacteria, metal ions, gases (O2, NO2, and NH3), proteins, and lactate.
Power consumption of wireless sensors is one of the main issues for their successful implementation. The most power-hungry elements in an optical chemical sensor are the light source and wireless transceiver. Consequently, the most popular light sources are the ones that consume the least power – LEDs. There are examples of sensors using laser sources and superluminescent diodes, but 61% of the sensors identified in our survey use an LED source (Figure 11C). Although some colourimetric sensors use a white LED and RGB readout, detection with a single-wavelength LED and suitable photodetector is more common, most likely because of the simpler data analysis. Many sensors (mainly smartphone-based) rely on external light sources, such as ambient light, lamps, or even light from image scanners.
Smartphones themselves are an attractive platform for the development of optical sensors and biosensors, but the achievement of satisfactory analytical performance is often challenging. In the simplest form of smartphone-based sensing systems, optical signals are detected by the integrated camera in the form of an image, which is then further analysed by an analytical software running on the phone. This approach generally does not require any extra hardware attachments or accessories. However, major drawbacks such as interference of ambient light, differences between smartphone devices, and semiquantitative analyses must be overcome. It is important to mention that differences between smartphones and their integrated cameras and the hardware and low-level software used to control them are highly significant. Images acquired by smartphone camera need to look natural to the human eye, which does not necessarily mean that they are entirely reliable or accurate at differentiating true red, green, and blue colours. Because of, for example, “autofocus,” “autoexposure,” and “white balance” corrections, it is often difficult to reproducibly recognise the “tones” of a certain colour (McCracken and Yoon 2016). The optical attachments are often complex and large in size, especially attachments for fluorescence measurements because of the need for extra optical components. Approximately 38% of the smartphone-based systems identified in our review incorporated some form of additional hardware to improve the optical signal detection. Nevertheless, colourimetric measurement of biochemical test strips by smartphone has shown potential for widespread use. Such point-of-care tests can be carried out without any extra attachments, outside of laboratory, and under ambient light.
Wearable optical sensors are being developed mainly on textile, paper, and polymer substrates to ensure flexibility and comfort for the end user. The focus of the research teams developing such sensors has clearly been on sweat and wound fluid analysis.
7 Conclusions and outlook
The choice of chemical sensor architecture (sensing scheme, materials, and immobilisation method) and wireless technology are application-dependent because they affect multiple parameters, such as sensitivity, limit of detection, power consumption, battery lifetime, read range, data rate, and so on. Several of the analysed wireless sensors used proprietary wireless modules, but the research focus has shifted towards standard wireless communication, namely, Bluetooth, ZigBee, RFID, and NFC. Because of the pervasiveness of smartphones, smartphone-compatible radio standards, such as Bluetooth and NFC, are becoming increasingly popular as the platform of choice for optical chemical sensor researchers.
In the future, we expect a tighter integration of the chemical sensor with miniaturised wearable platforms providing sample interface, light source, detector, data storage, processing, and radio communication, similar to the trend apparent in the wearable electrochemical sensor field. Flexible and stretchable wearable electronics, as well as advances in flexible light sources and photodetectors, could facilitate such integration. Ultimately, smartphone-integrated wireless communication could be used to retrieve analytical data from a wearable optical sensor, thus retaining complete mobility of the system while avoiding the problems associated with smartphone camera readout.
