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The Thai neem A. siamensis (Valeton) is the main source for botanical insecticides in Thailand. Although azadirachtin A (aza A) is present in Thai neem, there are some other related compounds which may contribute to its bioefficiency. Analytical HPLC of Thai neem samples showed a peak closely eluted with azadirachtin. A large-scale cleanup involving two liquid/liquid extraction steps, silica filtration, and finally two successive preparative HPLC separations, resulted in the pure compound, which was identified as 1-tigloyl-3-acetylazadirachtol by 1D -and 2D -1H and 13C NMR spectroscopy.
Published Online: 2014-6-2
Published in Print: 1997-11-1
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