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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Carbohydrates Protect Mitochondrial F0F1-ATPase Complex against Thermal Inactivation

  • Jorge Saad-Nehme , Jerson L. Silva and Jose Roberto Meyer-Fernandes EMAIL logo

Abstract

Organisms and cellular systems are required to adapt to stress conditions like high temperature, often responding by accumulating organic solutes, such as sugars. This accumulation is associated with the effectiveness of these osmolytes in minimizing protein denaturation and membrane damage under stress conditions. In this work, we have studied the effect of sugars on the protection against thermal inactivation of mitochondrial F0F1-ATPase complex, in preparations of submitochondrial particles containing or depleted of inhibitor protein. We observed that after 15 min of pre-incubation at 70 °C of latent MgATP-submitochondrial particles (with inhibitor protein) in the presence of 1.5 м of sucrose or trehalose, or 3.0 ᴍ of glucose or fructose, about 80% of enzyme activity remained active. In the same conditions, but in the absence of sugars, the activity of the particles was completely abolished. Submitochondrial particles depleted of the inhibitor protein (AS-particles) were almost completely inactivated after 3 min of pre-incubation at 70 °C in the absence of sugars and more than 60% of the enzyme activity remained active when these particles were pre-incubated in the presence of sugars. In such condition, the enzyme acquires a more compact and heat-stable conformation. Sugars, as well as the inhibitor protein, inhibit reversibly F0F1-ATPase complex activity and protect this enzyme against inactivation by high temperature. Interestingly, the protection, promoted by sugars, of particles containing inhibitor protein is higher than of particles depleted of inhibitor protein, suggesting a synergism between sugar and inhibitor protein.

Received: 2000-2-10
Revised: 2000-3-31
Published Online: 2014-6-2
Published in Print: 2000-8-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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