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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Replikation ColE 1-verwandter Plasmide bei erhöhter Wachstumstemperatur in Abhängigkeit von der Rom-Funktion / Replication of ColE 1-Related Plasmids at Increased Growth Temperature in Dependence on the Rom Function

  • Sabine Riethdorf , Andreas Ulrich and Michael Hecker

The content of ColE 1-related plasmids increased about 4-6-fold after a temperature-shift from 30 to 42 °C (45 °C) if the rom-region of the plasmids was deleted.

The copy number of rom+-plasmids did not change after the temperature shift. All rom¯-plasmids tested in this study showed this plasmid amplification. The Rom-protein is capable of inhibiting plasmid replication by stabilization the initial reversible stage of the association of RNAI with the primer precursor RNAII.

We suggest that the temperature-dependent enhancement of the copy number of rom¯-plasmids is due to a destabilization of this initial phase of the RNAI-preprimer interaction at high temperatures which is suppressed by the Rom-protein in cells with rom+-plasmids.

Received: 1991-5-6
Revised: 1991-7-8
Published Online: 2014-6-2
Published in Print: 1991-12-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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