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Licensed Unlicensed Requires Authentication Published by De Gruyter March 12, 2020

Activity-guided isolation of cholinesterase inhibitors quercetin, rutin and kaempferol from Prunus persica fruit

Dominik Szwajgier , Kamila Borowiec ORCID logo EMAIL logo and Josef Zapp

Abstract

Prunus persica fruit is a source of anti-cholinesterase agents and led to an increase of acetylcholine levels in brain tissue as a usefull tool in the Alzheimer’s disease therapy. This study aimed to propose a convenient method for the purification of cholinesterase inhibitors from P. persica water extract. Water extract from the fruit was ultrafiltered (0.2 μm→5 kDa→3 kDa) followed by preparative chromatography (Sephadex LH-20, high pressure C18) and high pressure analytical C18 chromatography. The chemical structures of inhibitors were confirmed using infrared and 1H-NMR spectroscopies. The anti-acetylcholinesterase activity was measured using the colorimetric method in fractions obtained after each stage of purification. Polyphenolic cholinesterase inhibitors identified in peach fruit were kaempferol, quercetin and quercetin-3-O-rhamnoglucoside (rutin). The relatively fast purification procedure elaborated in this work can be adopted for the isolation of phenolic cholinesterase inhibitors from fruit extracts related to P. persica fruit.

  1. Disclosure statement: No potential conflict of interest was reported by the authors.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2019-0079).


Received: 2019-04-09
Revised: 2020-01-28
Accepted: 2020-02-03
Published Online: 2020-03-12
Published in Print: 2020-03-26

©2020 Walter de Gruyter GmbH, Berlin/Boston

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