Abstract
Spin-labeling active compounds is a convenient way to prepare them for EPR spectroscopy with minimal alteration of the target molecule. In this study we present the labeling reaction of dexamethasone (Dx) with either TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy) or PCA (3-(carboxy)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy) with high yields. According to NMR data, both labels are attached at the primary hydroxy group of the steroid. In subsequent spin-stability measurements both compounds were applied onto HaCaT cells. When the signal of Dx-TEMPO decreased below the detection limit within 3 h, the signal of Dx-PCA remained stable for the same period of time.
Dedicated to: Eckart Rühl on the occasion of his 60th birthday.
Acknowledgements
The authors acknowledge support from Deutsche Forschungsgemeinschaft (DFG)/German Research Foundation via SFB 1112, Projects A02 and B01.
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The online version of this article offers supplementary material (https://doi.org/10.1515/zpch-2017-1076).
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