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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Influence of Interferons on the Repair of UV-Damaged DNA

  • Vania L. Tsoncheva , Kristina A. Todorova , Ivan G. Ivanov and Vera A. Maximova

The capacity for nucleotide excision repair of a normal (WISH) and three tumour (MCF-7, HeLa, Namalva) cell lines treated with human recombinant interferons (hrIFN-α and hrIFN-γ) was compared by the host cell reactivation assay. The cells were transfected with in vitro UV-damaged plasmid DNA (pEGFP-N1). The repair capacity was determined by measuring the fluorescence intensity of the expressed marker protein in total cell lysates. The correlation between the interferon-induced NO content and the suppressive effect of interferons on DNA repair was shown. The decrease of repair activity and NO induction by hrIFN-α were greatest in WISH, followed by MCF-7, Namalva and HeLa cells, whereas hrIFN-γ was the best NO inducer and inhibitor for the repair of Namalva, followed by WISH, MCF-7 and HeLa cells. Our data clearly show that the two types of interferon have a strong inhibitory effect on the repair of UV-damaged DNA and this effect is cell type-dependent

Received: 2007-6-29
Revised: 2007-10-2
Published Online: 2014-6-2
Published in Print: 2008-4-1

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