Abstract
In this work, we report the Nocardia species were initially screened and then tested for their ability to produce biosurfactant. The biosurfactant production from the Nocardia species was determined by qualitative standard methods. The selected bacterial strain had better oil spreading and emulsifying activity and surface tension reduction. The biosurfactant producer strain was identified by 16S rRNA gene sequencing method. Using Fourier transform infrared spectroscopy and comparative analysis, the biosurfactant structure could be detected. Oil spreading tests and blue agar plate tests confirmed the presence of biosurfactant and extracellular anionic glycolipids. The E24% assay using olive oil showed strong emulsifying characteristic of extracted biosurfactant with a 100%. The stability of the biosurfactant produced in different conditions was significant. Nocardia coubleae was identified by biochemical methods and molecular methods.
Abstract
In dieser Arbeit haben wir zunächst Nocardia-Arten gescreent und danach ihre Fähigkeit zur Produktion von Biotensiden untersucht. Die Produktion von Biotensiden aus den Nocardia-Spezies wurde mit qualitativen Standardmethoden bestimmt. Der ausgewählte Bakterienstamm hatte eine bessere Spreitung auf Öl, Emulgieraktivität und Verringerung der Oberflächenspannung. Der Biotensid erzeugende Stamm wurde mittels der 16S rRNA-Gensequenzierungsmethode identifiziert. Mittels der Fourier-Transformations-Infrarotspektroskopie und Vergleichsanalyse konnte die Biotensid-Struktur nachgewiesen werden. Mit Ölspreitungtests und Blauagarplattentests wurde die Anwesenheit von Biotensid und extrazellulären anionischen Glykolipiden bestätigt. Der E24%-Test mit Olivenöl zeigte eine starke Emulgiereigenschaft des extrahierten Biotensids von 100%. Die Stabilität des unter verschiedenen Bedingungen hergestellten Biotensids war signifikant. Nocardia coubleae wurde durch biochemische Methoden und molekulare Methoden identifiziert.
About the authors
Ali Javadi: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mohamad Reza Pourmand: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Javad Hamedi: Microbial Biotechnology Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
Fatemeh Gharebaghi: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Zohre Baseri: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Hossein Ali Rahdar: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abbas Morovvati: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Razieh Mohammadzadeh: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Seyyed Saeed Eshraghi: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Conflict of Interest
The authors declare that they have no competing interests
Ethical issues
This research was approved by the ethics committee of Tehran University of Medical Sciences, Tehran, Iran
(Ethic of Number: IR.TUMS.SPH.REC.1396.3205).
Authors’ contributions
All authors were involved in study design, data collection, and article approval.
Acknowledgements
This study was part of a PhD thesis supported by Tehran University of Medical Science (Grant No: 34468270396).
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