Awqatty, B.; Samaddar, S.; Cash, K. J.; Clark, H. A.; Dubach, J. M. Fluorescent sensors for the basic metabolic panel enable measurement with a smart phone device over the physiological range. Analyst2014, 139, 5230–5238.2512664910.1039/C4AN00999A Search in Google Scholar
Baysal, G.; Kok, F. N.; Trabzon, L.; Kizil, H.; Gocek, I.; Kayaoglu, B. K. Microfluidic nonwoven-based device as a potential biosensor for sweat analysis. Mechanical Design and Power Engineering. 2014, 490–491, 274–279. Search in Google Scholar
Bidmanova, S.; Steiner, M. S.; Stepen, M.; Vymazalova, K.; Gruber, M. A.; Duerkop, A.; Damborsky, J.; Prokop, Z.; Wolfbeis, O. S. Enzyme-based test strips for visual or photographic detection and quantitation of gaseous sulfur mustard. Anal. Chem. 2016, 88, 6044–6049.2711839710.1021/acs.analchem.6b01272 Search in Google Scholar
Bueno, L.; Cottell, A.; Reddy, S. M.; Paixao, T. Coupling dye-integrated polymeric membranes with smartphone detection to classify bacteria. Rsc Adv. 2015a, 5, 97962–97965.10.1039/C5RA19874G Search in Google Scholar
Bueno, L.; Meloni, G. N.; Reddy, S. M.; Paixao, T. Use of plastic-based analytical device, smartphone and chemometric tools to discriminate amines. Rsc Adv. 2015b, 5, 20148–20154.10.1039/C5RA01822F Search in Google Scholar
Caldara, M.; Colleoni, C.; Guido, E.; Re, V.; Rosace, G. Development of a textile-optoelectronic pH meter based on hybrid xerogel doped with methyl red. Sensors Actuators B Chem. 2012, 171, 1013–1021. Search in Google Scholar
Caldara, M.; Colleoni, C.; Guido, E.; Re, V.; Rosace, G. Optical monitoring of sweat pH by a textile fabric wearable sensor based on covalently bonded litmus-3-glycidoxypropyltrimethoxysilane coating. Sensors Actuators B Chem. 2016, 222, 213–220.10.1016/j.snb.2015.08.073 Search in Google Scholar
Camarillo-Escobedo, R. M.; Valdés-Perezgasga, F.; Rodríguez-Rivera, R. Micro-analyzer with optical detection and wireless communications. Sensors Actuators A Phys. 2013, 199, 181–186.10.1016/j.sna.2013.05.019 Search in Google Scholar
Cao, F.; Wang, Y.-D.; Liu, L.; Cong, M.-L. A wireless sensor network based on DFB lasers for water vapor detection. Sensors Actuators B Chem. 2014, 193, 370–374.10.1016/j.snb.2013.11.112 Search in Google Scholar
Capitán-Vallvey, L. F.; López-Ruiz, N.; Martínez-Olmos, A.; Erenas, M. M.; Palma, A. J. Recent developments in computer vision-based analytical chemistry: a tutorial review. Anal. Chim. Acta.2015, 899, 23–56.10.1016/j.aca.2015.10.00926547492 Search in Google Scholar
Cevenini, L.; Calabretta, M. M.; Tarantino, G.; Michelini, E.; Roda, A. Smartphone-interfaced 3D printed toxicity biosensor integrating bioluminescent “sentinel cells”. Sensors Actuators B Chem. 2016, 225, 249–257.10.1016/j.snb.2015.11.017 Search in Google Scholar
Chan, C. K. W.; Zheng, Y. L.; Siu, E. H. L.; Yu, R. X.; Leung, B. H. K.; Zhang, R.; Poon, C. C. Y. A mucoadhesive endoluminal wearable sensory system. 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2015, pp 4355–4358. Search in Google Scholar
Chen, W.; Cao, F.; Zheng, W.; Tian, Y.; Xianyu, Y.; Xu, P.; Zhang, W.; Wang, Z.; Deng, K.; Jiang, X. Detection of the nanomolar level of total Cr[(iii) and (vi)] by functionalized gold nanoparticles and a smartphone with the assistance of theoretical calculation models. Nanoscale. 2015, 7, 2042–2049.10.1039/C4NR06726F Search in Google Scholar
