Skip to content
Licensed Unlicensed Requires Authentication Published by Oldenbourg Wissenschaftsverlag March 28, 2017

Spatial monitoring of the liquid phase with multiparameter sensors in industrial-scale fermenters

Ortsaufgelöste Prozessbeobachtung mit Mehrparametersensorik in der Flüssigphase industrieller Fermenter
Ute Enseleit

Ute Enseleit is research scientist at the Kurt-Schwabe-Institut für Mess- und Sensortechnik e. V. Meinsberg. Focus areas: Environmental- and trace analytics (HPLC, GC, IC, AAS), Development of elctrochemical sensors.

Kurt Schwabe Research Institute, Kurt-Schwabe Straße 4, 04736 Waldheim, Germany

EMAIL logo
, Anika Bockisch

Anika Bockisch is research scientist at the chair of bioprocess engineering at TU Berlin. Focus areas: Fermentation technology, brewing process, process analytical technologies (PAT).

TU Berlin, Department of Biotechnology, Chair of Bioprocess Engineering, Ackerstrasse 76 ACK24, 13355 Berlin, Germany

, Sandra Sachse

Sandra Sachse ist research scientist at the Kurt-Schwabe-Institut für Mess- und Sensortechnik e. V. Meinsberg. Focus area: bioprocess engineering.

Kurt Schwabe Research Institute, Kurt-Schwabe Straße 4, 04736 Waldheim, Germany

, Erich Kielhorn

Erich Kielhorn is currently working as project manager at the Forschungszentrum Jülich GmbH – Projektträger Jülich, Focus areas: Energy and electromobility

Projektträger Jülich, Zimmerstraße 26, 10969 Berlin, Germany

, Peter Neubauer

Peter Neubauer is head of the chair of bioprocess engineering at TU Berlin. Focus area is the optimization of all kinds of microbial bioprocesses under consideration of physiological aspects.

TU Berlin, Department of Biotechnology, Chair of Bioprocess Engineering, Ackerstrasse 76 ACK24, 13355 Berlin, Germany

, Stefan Junne

Stefan Junne is group leader at the chair of bioprocess engineering at TU Berlin. Focus areas: Scale up and down of bioprocesses, process analytical technologies (PAT) and single-use bioreactor development.

TU Berlin, Department of Biotechnology, Chair of Bioprocess Engineering, Ackerstrasse 76 ACK24, 13355 Berlin, Germany

, Martin Bertau

Martin Bertau is head of the Institute of Technical Chemistry. Focus areas: white biotechnology and sustainable production processes for energy resources.

TU Bergakademie Freiberg, Faculty of Physics and Chemistry, Institute of Technical Chemistry, Leipziger Straße 29, 09596 Freiberg, Germany

and Winfried Vonau

Winfried Vonau is Vice-Director at the Kurt-Schwabe-Institut für Mess- und Sensortechnik e. V. Meinsberg. Focus areas: Development of electrochemical sensors and sensor materials.

Kurt Schwabe Research Institute, Kurt-Schwabe Straße 4, 04736 Waldheim, Germany

From the journal tm - Technisches Messen

Abstract

This work describes the development and application of different lance-based monitoring systems coupled to an integrated multiparameter sensor probe for the spatial investigation of the liquid phase in biotechnological processes. Miniaturized electrochemical sensors are integrated into a housing, which is fixed at the head of a lance, both made of stainless steel. It can be moved inside a reactor in horizontal direction by the lance itself and in vertical direction by a flexible rope, which is connected to the sensor probe. Inserting the sensor unit directly into the liquid phase of a fermenter, e.g. in laminar processes like biogas and beer production, coupled with multiposition measurements allows to obtain space and time resolved data. The monitored parameters are the pH value, the redox potential, the temperature, and the concentration of dissolved carbon dioxide. Wireless data transfer is realized by means of radio technology with the virtual control center RAMOC (Radio Aided Monitoring) of the company innotas GmbH. Results of the real-time determination in the liquid phase in an industrial, continuously operated biogas plant during several weeks are presented as a case study.

