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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Effect of CDP-choline on Hippocampal Acetylcholinesterase and Na+,K+-ATPase in Adult and Aged Rats

  • Christos Plataras , Panagoula Angelogianni and Stylianos Tsakiris EMAIL logo

The aim of this study was to investigate the effect of different cytidine-5′-diphosphocholine (CDP-choline) concentrations (0.1-1 mm) on acetylcholinesterase (AChE), (Na+,K+)-ATPase and Mg2+-ATPase activities in homogenates of adult and aged rat hippocampi. Tissues were homogenised, centrifuged at 1000 × g for 10 min and in the supernatant, AChE activity and Na+,K+-ATPase and Mg2+-ATPase activities were determined according to Ellman’s method and Bowler’s and Tirri’s method, respectively. After an 1-3 h preincubation of the homogenised tissue with CDP-choline, a maximal AChE stimulation of about 25% for both adult and aged rats (p < 0.001) and a Na+,K+-ATPase activation of about 50% for adult rats (p < 0.001) and about 60% for aged rats (p < 0.001) were observed, while hippocampal Mg2+- ATPase activity was not influenced in either adult or aged animals. It is suggested that: CDP-choline can restore hippocampal AChE and Na+,K+-ATPase activities in the aged rat and thus it may play a role in improving memory performance which is impaired by aging and some neuronal disturbances.

Received: 2002-10-15
Revised: 2002-11-11
Published Online: 2014-6-2
Published in Print: 2003-4-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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