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Licensed Unlicensed Requires Authentication Published by De Gruyter September 1, 2021

Role of Hypericum perforatum oil and pomegranate seed oil in wound healing: an in vitro study

  • Ebru Uzunhisarcıklı ORCID logo EMAIL logo and Mükerrem Betül Yerer


This study aimed to investigate the wound-healing efficacy of Hypericum perforatum and pomegranate seed extract oil combined with curcumin as an anti inflammatory agent. A series of experiments were carried out to determine effective concentrations for H. perforatum oil, pomegranate seed extract oil, and curcumin. Ibuprofen was used as a positive control. The wound-healing effects of the applied compounds were tested according to the migration experiment model performed in HaCaT cells. A real-time cell analyzer (xCELLigence) was used to determine the cytotoxic/proliferative effects of H. perforatum, pomegranate seed oil, ibuprofen, and curcumin in HaCaT cells alone and their combined use at specified concentrations. After examining the noncytotoxic concentrations of H. perforatum oil, pomegranate seed oil, curcumin, and ibuprofen, migration experiments were performed to examine the wound healing properties. According to the results, the wound-healing efficacy of curcumin and H. perforatum combination was better than ibuprofen combinations. Also, according to the results, the wound-healing efficacy of curcumin and pomegranate seed oil combination was better than ibuprofen combinations. It was concluded that both oils had improved wound-healing properties in combination with curcumin or Ibuprofen.

Corresponding author: Ebru Uzunhisarcıklı, Department of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey, E-mail:


We thank to Atabay Pharmaceuticals and Fine Chemicals Inc. for providing drug substance. Also, we thank to Seda Dökücü and Furkan Kağan for their help on experiments.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Conflict of interest statement: There are no conflicts of interest.


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Supplementary material

The online version of this article offers supplementary material (

Received: 2020-12-18
Accepted: 2021-08-19
Published Online: 2021-09-01
Published in Print: 2022-05-25

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