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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Improvement of Carotenoid-Synthesizing Yeast Rhodotorula rubra by Chemical Mutagenesis

  • Ginka I. Frengova , Emilina D. Simova EMAIL logo and Dora M. Beshkova

A mutant Rhodotorula rubra with enhanced carotenoid-synthesizing activity for synthesizing total carotenoids and β-carotene was obtained by N-methyl-N′-nitro-N-nitrosoguanidine mutagenesis. When co-cultivated with yogurt starter bacteria (Lactobacillus bulgaricus + Streptococcus thermophilus) in whey ultrafiltrate it produced 15.7 mg total carotenoids l-1 culture fluid or 946 μg total carotenoids g-1 dry cells of which 71% was β-carotene. Grown as a monoculture in glucose substrate, the mutant shown 1.4 times lower carotenoid-synthesizing activity, and the relative share of β-carotene in the total carotenoids was lower (63%). The individual pigments torulene and torularhodin were identified, whose mass fractions were (29% and 7%) and (24% and 4%), respectively, for the mutant grown as a monoculture and as a mixed culture with the yogurt bacteria.

Received: 2003-5-23
Revised: 2003-7-3
Published Online: 2014-6-2
Published in Print: 2004-2-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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