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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Cytotoxic Abietane Diterpenes from Hyptis martiusii Benth.

Edigênia Cavalcante da Cruz Araújo, Mary Anne S. Lima, Raquel C. Montenegro, Marcelle Nogueira, Letícia V. Costa-Lotufo, Cláudia Pessoa, Manoel Odorico de Moraes and Edilberto R. Silveira

From roots of Hyptis martiusii Benth. two tanshinone diterpenes were isolated, the new 7β-hydroxy-11,14-dioxoabieta-8,12-diene (1) in addition to the known 7α-acetoxy-12-hydroxy- 11,14-dioxoabieta-8,12-diene (7α-acetoxyroyleanone) (2). Structures of 1 and 2 were established by spectroscopic means. The cytotoxic activity against five cancer cell lines was evaluated. Compounds 1 and 2 displayed considerable cytotoxic activity against several cancer cell lines with IC50 values in the range of 3.1 to 11.5 μg/ml and 0.9 to 7.6 μg/ml, respectively. The cytotoxic activity seemed to be related to inhibition of DNA synthesis, as revealed by the reduction of 5-bromo-2′-deoxyuridine incorporation and induction of apoptosis, as indicated by the acridine orange/ethidium bromide assay and morphological changes after 24 h of incubation in leukemic cells.

Received: 2005-11-10
Published Online: 2014-6-2
Published in Print: 2006-4-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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