Cho, S.; Park, T. S.; Nahapetian, T. G.; Yoon, J. Y. Smartphone-based, sensitive RAD detection of urinary tract infection and gonorrhea. Biosens. Bioelectron. 2015, 74, 601–611.10.1016/j.bios.2015.07.014 Search in Google Scholar
Choi, J. R.; Hu, J.; Tang, R.; Gong, Y.; Feng, S.; Ren, H.; Wen, T.; Li, X.; Wan Abas, W. A. B.; Pingguan-Murphy, B.; Xu, F. An integrated paper-based sample-to-answer biosensor for nucleic acid testing at the point of care. Lab Chip2016, 16, 611–621.2675906210.1039/C5LC01388G Search in Google Scholar
Christodouleas, D. C.; Nemiroski, A.; Kumar, A. A.; Whitesides, G. M. Broadly available imaging devices enable high-quality low-cost photometry. Anal. Chem. 2015, 87, 9170–9178.10.1021/acs.analchem.5b0161226241835 Search in Google Scholar
Chun, H. J.; Park, Y. M.; Han, Y. D.; Jang, Y. H.; Yoon, H. C. Paper-based glucose biosensing system utilizing a smartphone as a signal reader. Biochip J. 2014, 8, 218–226.10.1007/s13206-014-8308-7 Search in Google Scholar
Contreras-Naranjo, J. C.; Wei, Q. S.; Ozcan, A. Mobile phone-based microscopy, sensing, and diagnostics. IEEE J. Sel. Top. Quantum Electron. 2016, 22, 14. Search in Google Scholar
Curto, V. F.; Coyle, S.; Byrne, R.; Angelov, N.; Diamond, D.; Benito-Lopez, F. Concept and development of an autonomous wearable micro-fluidic platform for real time pH sweat analysis. Sensors Actuators B Chem. 2012, 175, 263–270.10.1016/j.snb.2012.02.010 Search in Google Scholar
Czugala, M.; Gorkin, R., III; Phelan, T.; Gaughran, J.; Curto, V. F.; Ducree, J.; Diamond, D.; Benito-Lopez, F. Optical sensing system based on wireless paired emitter detector diode device and ionogels for lab-on-a-disc water quality analysis. Lab Chip2012, 12, 5069–5078.10.1039/c2lc40781g23070147 Search in Google Scholar
de Souza, C. A.; Lima, A. M. N.; Neff, H. Smartphone based, portable optical biosensor utilizing surface plasmon resonance. 2014 IEEE International Instrumentation and Measurement Technology Conference (I2mtc) Proceedings. 2014, 890–895. Search in Google Scholar
Debus, B.; Kirsanov, D.; Yaroshenko, I.; Sidorova, A.; Piven, A.; Legin, A. Two low-cost digital camera-based platforms for quantitative creatinine analysis in urine. Anal. Chim. Acta. 2015, 895, 71–79.10.1016/j.aca.2015.09.00726454461 Search in Google Scholar
Deng, S. J.; Doherty, W.; McAuliffe, M. A. P.; Salaj-Kosla, U.; Lewis, L.; Huyet, G. A low-cost, portable optical sensing system with wireless communication compatible of real-time and remote detection of dissolved ammonia. Photonic Sens. 2016, 6, 107–114.10.1007/s13320-016-0291-2 Search in Google Scholar
Devadhasan, J. P.; Kim, S. An ultrasensitive method of real time pH monitoring with complementary metal oxide semiconductor image sensor. Anal. Chim. Acta. 2015a, 858, 55–59.10.1016/j.aca.2014.12.015 Search in Google Scholar
Devadhasan, J. P.; Oh, H.; Choi, C. S.; Kim, S. Whole blood glucose analysis based on smartphone camera module. J. Biomed. Opt. 2015b, 20, 10. Search in Google Scholar
