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Licensed Unlicensed Requires Authentication Published by De Gruyter November 8, 2013

Exposure to leachate from municipal battery recycling site: implication as key inhibitor of steroidogenic enzymes and risk factor of prostate damage in rats

  • Jacob K. Akintunde and G. Oboh EMAIL logo

Abstract

Few or no studies have measured the effect of short- and long-term exposure to industrial leachate. Mature male Wistar strain albino rats (175–220 g) underwent sub-chronic exposure to leachate from the Elewi Odo municipal battery recycling site (EOMABRL) via oral administration for a period of 60 days at different doses (20%, 40%, 60%, 80%, and 100%) per kilogram of body weight to evaluate the toxic effects of the leachate on male reproductive function using steroidogenic enzymes and biomarkers of prostate damage. Control groups were treated equally but were given distilled water instead of the leachate. After the treatment periods, results showed that the treatment induced systemic toxicity at the doses tested by causing a significant (p<0.05) loss in absolute body weight and decline in growth rate. There was a marked significant decrease (p<0.05) in testicular activities of Δ5-3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase. Conversely, the activity of prostatic acid phosphatase, a key marker enzyme for prostrate damage was significantly (p<0.05) elevated in the treated rats. Similarly, the administration of EOMABRL significantly (p<0.05) exacerbated the activity of total acid phosphatase with concomitant increase in the activity of prostatic alkaline phosphatase. These findings conclude that exposure to leachate from a battery recycling site induces sub-chronic testicular toxicity by inhibiting key steroidogenic enzymes and activating key markers linked with prostate damage/cancer in rats.


Corresponding author: G. Oboh, Functional Foods, Nutraceuticals and Phytomedicine Research Laboratory, Department of Biochemistry, Federal University of Technology, PMB 704, Akure 340001, Nigeria, Fax: +234-806-4156056, E-mail:

References

1. Mathur PP, D’Cruz SC. The effect of environmental contaminants on testicular function. Asian J Androl 2011;13:585–91.10.1038/aja.2011.40Search in Google Scholar

2. Centers for Disease Control and Prevention. Fourth report on human exposure to environmental chemicals. Atlanta, GA: US Department of Health and Human Services, Centers for Disease Control and Prevention, 2009. Available at: http://www.cdc.gov/exposurereport/. Accessed April 15, 2009.Search in Google Scholar

3. California Environmental Protection Agency. Chemicals known to the state to cause cancer or reproductive toxicity list as of June 22, 2012. Proposition 65: The Safe Drinking Water and Toxic Enforcement Act of 1986. Sacramento, CA: California Environmental Protection Agency. Available at: http://oehha.ca.gov/prop65/prop65_list/files/P65single062212.pdf. Accessed 2012 Jun 22.Search in Google Scholar

4. Farombi EO, Akintunde JK, Nsute N, Adedara IA, Arojojoye O. Municipal landfill leachate induces hepatotoxicity and oxidative stress in rats. J Toxicol Indust Health 2011;28:532–41.10.1177/0748233711416947Search in Google Scholar

5. Akintunde JK, Oboh G. Municipal auto-battery recycling-site leachate activates key enzymes linked to non-insulin dependent diabetes mellitus (NIDDM) and hypertension. J Diabetes Metab 2013;4:235.Search in Google Scholar

6. Azeez LO. Rural water supply in the basement complex of western states, Nigeria. Bull Int Assoc Hydrol Sci 1972;17: 97–110.10.1080/02626667209493806Search in Google Scholar

7. Lagos State Waste Management Authority. An official report on tonnage of different types of waste collected and disposed by LAWMA between 1988–1998. Lagos, Nigeria: LAWMA, 2000.Search in Google Scholar

8. Bagchi A. Design of landfills and integrated solid waste management: landfill design, 3rd ed. New York: Wiley & Sons, 2004.Search in Google Scholar

9. Monroe M. Landfill leachate treatment: VSEP offers a revolutionary solution. Emeryville, CA: VSEP, 2001.Search in Google Scholar

10. Speidel DH, Ruedisili LC, Agnew AF. Perspectives on water, uses and abuses. New York: Oxford University Press, 1988.Search in Google Scholar

