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Accessible Unlicensed Requires Authentication Published by De Gruyter November 30, 2021

Micellar-Enhanced Spectrofluorimetric Method for Quantification of Diclofenac Potassium in Pure Form, in Pharmaceutical Preparations and Human Plasma

Mit Mizellen unterstützte spektrofluorimetrische Methode zur Quantifizierung von Diclofenac-Kaliumsalz in reiner Form, in pharmazeutischen Zubereitungen und in menschlichem Plasma
Muhammad Naeem Khan, Irum, Saba Gul, Muslima and Muhammad Mursaleen


A rapid, simple and economical spectrofluorimetric method for the determination of diclofenac potassium in pure form, in pharmaceutical preparations and in human plasma has been developed. The method is based on the enhancement of the fluorescence signal of diclofenac potassium by the addition of sodium dodecyl sulphate in McIvaine buffer with a pH of 5. Different experimental conditions such as buffer type, pH, type and concentration of surfactants were investigated. The fluorescence intensity of the solution was recorded at 361 nm after excitation at 243 nm. The method shows linearity in the concentration range of 0.2 μg mL–1–10 μg mL–1 with a good correlation coefficient of 0.997. The relative standard deviation value was 3.62 (n = 7). The limit of detection and limit of quantification were calculated to be 2.84 × 10–3 μg mL–1 and 9.47 × 10–3 μg mL-1, respectively. The effect of excipients and co-administrated drugs was investigated and no interference was observed. The method was successfully applied for the determination of diclofenac potassium in pure form, in pharmaceutical products and in human plasma. The percentage recoveries obtained ranged from 100.25% to 102.16% for pure form and 97.50% to 102.00% for pharmaceutical products and from 98.50% to 101.67% for human plasma.


Es wurde ein schnelles, einfaches und wirtschaftliches spektrofluorimetrisches Verfahren zur Bestimmung von Diclofenac-Kaliumalz in reiner Form, in pharmazeutischen Produkten und in menschlichem Plasma entwickelt. Die Methode basiert auf der Verstärkung des Fluoreszenzsignals von Diclofenac-Kaliumsalz durch die Zugabe von Natriumdodecylsulfat in McIvaine-Puffer mit einem pH-Wert von 5. Es wurden verschiedene Versuchsbedingungen wie Pufferart, pH-Wert, Art und Konzentration der Tenside untersucht. Die Fluoreszenzintensität der Lösung wurde bei 361 nm und nach Anregung bei 243 nm aufgezeichnet. Die Methode zeigt lineares Verhalten im Konzentrationsbereich von 0,2 μg mL–1–10 μg mL–1 mit einem guten Korrelationskoeffizienten von 0,997. Die relative Standardabweichung betrug 3,62 (n = 7). Die berechnete Nachweis- und Bestimmungsgrenze betrug 2,84 × 10–3 μg mL–1 bzw. 9,47 × 10–3 μg mL–1. Die Einfluss von Hilfsstoffen und gleichzeitig verabreichten Arzneimitteln wurde untersucht und es wurden keine Interferenzen festgestellt. Die Methode wurde erfolgreich für die Bestimmung von Diclofenac-Kalium in reiner Form, in pharmazeutischen Produkten und in menschlichem Plasma eingesetzt. Die erzielten prozentualen Wiederfindungen lagen zwischen 100,25% und 102,16% für die reine Form und zwischen 97,50% und 102,00% für pharmazeutische Produkte sowie zwischen 98,50% und 101,67% für Humanplasma.

Muhammad Naeem Khan Department of Chemistry Bacha Khan University 24420 Charsadda Pakistan


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Received: 2020-07-05
Accepted: 2021-06-16
Published Online: 2021-11-30

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