Duy, L. T.; Trung, T. Q.; Dang, V. Q.; Hwang, B. U.; Siddiqui, S.; Son, I. Y.; Yoon, S. K.; Chung, D. J.; Lee, N. E. Flexible transparent reduced graphene oxide sensor coupled with organic dye molecules for rapid dual-mode ammonia gas detection. Adv. Funct. Mater. 2016, 26, 4329–4338.10.1002/adfm.201505477 Search in Google Scholar
Fay, C.; Lau, K. T.; Beirne, S.; Conaire, C. O.; McGuinness, K.; Corcoran, B.; O’Connor, N. E.; Diamond, D.; McGovern, S.; Coleman, G.; Shepherd, R.; Alici, G.; Spinks, G.; Wallace, G. Wireless aquatic navigator for detection and analysis (WANDA). Sensors Actuators B Chem. 2010, 150, 425–435.10.1016/j.snb.2010.06.021 Search in Google Scholar
Gallegos, D.; Long, K. D.; Yu, H. J.; Clark, P. P.; Lin, Y. X.; George, S.; Nath, P.; Cunningham, B. T. Label-free biodetection using a smartphone. Lab Chip2013, 13, 2124–2132.10.1039/c3lc40991k23609514 Search in Google Scholar
Galpothdeniya, W. I. S.; McCarter, K. S.; De Rooy, S. L.; Regmi, B. P.; Das, S.; Hasan, F.; Tagge, A.; Warner, I. M. Ionic liquid-based optoelectronic sensor arrays for chemical detection. Rsc Adv. 2014, 4, 7225–7234.10.1039/C3RA47518B Search in Google Scholar
Giavazzi, F.; Salina, M.; Ceccarello, E.; Ilacqua, A.; Damin, F.; Sola, L.; Chiari, M.; Chini, B.; Cerbino, R.; Bellini, T.; Buscaglia, M. A fast and simple label-free immunoassay based on a smartphone. Biosens. Bioelectron. 2014, 58, 395–402.10.1016/j.bios.2014.02.07724721381 Search in Google Scholar
Gunda, N. S. K.; Naicker, S.; Shinde, S.; Kimbahune, S.; Shrivastava, S.; Mitra, S. Mobile Water Kit (MWK): a smartphone compatible low-cost water monitoring system for rapid detection of total coliform and E. coli. Anal. Methods. 2014, 6, 6236–6246.10.1039/C4AY01245C Search in Google Scholar
Guner, H.; Ozgur, E.; Kokturk, G.; Celik, M.; Esen, E.; Topal, A. E.; Ayas, S.; Uludag, Y.; Elbuken, C.; Dana, A. A smartphone based surface plasmon resonance imaging (SPRi) platform for on-site biodetection. Sensors Actuators B Chem. 2017, 239, 571–577.10.1016/j.snb.2016.08.061 Search in Google Scholar
Hossain, M. A.; Canning, J.; Ast, S.; Rutledge, P. J.; Jamalipour, A. Early warning smartphone diagnostics for water security and analysis using real-time pH mapping. Photon. Sens. 2015a, 5, 289–297.10.1007/s13320-015-0256-x Search in Google Scholar
Hossain, M. A.; Canning, J.; Ast, S.; Rutledge, P. J.; Yen, T. L.; Jamalipour, A. Lab-in-a-phone: smartphone-based portable fluorometer for pH measurements of environmental water. IEEE Sens. J. 2015b, 15, 5095–5102.10.1109/JSEN.2014.2361651 Search in Google Scholar
Hu, P. X.; Chen, Y.; Sonkusale, S. Low cost spectrometer accessory for cell phone based optical sensor. In 2015 Virtual Conference on Application of Commercial Sensors (pp. 1–5). New York: Curran Associates, Inc., 2015. Search in Google Scholar
Huang, X.; Liu, Y. H.; Chen, K. L.; Shin, W. J.; Lu, C. J.; Kong, G. W.; Patnaik, D.; Lee, S. H.; Cortes, J. F.; Rogers, J. A. Stretchable, wireless sensors and functional substrates for epidermal characterization of sweat. Small2014, 10, 3083–3090.10.1002/smll.20140048324706477 Search in Google Scholar
Im, S. H.; Kim, K. R.; Park, Y. M.; Yoon, J. H.; Hong, J. W.; Yoon, H. C. An animal cell culture monitoring system using a smartphone-mountable paper-based analytical device. Sensors Actuators B Chem. 2016, 229, 166–173.10.1016/j.snb.2016.01.121 Search in Google Scholar