11. Forstner U. Contaminated sediments: lecture notes in earth sciences. Berlin: Springer, 1989.Search in Google Scholar

12. Dolk H. Risk of congenital anomalies near hazardous waste landfill sites in Europe: the EUROHAZCON study. Lancet 1999;352:423–7.10.1016/S0140-6736(98)01352-XSearch in Google Scholar

13. Radi LM, Kuntz DJ, Padmanabham G, Berg IE, Chatvrved AK. Toxicological evaluation of the leachate from a closed urban landfill. Bull Environ Contam Toxicol 1987;38:337–44.10.1007/BF01606684Search in Google Scholar

14. Bjorling-Poulsen M, Andersen HR, Grandjean P. Potential developmental neurotoxicity of pesticides used in Europe. Environ Health 2008;7:50–71.10.1186/1476-069X-7-50Search in Google Scholar

15. Bruckner JV. Differences in sensitivity of children and adults to chemical toxicity: the NAS panel report. Regul Toxicol Pharmacol 2000;31:280–5.10.1006/rtph.2000.1393Search in Google Scholar

16. Grandjean P, Landrigan PJ. Developmental neurotoxicity of industrial chemicals. Lancet 2006;368:2167–78.10.1016/S0140-6736(06)69665-7Search in Google Scholar

17. Mendola P, Selevan SG, Gutter S, Rice D. Environmental factors associated with a spectrum of neurodevelopmental deficits. Ment Retard Dev Disabil Res Rev 2002;8:188–97.10.1002/mrdd.10033Search in Google Scholar PubMed

18. Rice DC. Assessing the effects of environmental toxicant exposure in developmental epidemiological studies: issues for risk assessment. Neurotoxicology 2005;26:483–9.10.1016/j.neuro.2004.12.009Search in Google Scholar PubMed

19. Wigle DT, Arbuckle TE, Turner MC, Bérubé A, Yang Q, et al. Epidemiologic evidence of relationships between reproductive and child health outcomes and environmental chemical contaminants. J Toxicol Environ Health B 2008;11:373–517.10.1080/10937400801921320Search in Google Scholar

20. Woodruff T. Proceedings of the Summit on Environmental Challenges to Reproductive Health and Fertility: executive summary. Fertil Steril 2008;89:e1–20.10.1016/j.fertnstert.2008.01.065Search in Google Scholar

21. Vandenberg LN, Colborn T, Hayes TB, Heindel JJ, Jacobs DR Jr, et al. Hormones and endocrine-disrupting chemicals: low-dose effects and nonmonotonic dose responses. Endocr Rev 2012;33:378–455.10.1210/er.2011-1050Search in Google Scholar

22. Houk VS. The genotoxicity of industrial wastes and effluents. Mutat Res 1992;277:91–138.10.1016/0165-1110(92)90001-PSearch in Google Scholar

23. Siddique HR, Gupta SC, Dhawan A, Murthy RC, Saxena DK, et al. Genotoxicity of industrial solid waste leachates in Drosophila melanogaster environ. Mol Mutagen 2005;46:189–97.10.1002/em.20149Search in Google Scholar

24. American Society for Testing Material. Standard method for shake extraction of solid waste with water. ASTM D3987-85. West Conshohocken, PA: ASTM, 1992.Search in Google Scholar

25. Ferrari B, Radetski CM, Veber AM, Ferard JF. Ecolotoxicological assessment of solid wastes: a combined liquid- and solid-phase testing approach using a battery of bioassays and biomarkers. Environ Toxicol Chem 1999;18:1195–202.Search in Google Scholar

26. Association of Official Analytical Chemists. Official method of analysis, 15th ed. Washington, DC: AOAC, 2005.Search in Google Scholar

27. Longe EO, Balogun MR. Groundwater quality assessment near a municipal landfill, Lagos, Nigeria. Res J Appl Sci Eng Technol 2010;2:39–44.Search in Google Scholar

28. Longe EO, Enekwechi LO. Investigation on potential groundwater impacts and influence of local hydrogeology on natural attenuation of leachate at a municipal landfill. Int J Environ Sci Technol 2007;4:133–40.10.1007/BF03325971Search in Google Scholar

29. Nduka JKC, Orisakwe OE, Nwangunna CK. Heavy metals other than lead in flaked paints from buildings in Eastern Nigeria. Toxicol Indust Health 2007;23:525–8.10.1177/0748233708089038Search in Google Scholar

30. Halliwell B, Gutteridge JMC, Arouma OI. The deoxyribose method: simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 1997;165:215–9.10.1016/0003-2697(87)90222-3Search in Google Scholar

31. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957;28:56–63.10.1093/ajcp/28.1.56Search in Google Scholar

32. Englehardt A. Measurement of alkaline phosphatase. Aerztl Labor 1970;16:42.Search in Google Scholar

33. Szasz G, Bergmeyer HU, editors. Method of enzymatic analysis. Weinheim: Verlag Chemie, 1974.Search in Google Scholar

34. Seiler D, Nagel D, Tritschler W, Looser S. Saure phosphatase in serum (substrate: alpha-Naphthylphosphat) Referenczwerte und Dianostisce Aussage. J Clin Chem Biochem 1983;21: 519–25.Search in Google Scholar

35. Talalay P. Hydroxysteroid dehydrogenase. In: Colowick SP, Kalplan NO, editors. Methods in enzymology, vol 5: New York: Elsevier, 1962:512–26.Search in Google Scholar

36. Jarabak J, Adams JA, Williams-Ashaman HG, Talalay P. Purification of 17β-hydroxyl steroid dehydrogenase function. J Biol Chem 1962;237:345–57.10.1016/S0021-9258(18)93926-8Search in Google Scholar

37. Zar JH. Biostatistical analysis. USA: Prentice-Hall Inc., 1984.Search in Google Scholar

38. Akintunde JK, Oboh G. In vitro oxidative damage induced in livers, hearts and kidneys of rats treated with leachate from battery recycling site: evidence for environmental contamination and tissue damage. J Clin Exp Pathol 2012;2:129.Search in Google Scholar

39. Nolan K. Copper toxicity syndrome. J Orthomol Psychiatry 2003;12:270–82.Search in Google Scholar

40. Ogwuegbu MO, Ijioma MA. Effects of certain heavy metals on the population due to mineral exploitation. In: International Conference on Scientific and Environmental Issues in the Population, Environmental and Sustainable Development in Nigeria. Ekiti State, Nigerian: University of Ado, 2003:8–10.Search in Google Scholar

41. Miller MJ, Sadowska-Krowicka H, Chotinaruemol S, Kakkis JL, Clark DA. Amelioration of chronic ileitis by nitric oxide synthase inhibition. J Pharmacol Exp Ther 1993;264:11–6.Search in Google Scholar

42. Abdel-Wahhab MA, Ahmed HH. Protective effect of Korean panax ginseng against chromium VI toxicity and free radical generation in rats. J Ginseng Res 2004;28:11–7.10.5142/JGR.2004.28.1.011Search in Google Scholar

43. Pant N, Srivastava SP. Testicular and spermatotoxic effect of quinaphos in rats. J Appl Toxicol 2003;23:271–4.10.1002/jat.919Search in Google Scholar

44. Farombi EO, Adelowo OA, Ajimoko YR. Biomarkers of oxidative stress and heavy metal levels as indicator of environmental pollution in Africal cat fish (Clarias gariepinus) from Nigerian Ogun river. Int J Environ Res Publ Health 2007;4:158–65.10.3390/ijerph2007040011Search in Google Scholar

45. Grupta RS, Sharma R, Sharma A, Bhatnager AK, Dobhal MP, et al. Effects of Alstonia scholaris bark extract on testicular function of Wistar rats. Asian J Androl 2002;4:175–8.Search in Google Scholar

46. Mouraviev V, Mayes JM, Polascik TJ. Pathologic basis of focal therapy for early-stage prostate cancer. Nat Rev Urol 2009;6:205–15.10.1038/nrurol.2009.29Search in Google Scholar

47. Noguchi M, Stamey TA, McNeal JE, Nolley R. Prognostic factors for multifocal prostate cancer in radical prostatectomy specimens: lack of significance of secondary cancers. J Urol 2011;170:459–63.10.1097/01.ju.0000070928.49986.04Search in Google Scholar

48. Taira A, Merrick G, Wallner K, Dattoli M. Reviving the acid phosphatase test for prostate cancer. Oncology 2007;21: 1003–10.Search in Google Scholar

49. Varley H, Gowenlock AH, Bell H. Enzymes. In: William H, editor. Practical clinical biochemistry. London: William Heinemann Medical Books, 1980:813–8.Search in Google Scholar

50. Ekwere PD, Egbe SN. The changing pattern of prostate cancer in Nigerians: current status in the southeastern states. J Natl Med Assoc 2002;94:619–27.Search in Google Scholar

