The content of ColE 1-related plasmids increased about 4-6-fold after a temperature-shift from 30 to 42 °C (45 °C) if the rom-region of the plasmids was deleted.
The copy number of rom+-plasmids did not change after the temperature shift. All rom¯-plasmids tested in this study showed this plasmid amplification. The Rom-protein is capable of inhibiting plasmid replication by stabilization the initial reversible stage of the association of RNAI with the primer precursor RNAII.
We suggest that the temperature-dependent enhancement of the copy number of rom¯-plasmids is due to a destabilization of this initial phase of the RNAI-preprimer interaction at high temperatures which is suppressed by the Rom-protein in cells with rom+-plasmids.
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