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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Partially N-Deacetylated Chitin Fragments are Strong Elicitors for (+)-Pisatin Induction in Epicotyls of Pea

  • Akio Kobayashi , Kohki Akiyama and Kazuyoshi Kawazu


Chitosan derivatives classed into three groups according to their molecular size were examined for the relationships between degree of N -acetylation and phytoalexin (+)-pisatin-inducing activity in pea epicotyl assay. Partially N-deacetylated chitin with degree of N-acetylation 56% (DAC 56%) was the most potent inducer of (+)-pisatin among sparingly water-soluble polysaccharides including chitosan (degree of N -acetylation 0%), DAC 32%, and chitin. Among the intermediate-sized molecules obtained from degradation of the chitosan and the DACs by NaNO2, the chitosan fragment was the most powerful elicitor for (+)-pisatin induc­tion. The NaNO2-degraded DAC 56% exhibited a com parable activity to the NaNO2 -degrad-ed chitosan at low concentrations. The NaNO2-degraded fragments as well as the native poly­mer induced a relatively high amount of (+)-pisatin, while the acetone precipitate of chitosan oligomers from the HCl-catalyzed hydrolysate and their partially N-acetylated derivatives ex­hibited a moderate (+)-pisatin-inducing activity. These findings suggest the possibility that partially N-deacetylated chitin fragments could be one of the most promising elicitors in the pea system.

Received: 1994-1-14
Revised: 1994-2-24
Published Online: 2014-6-2
Published in Print: 1994-6-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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