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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Preparation and Characterization of Actin from Liver

  • B. Jaberg

G-actin was prepared from pork liver by gelfiltration on AcA 44 Ultrogel. The purity of the actin was 95-98% and the yield was 45-150 mg from 500 g liver, corresponding to a maximum efficiency of recovery of about 30%.

High viscosities of actin-enriched solutions were reduced by a specific pH-step. In SDS-gel electrophoresis a single band of about ~ 45000 daltons was seen. The G-actin was polymerizable as shown by high shear viscosity measurements. The isoelectric points of the β- and γ-isoactins were 5.6 and 5.7.

Received: 1983-6-28
Revised: 1983-8-13
Published Online: 2014-6-2
Published in Print: 1983-10-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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