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Screening of Biosurfactant Producing Bacteria Isolated from Hydrocarbon Contaminated Site and Their Potential in Biosorption of Pb(II) and Oil Biodegradation

Screening von Biosurfactant-produzierenden Bakterien, die von Kohlenwasserstoff-kontaminierten Standorten isoliert wurden, und ihr Potenzial für die Biosorption von Pb(II) und den Abbau von Öl
Swati Rastogi, Sheel Ratna and Rajesh Kumar


In the present study, three potentially Pb(II)-resistant and biosurfactant-producing bacterial strains were isolated from a total of 23 strains using various screening methods, investigated for their biosorption of Pb(II) and used for the biodegradation of used motor oil. The results show that strain E1 (Bacillus haynesii) has significantly high efficiency in biodegradation of used motor oil, up to 82 % in the first three days. Maximum Pb(II) biosorption capacities of 238.09 mg/g and 99.01 mg/g were determined for strains E1 and F5 (Pseudomonas aeruginosa), respectively. The biosorption process was found to be in good agreement with the Langmuir isotherm for both E1 (R2 = 0.9614) and F5 (R2 = 0.9646), suggesting monolayer biosorption. The four common screening methods, namely the haemolytic assay, the determination of surface tension, the emulsifying activity and the foam test, were also correlated with the Pearson correlation method.


In der vorliegenden Studie wurden drei potenziell Pb(II)-resistente und Biotensid-produzierende Bakterienstämme aus insgesamt 23 Stämmen mit Hilfe verschiedener Screening-Methoden isoliert, auf ihre Biosorption von Pb(II) untersucht und für den biologischen Abbau von gebrauchtem Motoröl eingesetzt. Die Ergebnisse zeigen, dass der Stamm E1 (Bacillus haynesii) eine signifikant hohe Effizienz beim biologischen Abbau von gebrauchtem Motoröl aufweist, nämlich bis zu 82 % in den ersten drei Tagen. Für die Stämme E1 und F5 (Pseudomonas aeruginosa) wurden maximale Pb(II)-Biosorptionskapazitäten von 238,09 mg/g bzw. 99,01 mg/g ermittelt. Es wurde festgestellt, dass der Biosorptions-prozess sowohl für E1 (R2 = 0,9614) als auch für F5 (R2 = 0,9646) gut mit der Langmuir-Isotherme übereinstimmt, was auf eine Monolayerbiosorption schließen lässt. Die vier gängigen Screening-Methoden, nämlich der hämolytische Assay, die Bestimmung der Oberflächenspannung, die Emulgieraktivität und der Schaumtest, wurden ebenfalls mit der Pearson-Korrelationsmethode korreliert.

Ms. Swati Rastogi Research Scholar Rhizosphere Biology Laboratory Department of Environmental Microbiology Babasaheb Bhimrao Ambedkar (A Central) University Vidya Vihar Raebareli Road Lucknow-226025 India Tel.: 07607487423

Funding statement: One of the authors (Sheel Ratna) is highly thankful to research grant 3721/(SC) (NET-NOV 2017) University Grant Commission, New Delhi, India.


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Received: 2020-12-22
Accepted: 2021-06-30
Published Online: 2021-11-30

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