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Licensed Unlicensed Requires Authentication Published by De Gruyter September 28, 2021

()-Brunneusine, a new phenolic compound with antibacterial properties in aqueous medium from the leaves of Agelanthus brunneus (Engl.) Tiegh (LORANTHACEAE)

  • Moifo Kuete Thomas Wieland , Ambassa Pantaleon , Moungang Luciane Marlyse , Ngameni Bathelemy EMAIL logo , Storr E. Thomas , Ngadjui Tchaleu Bonaventure and Stephenson G. Richard EMAIL logo


Agelanthus brunneus (Loranthaceae) is a hemiparasitic plant growing on Senna siamea (Fabaceae). The chemical investigation of its leaves and flowers led to the isolation of one new phenolic compound namely (−)-brunneusine (1), together with 13 known compounds. The crude leaves and flowers extracts (CLE and CFLE) with their ethyl acetate fractions (EAFL and EAFFL) and some isolated compounds (1–3; 8–9 and 11–14) have been tested on four bacterial species of sanitary importance isolated in an aquatic environment. All the samples except compound 3 showed antibacterial activity with MICs ranging from 0.43 to 8.88.103 µg/mL and MBCs from 0.43 to 3.55.103 µg/mL. Compounds 9 and 14 showed better activity on all bacterial species tested with MICs ranging from 0.43 to 27.77 µg/mL. Only CLE, EAFL and compounds 14, 2, 8 and 9 showed bactericidal effects on all bacterial species tested.

Corresponding authors: Ngameni Bathelemy, Department of Pharmacognosy and Pharmaceutical Chemistry, University of Yaoundé I, Yaoundé, P.O. Box 1364, Cameroon; and School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK, E-mail: ; and Stephenson G. Richard, School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK, E-mail:

Funding source: UEA

Award Identifier / Grant number: CHE 21A3


B. Ngameni, S. E. Thomas and S. G. Richard acknowledge Open Lab Africa through this exchange research program. The authors are also grateful to the School of Chemistry, Faculty of Science, University of East Anglia, UK for research facilities and support with the experimental equipment and Dr. C.J. Macdonald, NMR Facility Manager, School of Chemistry for technical advice and training.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This research was supported by the UEA Vice Chancellor’s Global Challenges Research Fellowships Scheme; Code: CHE 21A3.

  3. Conflict of interest statement: The authors declare no conflict of interest.


HR-ESI-MS, IR as well as 1D and 2D NMR spectra of compounds 1–3; 6–14 are available as supplementary material.


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Supplementary Material

The online version of this article offers supplementary material (

Received: 2021-05-18
Accepted: 2021-09-13
Published Online: 2021-09-28
Published in Print: 2022-03-28

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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