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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access September 1, 2004

Determination of aristolochic acid by capillary zone electrophoresis

František Kvasnička, Rudolf Ševčík, Michal Voldřich and Jana Krátká
From the journal Open Chemistry

Abstract

A simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5 minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC.

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Published Online: 2004-9-1
Published in Print: 2004-9-1

© 2004 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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