ITU. Measuring the Information Society Report 2016. International Telecommunication Union, Geneva Switzerland. 2016. Search in Google Scholar
Jia, M. Y.; Wu, Q. S.; Li, H.; Zhang, Y.; Guan, Y. F.; Feng, L. The calibration of cellphone camera-based colorimetric sensor array and its application in the determination of glucose in urine. Biosens. Bioelectron. 2015, 74, 1029–1037.2627571210.1016/j.bios.2015.07.072 Search in Google Scholar
Kassal, P.; Zubak, M.; Scheipl, G.; Mohr, G. J.; Steinberg, M. D.; Steinberg, I. M. Smart bandage with wireless connectivity for optical monitoring of pH. Sensors Actuators B Chem. 2017, 246, 455–460.10.1016/j.snb.2017.02.095 Search in Google Scholar
Kim, J.; Cho, T. N.; Valdes-Ramirez, G.; Wang, J. A wearable fingernail chemical sensing platform: pH sensing at your fingertips. Talanta2016, 150, 622–628.10.1016/j.talanta.2015.12.08326838451 Search in Google Scholar
Koh, A.; Kang, D.; Xue, Y.; Lee, S.; Pielak, R. M.; Kim, J.; Hwang, T.; Min, S.; Banks, A.; Bastien, P.; Manco, M. C.; Wang, L.; Ammann, K. R.; Jang, K. I.; Won, P.; Han, S.; Ghaffari, R.; Paik, U.; Slepian, M. J.; Balooch, G.; Huang, Y. G.; Rogers, J. A. A soft, wearable microfluidic device for the capture, storage, and colorimetric sensing of sweat. Sci. Transl. Med. 2016, 8, 13. Search in Google Scholar
Liang, P. S.; Park, T. S.; Yoon, J. Y. Rapid and reagentless detection of microbial contamination within meat utilizing a smartphone-based biosensor. Sci. Rep. 2014, 4, 5953.25092261 Search in Google Scholar
Llorente-Alonso, A.; Pena-Poza, J.; de Arcas, G.; Garcia-Heras, M.; Lopez, J. M.; Villegas, M. A. Interface electronic system for measuring air acidity with optical sensors. Sensors Actuators A Phys. 2013, 194, 67–74.10.1016/j.sna.2013.01.058 Search in Google Scholar
Lopez-Ruiz, N.; Curto, V. F.; Erenas, M. M.; Benito-Lopez, F.; Diamond, D.; Palma, A. J.; Capitan-Vallvey, L. F. Smartphone-based simultaneous ph and nitrite colorimetric determination for paper microfluidic devices. Anal. Chem. 2014, 86, 9554–9562.2515812610.1021/ac5019205 Search in Google Scholar
Ludwig, S. K. J.; Tokarski, C.; Lang, S. N.; van Ginkel, L. A.; Zhu, H.; Ozcan, A.; Nielen, M. W. F. Calling biomarkers in milk using a protein microarray on your smartphone. PLoS One2015, 10, e0134360.10.1371/journal.pone.013436026308444 Search in Google Scholar
Malon Radha, S. P.; Heng Lee, Y.; Córcoles Emma, P. Recent developments in microfluidic paper-, cloth-, and thread-based electrochemical devices for analytical chemistry. Rev. Anal. Chem. 2017, 36, (4). 20160018. Search in Google Scholar
Martinez, A. W.; Phillips, S. T.; Carrilho, E.; Thomas, S. W.; Sindi, H.; Whitesides, G. M. Simple telemedicine for developing regions: camera phones and paper-based microfluidic devices for real-time, off-site diagnosis. Anal. Chem. 2008, 80, 3699–3707.1840761710.1021/ac800112r Search in Google Scholar
Martinez-Olmos, A.; Fernandez-Salmeron, J.; Lopez-Ruiz, N.; Rivadeneyra Torres, A.; Capitan-Vallvey, L. F.; Palma, A. J. Screen printed flexible radiofrequency identification tag for oxygen monitoring. Anal. Chem. 2013, 85, 11098–11105.2411637810.1021/ac4028802 Search in Google Scholar
