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Licensed Unlicensed Requires Authentication Published by De Gruyter May 31, 2019

Evaluation of anti-adipogenic active homoisoflavonoids from Portulaca oleracea

Jung Im Lee , Jung Hwan Oh , Chang-Suk Kong ORCID logo and Youngwan Seo EMAIL logo


This study was performed to isolate antiobesity components from the crude extract of Portulaca oleracea. The crude extract was partitioned into n-hexane, 85% aqueous methanol, n-butanol, and water fractions. Their effects on adipogenic differentiation were evaluated in 3T3-L1 cells. Among the solvent fractions from P. olearacea, the 85% aq. MeOH effectively reduced the levels of lipid accumulation. Further purification of 85% aq. MeOH led to the isolation of the known homoisoflavonoids 1–4, as the active substances. The administration of homoisoflavonoids to adipocyte cells decreased the lipid accumulation and glucose consumption and increased the release of glycerol into culture medium. In particular, homoisoflavonoid 3 effectively down-regulated the adipogenic transcription genes such as peroxisome proliferator activated receptor-γ (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPα), and adipogenic target genes such as fatty acid binding protein 4 (FABP4), fatty acid transport protein 1 (FATP1), and acyl-CoA synthase 1 (ACS1).


This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1A2B4009588). Also, this work was supported by the National Research Foundation of Korea (NRF) and the Center for Women In Science, Engineering and Technology (WISET) Grant funded by the Ministry of Science and ICT (MSIT) under the Program for Returners into R&D (WISET-2018-792).


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Received: 2018-07-27
Revised: 2019-01-17
Accepted: 2019-03-16
Published Online: 2019-05-31
Published in Print: 2019-09-25

©2019 Walter de Gruyter GmbH, Berlin/Boston

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