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Licensed Unlicensed Requires Authentication Published by De Gruyter March 17, 2016

Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines

Ramazan Erenler, Koksal Pabuccu, Ayse Sahin Yaglioglu, Ibrahim Demirtas and Fatih Gul

Abstract

In this study, the effect of Mougeotia nummuloides and Spirulina major on Vero cells (African green monkey kidney), C6 cells (rat brain tumor cells) and HeLa cells (human uterus carcinoma) was investigated in vitro. The antiproliferative effect of the methanol extract of M. nummuloides and S. major compared with 5-fluorourasil (5-FU) and cisplatin was tested at various concentrations using the BrdU Cell Proliferation ELISA. Both M. nummuloides and S. major extracts significantly inhibited the proliferation of Vero, HeLa and C6 cancer cell lines with IC50 and IC75 values. The M. nummuloides extract exhibited higher activity than 5-FU and cisplatin on Vero and C6 cells at high concentrations. The S. major extract revealed better antifproliferative activity than standards against Vero cells at 500 μg/mL. The compounds of methanol extracts were determined by GC-MS after the silylation process. Trehalose, monostearin and 1-monopalmitin were detected as major products in the M. nummuloides extract where as in the S. major extract; monostearin, 1-monopalmitin and hexyl alcohol were the main constituents.


Corresponding author: Ramazan Erenler, Faculty of Science and Art, Department of Chemistry, Gaziosmanpasa University, 60250 Tokat, Turkey, E-mail:

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Received: 2016-1-13
Revised: 2016-2-13
Accepted: 2016-2-21
Published Online: 2016-3-17
Published in Print: 2016-3-1

©2016 by De Gruyter

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