Maruo, Y. Y.; Nakamura, M.; Higashijima, Y.; Kikuya, Y.; Nakamura, M. Development of highly sensitive nitrogen dioxide monitoring device and its application to wide-area ubiquitous network. Sensors Actuators B Chem. 2012, 173, 191–196.10.1016/j.snb.2012.06.075 Search in Google Scholar
Matzeu, G.; Florea, L.; Diamond, D. Advances in wearable chemical sensor design for monitoring biological fluids. Sensors Actuators B Chem. 2015, 211, 403–418.10.1016/j.snb.2015.01.077 Search in Google Scholar
McCracken, K. E.; Yoon, J.-Y. Recent approaches for optical smartphone sensing in resource-limited settings: a brief review. Anal. Methods2016, 8, 6591–6601.10.1039/C6AY01575A Search in Google Scholar
Mehmood, N.; Hariz, A.; Fitridge, R.; Voelcker, N. H. Applications of modern sensors and wireless technology in effective wound management. J. Biomed. Mater. Res. Part B Appl Biomater. 2014, 102, 885–895.2414251410.1002/jbm.b.33063 Search in Google Scholar
Morales-Narváez, E.; Golmohammadi, H.; Naghdi, T.; Yousefi, H.; Kostiv, U.; Horak, D.; Pourreza, N.; Merkoci, A. Nanopaper as an optical sensing platform. Acs Nano. 2015, 9, 7296–7305.10.1021/acsnano.5b0309726135050 Search in Google Scholar
Morris, D.; Coyle, S.; Wu, Y. Z.; Lau, K. T.; Wallace, G.; Diamond, D. Bio-sensing textile based patch with integrated optical detection system for sweat monitoring. Sensors Actuators B Chem. 2009, 139, 231–236.10.1016/j.snb.2009.02.032 Search in Google Scholar
Mortellaro, M.; DeHennis, A. Performance characterization of an abiotic and fluorescent-based continuous glucose monitoring system in patients with type 1 diabetes. Biosens. Bioelectron. 2014, 61, 227–231.2490608010.1016/j.bios.2014.05.022 Search in Google Scholar
Nemiroski, A.; Ryou, M.; Thompson, C. C.; Westervelt, R. M. Swallowable fluorometric capsule for wireless triage of gastrointestinal bleeding. Lab Chip2015, 15, 4479–4487.10.1039/C5LC00770D26490455 Search in Google Scholar
Oncescu, V.; O’Dell, D.; Erickson, D. Smartphone based health accessory for colorimetric detection of biomarkers in sweat and saliva. Lab Chip2013, 13, 3232–3238.2378445310.1039/c3lc50431j Search in Google Scholar
Pang, C.; Yu, M.; Zhang, X. M.; Gupta, A. K.; Bryden, K. M. Multifunctional optical MEMS sensor platform with heterogeneous fiber optic Fabry–Perot sensors for wireless sensor networks. Sensors Actuators A Phys. 2012, 188, 471–480.10.1016/j.sna.2012.03.016 Search in Google Scholar
Park, Y. M.; Han, Y. D.; Kim, K. R.; Zhang, C.; Yoon, H. C. An immunoblot-based optical biosensor for screening of osteoarthritis using a smartphone-embedded illuminometer. Anal. Methods2015, 7, 6437–6442.10.1039/C5AY01198A Search in Google Scholar
Pasche, S.; Angeloni, S.; Ischer, R.; Liley, M.; Lupranoe, J.; Voirin, G. Wearable Biosensors for Monitoring Wound Healing. In Biomedical Applications of Smart Materials, Nanotechnology and Micro/Nano Engineering. P. Vincenzini and D. DeRossi Eds.; 2009; Vol. 57; pp 80–87. Search in Google Scholar
Rajendran, V. K.; Bakthavathsalam, P.; Ali, B. M. J. Smartphone based bacterial detection using biofunctionalized fluorescent nanoparticles. Microchimica Acta. 2014, 181, 1815–1821.10.1007/s00604-014-1242-5 Search in Google Scholar
