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BY-NC-ND 3.0 license Open Access Published by De Gruyter June 2, 2014

Cloning and Characterization of a cDNA Encoding Type 1 Diacylglycerol Acyltransferase from Sunflower (Helianthus annuus L.)

  • Li Sun , Chao Ouyang , Shanglong Kou , Shenghua Wang , Yunyi Yao , Tong Peng , Ying Xu , Lin Tang and Fang Chen EMAIL logo

A full-length cDNA encoding a putative diacylglycerol acyltransferase (DGAT; EC was obtained from sunflower (Helianthus annuus L.) seeds. The 1524-bp open reading frame of this cDNA, designated as HaDGAT1, encodes a protein of 507 amino acids with a molecular mass of 58.5 kDa showing high homology to DGAT1 enzymes of other plants. The protein characters, such as a predicted structure with a long N-terminal hydrophilic domain followed by 9 transmembrane domains, acyl-CoA-binding signature, diacylglycerol (DAG)-binding and putative endoplasmic reticulum retrieval motifs (ER-DIR), also indicated that HaDGAT belongs to the DGAT1 family. HaDGAT1 is expressed in all plant tissues especially in developing seeds. Expression of recombinant HaDGAT1 in yeast showed an 1.76-fold increase of total fatty acids, especially unsaturated fatty acids such as palmitoleic acid (enhanced by 86.6%) and oleic acid (enhanced by 81.6%).

Received: 2010-4-9
Revised: 2010-8-15
Published Online: 2014-6-2
Published in Print: 2011-2-1

© 1946 – 2014: Verlag der Zeitschrift für Naturforschung

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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