51. Gadre SG, Kale KU, Hedge R, Raste AS. Comparative study of alkaline phosphatase and prostate specific antigen in prostate cancer. Indian J Med Sci 2000;54:136–9.Search in Google Scholar

52. Wajsman Z, Chu TM, Bross D, Saroff J, Murphy GP, et al. Clinical significance of serum alkaline phosphatase isoenzymes levels in advanced prostate carcinoma. J Urol 1978;119;244–6.10.1016/S0022-5347(17)57446-7Search in Google Scholar

53. Lindner U, Lawrentschuk N, Trachtenberg J. Focal laser ablation for localized prostate cancer. J Endourol 2010;24:791–7.10.1089/end.2009.0440Search in Google Scholar

54. Colin P, Mordon S, Nevoux P, Feras Marqa M, Quzzane A, et al. Focal laser ablation of prostate cancer: definition, needs, and future. J Adv Urol 2012;2012:1–10.10.1155/2012/589160Search in Google Scholar

55. Jana K, Jana S, Samanta PK. Effects of chronic exposure to sodium arsenate on hypothalamus-pituitary-testicular activities in adult rats: possible anaestrogenic mode of action. Reprod Biol Endocrinol 2006;4:9.10.1186/1477-7827-4-9Search in Google Scholar

56. Strauss JF. The synthesis and metabolism of steroid hormones. In: Strauss JF, Barbieri RL, editors. Yen and Jaffe’s reproductive endocrinology, vol 5. Saunders, PA: Elsevier, 2004:125–54.Search in Google Scholar

57. Ghosh D, Debnath JM. Evaluation of leaf extract of Stephania hernandifolia on testicular gametogenesis and androgenesis in albino rats: a dose-dependent respose study. Contraception 2002;65:379–84.10.1016/S0010-7824(02)00282-2Search in Google Scholar

58. Shimomura K, Shimada M, Hagiwara M, Harada S, Kato M, et al. Insight into testicular damage induced by ethinylestradoil in rats. Reprod Toxicol 2005;20:157–63.10.1016/j.reprotox.2004.12.013Search in Google Scholar PubMed

59. Odell WD, Swerdloff RS, Bain J, Wallesen F, Grover PK. The effect of sexual maturation testicular response to LH stimulation of testosterone secretion in the intact rat. Endocrinology 1963;72:452–64.Search in Google Scholar

60. Ghosh D, Chattopadhyay S, Debnath J. Effect of sodium arsenite on adrenocortical activity in immature female rats: evidence of dose dependent response. J Environ Sci 1999;11:419–22.Search in Google Scholar

61. Christian JJ. Actions of ACTH in intact and corticoid-maintained adrenalectomized female mice with emphasis on the reproductive tract. Endocrinology 1964;75:655–7.10.1210/endo-75-5-653Search in Google Scholar PubMed

62. Luton J, Thiebott P, Valcke J, Mahendean JA, Bricaire H. Reversible gonadotropin deficiency in male Cushing disease. J Clin Endocrinol Metab 1977;45:488–93.10.1210/jcem-45-3-488Search in Google Scholar PubMed

63. Kamel FA, Kubajak CL. Modulation of gonadotropin secretion by corticosterone interaction with gonadal steroids and mechanism of action. Endocrinology 1987;121, 561–5.10.1210/endo-121-2-561Search in Google Scholar PubMed

64. Ogle TF. Modification of serum leuteinizing hormone and prolactin concentration by corticotropin and adrenalectomy in ovariectomized rats. Endocrinology 1977;101:494–7.10.1210/endo-101-2-494Search in Google Scholar PubMed

65. Ringstrom SJ, Schwartz NB. Cortisol suppresses the LH release and ovulation in cyclic rat. Endocrinology 1985;116:472–5.Search in Google Scholar

66. Gunnarsson D, Svensson M, Setstam G, Nordberg G. Pronounced induction of testicular PGF2_ and suppression of testosterone by cadmium-prevention by zinc. Toxicology 2004;200:49–58.10.1016/j.tox.2004.03.003Search in Google Scholar PubMed

Received: 2013-8-1
Accepted: 2013-10-10
Published Online: 2013-11-08
Published in Print: 2013-12-01

©2013 by Walter de Gruyter Berlin Boston

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