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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access September 1, 2004

Determination of aristolochic acid by capillary zone electrophoresis

František Kvasnička, Rudolf Ševčík, Michal Voldřich and Jana Krátká
From the journal Open Chemistry


A simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5 minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC.

[1] G.M. Lord, T. Cook, V.M. Arlt, H.H. Schmeiser, G. Williams and C.D. Pusey: “Urothelial malignant disease and Chinese herbal nephropathy”, Lancet, Vol. 358, (2001), pp. 1515–1516. in Google Scholar

[2] J.P. Cosyns: “Aristolochic acid and ‘Chinese herbs nephropathy’—A review of the evidence to date”, Drug Safety, Vol. 26, (2003) pp. 33–48. in Google Scholar

[3] J.R. Ioset, G.E. Raoelison and K. Hostettmann: “Detection of aristolochic acid in Chinese phytomedicines and dietary supplements used as slimming regimens”, Food Chem. Toxicol., Vol. 41, (2003) pp. 29–36. in Google Scholar

[4] S. Zhou, H. Koh, Y. Gao, Z. Gong and E.J.D Lee: “Herbal bioactivation: the good, the bad and the ugly”, Life Sci., Vol. 74, (2004), pp. 935–968. in Google Scholar

[5] J.H. Jon, S.S. Chen and T.S. Wu: “Facile reversed-phase HPLC resolution and quantitative determination of aristolochic acid and aristolactam analogues in traditional Chinese medicine”, J. Liq. Chromatogr. R T., Vol. 26, (2003) pp. 3067–3078. Search in Google Scholar

[6] J.R. Ioset, G.E. Raoelison and K. Hostettmann: “An LC/DAD-UV/MS method for the rapid detection of aristolochic acid in plant preparations”, Planta Med., Vol. 68, (2002), pp. 856–858. in Google Scholar

[7] T.Y. Lee, M.L. Wu, J.F. Deng and D.F. Hwang: “High-performance liquid chromatographic determination for aristolochic acid in medicinal plants and slimming products”, J. Chromatogr., Vol. 766, (2001), pp. 169–174. Search in Google Scholar

[8] E.S. Ong and S.O. Woo: “Determination of aristolochic acids in medicinal plants (Chinese) prepared medicine using capillary zone electrophoresis”, Electrophoresis, Vol. 22, (2001) pp. 2236–2241.<2236::AID-ELPS2236>3.0.CO;2-T10.1002/1522-2683(20017)22:11<2236::AID-ELPS2236>3.0.CO;2-TSearch in Google Scholar

[9] H.A. Priestap, S.L. Iglesias, M.F. Desimone and L.E. Diaz: “Determination of aristolochic acids by capillary electrophoresis”, J. Capillary Electrop., Vol. 8, (2003), pp. 39–43. Search in Google Scholar

[10] Z. Sun, L. Liu, X. Zheng, Ch. Fan, Q. Wang and G. Li: “An easy and rapid method to determine aristolochic acids I and II with high sensitivity”, Anal. Bioanal. Chem., Vol. 378, (2004) pp. 388–390. in Google Scholar

Published Online: 2004-9-1
Published in Print: 2004-9-1

© 2004 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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