Roda, A.; Guardigli, M.; Calabria, D.; Calabretta, M. M.; Cevenini, L.; Michelini, E. A 3D-printed device for a smartphone-based chemiluminescence biosensor for lactate in oral fluid and sweat. Analyst2014, 139, 6494–6501.10.1039/C4AN01612B25343380 Search in Google Scholar
Roda, A.; Michelini, E.; Zangheri, M.; Di Fusco, M.; Calabria, D.; Simoni, P. Smartphone-based biosensors: a critical review and perspectives. TrAC Trends Anal. Chem. 2016, 79, 317–325.10.1016/j.trac.2015.10.019 Search in Google Scholar
Salles, M. O.; Meloni, G. N.; de Araujo, W. R.; Paixao, T. Explosive colorimetric discrimination using a smartphone, paper device and chemometrical approach. Anal. Methods2014, 6, 2047–2052.10.1039/C3AY41727A Search in Google Scholar
Schyrr, B.; Pasche, S.; Scolan, E.; Ischer, R.; Ferrario, D.; Porchet, J. A.; Voirin, G. Development of a polymer optical fiber pH sensor for on-body monitoring application. Sensors Actuators B Chem. 2014, 194, 238–248.10.1016/j.snb.2013.12.032 Search in Google Scholar
Shepherd, R.; Beirne, S.; Lau, K. T.; Corcoran, B.; Diamond, D. Monitoring chemical plumes in an environmental sensing chamber with a wireless chemical sensor network. Sensors Actuators B Chem. 2007, 121, 142–149.10.1016/j.snb.2006.09.045 Search in Google Scholar
Slater, C.; Cleary, J.; McGraw, C. M.; Yerazunis, W. S.; Lau, K. T.; Diamond, D. Autonomous field-deployable device for the measurement of phosphate in natural water. Advanced Environmental, Chemical, and Biological Sensing Technologies V. T. VoDinh, R. A. Lieberman and G. Gauglitz, 2007; 6755. Search in Google Scholar
Steinberg, I. M.; Steinberg, M. D. Radio-frequency tag with optoelectronic interface for distributed wireless chemical and biological sensor applications. Sensors Actuators B Chem. 2009, 138, 120–125.10.1016/j.snb.2009.02.040 Search in Google Scholar
Steinberg, M. D.; Kassal, P.; Tkalcec, B.; Steinberg, I. M. Miniaturised wireless smart tag for optical chemical analysis applications. Talanta2014, 118, 375–381.2427431110.1016/j.talanta.2013.10.033 Search in Google Scholar
Su, K.; Zou, Q.; Zhou, J.; Zou, L.; Li, H.; Wang, T.; Hu, N.; Wang, P. High-sensitive and high-efficient biochemical analysis method using a bionic electronic eye in combination with a smartphone-based colorimetric reader system. Sensors Actuators B Chem. 2015, 216, 134–140.10.1016/j.snb.2015.04.052 Search in Google Scholar
Sumriddetchkajorn, S.; Chaitavon, K.; Intaravanne, Y. Mobile device-based self-referencing colorimeter for monitoring chlorine concentration in water. Sensors Actuators B Chem. 2013, 182, 592–597.10.1016/j.snb.2013.03.080 Search in Google Scholar
Sumriddetchkajorn, S.; Chaitavon, K.; Intaravanne, Y. Mobile-platform based colorimeter for monitoring chlorine concentration in water. Sensors Actuators B Chem. 2014, 191, 561–566.10.1016/j.snb.2013.10.024 Search in Google Scholar
Tamayol, A.; Akbari, M.; Zilberman, Y.; Comotto, M.; Lesha, E.; Serex, L.; Bagherifard, S.; Chen, Y.; Fu, G. Q.; Ameri, S. K.; Ruan, W. T.; Miller, E. L.; Dokmeci, M. R.; Sonkusale, S.; Khademhosseini, A. Flexible pH-sensing hydrogel fibers for epidermal applications. Adv Healthcare Mater. 2016, 5, 711–719.10.1002/adhm.201500553 Search in Google Scholar
