Accessible Unlicensed Requires Authentication Published by De Gruyter November 30, 2021

A Purified Alkaline and Detergent-Tolerant Lipase from Aspergillus fumigatus with Potential Application in Removal of Mustard Oil Stains from Cotton Fabric

Eine gereinigte, alkali- und waschmitteltolerante Lipase aus Aspergillus fumigatus mit Anwendungspotenzial bei der Entfernung von Senfölflecken aus Baumwollgeweben
Akshita Mehta, Aashna Gupta, Kamal Kumar Bhardwaj and Reena Gupta


In the present study, the lipase from Aspergillus fumigatus was purified which was found to be stable to commercial detergents and oxidising agents. A purification fold of 6.96 and yield of 11.03% were achieved when the enzyme was purified using Octyl Sepharose column chromatography. In presence of various oxidizing agents, the highest activity of lipase was 15.56 U/mg with hydrogen peroxide. Among various surfactants used, the maximum activity exhibited by lipase was with Tween 80. While studying the effect of various detergents, the highest activity of 9.3 U/mg was achieved with "Vanish" detergent. Wash performance was studied with various detergents out of which "Vanish" showed highest oil removal of 79%. Lipase from Aspergillus fumigatus possessed better stability with various surfactants and oxidizing agents. The results of this study have shown that the lipase from Aspergillus fumigatus along with detergent "Vanish" (0.7%) under optimized conditions (5 μg/ml lipase, 40°C wash temperature and 40 min wash duration) improved oil removal from cotton fabric stained with mustard oil by 84%.


In der vorliegenden Studie wurde die Lipase aus Aspergillus fumigatus gereinigt, die sich gegenüber handelsüblichen Waschmitteln und Oxidationsmitteln als stabil erwies. Eine Reinigungsfalte von 6,96 und eine Ausbeute von 11,03% wurden erreicht, als das Enzym mit Octyl-Sepharose-Säulenchromatographie gereinigt wurde. In Anwesenheit verschiedener Oxidationsmittel betrug die höchste Aktivität der Lipase 15,56 U/mg mit Wasserstoffperoxid. Unter den verschiedenen verwendeten Tensiden war die maximale Aktivität der Lipase mit Tween 80 zu verzeichnen. Bei der Untersuchung der Wirkung verschiedener Waschmittel wurde die höchste Aktivität von 9,3 U/mg mit dem Waschmittel Vanish erreicht. Die Waschleistung wurde mit verschiedenen Waschmitteln untersucht, von denen "Vanish" die beste Ölentfernung von 79% zeigte. Lipase aus Aspergillus fumigatus besaß eine bessere Stabilität mit verschiedenen Tensiden und Oxidationsmitteln. Die Ergebnisse dieser Studie haben gezeigt, dass die Lipase aus Aspergillus fumigatus zusammen mit dem Waschmittel ,,Vanish" (0,7%) unter optimierten Bedingungen (5 μg/ml Lipase, 40°C Waschtemperatur und 40 min Waschdauer) die Ölentfernung aus mit Senföl angeschmutzem Baumwollstoff um 84% verbesserte.

Prof. Dr. Reena Gupta Dean, Faculty of Life Sciences Department of Biotechnology Himachal Pradesh University Summerhill Shimla (H.P.) 171 005, INDIA

Funding statement: The financial support from DEST (Department of Environment, Science and Technology), Himachal Pradesh to Ms. Akshita Mehta in the form of Project Fellow is thankfully acknowledged. The Fellowship granted to Mr. Kamal Kumar Bhardwaj in the form of SRF from the Council of Scientific & Industrial Research under Ministry of Human Resource Development, Government of India is thankfully acknowledged.

  1. Author’s Contributions:

    Mrs. Akshita Mehta had performed the experimental work and result analysis.

    Ms. Aashna Gupta assisted in the methodology and as well as in experiments done.

    Mr. Kamal Kumar Bhardwaj helped in the writing of the manuscript.

    Dr. Reena Gupta had designed the experiments and critically revised writing and the organization of the manuscript.

  2. Conflicts of interest:

    The author(s) declare(s) that there is no conflict of interests regarding the publication of this article.


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Received: 2021-06-11
Accepted: 2021-08-11
Published Online: 2021-11-30

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