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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

The Influence of Auxins on the Biosynthesis of Isoprene Derivatives in Callus Cultures of Vaccinium corymbosum var. bluecrop

  • Piotr Migas EMAIL logo , Maria Łuczkiewicz and Wojciech Cisowski

Callus cultures of Vaccinium corymbosum var. bluecrop were optimized for their isoprene derivatives production by supplementing Schenk-Hildebrandt (SH) medium with constant concentration of kinetin (2.32 μᴍ) and two different amounts of selected auxins. Every auxin, except for IBA, used in 10-time higher concentration (2,4D, NAA, IAA, NOA) stimulated biosynthesis of β-sitosterol and inhibited triterpene synthesis. Quantitative analysis of isoprene derivatives in callus biomass collected on the 25th day of the experiment proved that the analyzed callus of Vaccinium corymbosum var. bluecrop synthesized the highest amount of isoprene derivatives after subculturing on SH medium modified with 22.6 μᴍ of 2,4D and 2.32 μᴍ of kinetin.

Received: 2005-11-15
Revised: 2006-1-23
Published Online: 2014-6-2
Published in Print: 2006-8-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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