Valdastri, P.; Susilo, E.; Foerster, T.; Strohhoefer, C.; Menciassi, A.; Dario, P. Wireless implantable electronic platform for chronic fluorescent-based biosensors. IEEE Trans. Biomed. Eng. 2011, 58, 1846–1854.2138566610.1109/TBME.2011.2123098 Search in Google Scholar
Vallejos, S.; Munoz, A.; Ibeas, S.; Serna, F.; Garcia, F. C.; Garcia, J. M. Solid sensory polymer substrates for the quantification of iron in blood, wine and water by a scalable RGB technique. J. Mater. Chem. A. 2013, 1, 15435–15441.10.1039/c3ta12703f Search in Google Scholar
Walczak, R.; Kruger, J.; Moynihan, S. A miniaturised image based fluorescence detection system for point-of-care-testing of cocaine abuse. Meas. Sci. Technol. 2015, 26, 15. Search in Google Scholar
Wang, S. Q.; Zhao, X. H.; Khimji, I.; Akbas, R.; Qiu, W. L.; Edwards, D.; Cramer, D. W.; Ye, B.; Demirci, U. Integration of cell phone imaging with microchip ELISA to detect ovarian cancer HE4 biomarker in urine at the point-of-care. Lab Chip2011, 11, 3411–3418.10.1039/c1lc20479c21881677 Search in Google Scholar
Wang, Y.; Li, Y. Y.; Bao, X.; Han, J.; Xia, J. C.; Tian, X. Y.; Ni, L. A smartphone-based colorimetric reader coupled with a remote server for rapid on-site catechols analysis. Talanta2016a, 160, 194–204.10.1016/j.talanta.2016.07.012 Search in Google Scholar
Wang, Y.; Liu, X. H.; Chen, P.; Tran, N. T.; Zhang, J. L.; Chia, W. S.; Boujday, S.; Liedberg, B. Smartphone spectrometer for colorimetric biosensing. Analyst2016b, 141, 3233–3238.10.1039/C5AN02508G Search in Google Scholar
Wang, L. J.; Chang, Y. C.; Sun, R. R.; Li, L. A multichannel smartphone optical biosensor for high-throughput point-of-care diagnostics. Biosens. Bioelectron. 2017, 87, 686–692.2763168310.1016/j.bios.2016.09.021 Search in Google Scholar
Wei, Q.; Qi, H.; Luo, W.; Tseng, D.; Ki, S. J.; Wan, Z.; Göröcs, Z.; Bentolila, L. A.; Wu, T.-T.; Sun, R.; Ozcan, A. Fluorescent imaging of single nanoparticles and viruses on a smart phone. Acs Nano. 2013, 7, 9147–9155.2401606510.1021/nn4037706 Search in Google Scholar
Wei, Q.; Nagi, R.; Sadeghi, K.; Feng, S.; Yan, E.; Ki, S. J.; Caire, R.; Tseng, D.; Ozcan, A. Detection and spatial mapping of mercury contamination in water samples using a smart-phone. Acs Nano. 2014, 8, 1121–1129.10.1021/nn406571t24437470 Search in Google Scholar
Xu, W.; Lu, S.; Chen, Y.; Zhao, T.; Jiang, Y.; Wang, Y.; Chen, X. Simultaneous color sensing of O2 and pH using a smartphone. Sensors Actuators B Chem. 2015, 220, 326–330.10.1016/j.snb.2015.05.088 Search in Google Scholar
Yazawa, Y.; Oonishi, T.; Watanabe, K.; Shiratori, A.; Funaoka, S.; Fukushima, M. System-on-fluidics immunoassay device integrating wireless radio-frequency-identification sensor chips. J. Biosci. Bioeng. 2014, 118, 344–349.2473565210.1016/j.jbiosc.2014.02.010 Search in Google Scholar
Zangheri, M.; Cevenini, L.; Anfossi, L.; Baggiani, C.; Simoni, P.; Di Nardo, F.; Roda, A. A simple and compact smartphone accessory for quantitative chemiluminescence-based lateral flow immunoassay for salivary cortisol detection. Biosens. Bioelectron. 2015, 64, 63–68.10.1016/j.bios.2014.08.04825194797 Search in Google Scholar
©2018 Walter de Gruyter GmbH, Berlin/Boston
This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.