Zusammenfassung

In dem vorliegenden Beitrag wird die Entwicklung und Erprobung von verschiedenen Lanzen-basierten Systemen mit integrierter Multiparameter-Sensorsonde zur ortsaufgelösten Untersuchung der Flüssigphase in biotechnologischen Prozessen vorgestellt. In den aus Edelstahl gefertigten Lanzenkopf ist eine Edelstahl-Sonde integriert, die mit prozesstauglichen, miniaturisierten, elektrochemischen Sensoren bestückt ist. Die Sonde kann im Tank in horizontaler Richtung mittels der Lanze sowie in vertikaler Richtung mit Hilfe eines flexiblen Seiles, das an der Sonde angebracht ist, bewegt werden. Das Einbringen der Lanzenkörper direkt in die Flüssigphase eines Fermenters, z. B. in einem laminaren Prozess wie Biogas- und Bierproduktion, zusammen mit dem Multipositionsansatz ermöglicht die Aufnahme von örtlich und zeitlich aufgelösten Daten. Die erfassten Messgrößen sind der pH-Wert, das Redoxpotential, die Temperatur und die Konzentration von gelöstem Kohlendioxid. Die kabellose Datenübertragung erfolgt hierbei mittels Funktechnik über die Leitstelle RAMOC. Als Fallstudie werden die Ergebnisse der Echtzeit-Bestimmungen in der Flüssigphase in einer industriellen, kontinuierlich betriebenen Biogasanlage über mehrere Wochen vorgestellt.

About the authors

Ute Enseleit

Ute Enseleit is research scientist at the Kurt-Schwabe-Institut für Mess- und Sensortechnik e. V. Meinsberg. Focus areas: Environmental- and trace analytics (HPLC, GC, IC, AAS), Development of elctrochemical sensors.

Kurt Schwabe Research Institute, Kurt-Schwabe Straße 4, 04736 Waldheim, Germany

Anika Bockisch

Anika Bockisch is research scientist at the chair of bioprocess engineering at TU Berlin. Focus areas: Fermentation technology, brewing process, process analytical technologies (PAT).

TU Berlin, Department of Biotechnology, Chair of Bioprocess Engineering, Ackerstrasse 76 ACK24, 13355 Berlin, Germany

Sandra Sachse

Sandra Sachse ist research scientist at the Kurt-Schwabe-Institut für Mess- und Sensortechnik e. V. Meinsberg. Focus area: bioprocess engineering.

Kurt Schwabe Research Institute, Kurt-Schwabe Straße 4, 04736 Waldheim, Germany

Erich Kielhorn

Erich Kielhorn is currently working as project manager at the Forschungszentrum Jülich GmbH – Projektträger Jülich, Focus areas: Energy and electromobility

Projektträger Jülich, Zimmerstraße 26, 10969 Berlin, Germany

Peter Neubauer

Peter Neubauer is head of the chair of bioprocess engineering at TU Berlin. Focus area is the optimization of all kinds of microbial bioprocesses under consideration of physiological aspects.

TU Berlin, Department of Biotechnology, Chair of Bioprocess Engineering, Ackerstrasse 76 ACK24, 13355 Berlin, Germany

Stefan Junne

Stefan Junne is group leader at the chair of bioprocess engineering at TU Berlin. Focus areas: Scale up and down of bioprocesses, process analytical technologies (PAT) and single-use bioreactor development.

TU Berlin, Department of Biotechnology, Chair of Bioprocess Engineering, Ackerstrasse 76 ACK24, 13355 Berlin, Germany

Martin Bertau

Martin Bertau is head of the Institute of Technical Chemistry. Focus areas: white biotechnology and sustainable production processes for energy resources.

TU Bergakademie Freiberg, Faculty of Physics and Chemistry, Institute of Technical Chemistry, Leipziger Straße 29, 09596 Freiberg, Germany

Winfried Vonau

Winfried Vonau is Vice-Director at the Kurt-Schwabe-Institut für Mess- und Sensortechnik e. V. Meinsberg. Focus areas: Development of electrochemical sensors and sensor materials.

Kurt Schwabe Research Institute, Kurt-Schwabe Straße 4, 04736 Waldheim, Germany

Acknowledgement

This work was funded by the German Federal Ministry of Education and Research (BMBF) within the framework program “BioProFi”, under coordination of the project management Jülich, grant no. 03SF0455A and C.

Received: 2016-10-7
Revised: 2016-11-16
Accepted: 2017-2-15
Published Online: 2017-3-28
Published in Print: 2017-10-26

©2017 Walter de Gruyter Berlin/Boston

Downloaded on 7.12.2022 from frontend.live.degruyter.dgbricks.com/document/doi/10.1515/teme-2016-0084/html
Scroll Up Arrow